简介：AbstractObjective:Pseudomonas aeruginosa (P. aeruginosa) contains a hierarchy of quorum sensing (QS) network, consisting of the las, rhl and pqs systems, which play a key role in coordinating the expression of virulence factors. PA2146 was found to be associated with P. aeruginosa pathogenicity in macrophage and host Immune response. The aim of this study was to investigate the effects of PA2146 on the virulence of P. aeruginosa and explore its mechanism.Methods:PA2146 gene knockout strain and complement strain of P. aeruginosa PAO1 were constructed. The biomass of biofilm was detected by crystal violet staining; the virulence factors were measured, including pyocyanin, rhamnolipid, LasA elastase, LasB elastase and hemolytic activity; RNA-seq and label-free relative quantitative proteomics analyses were carried out to test the influence of PA2146 on transcriptomics and proteomics. This study was approved by the Institutional Review Board of the Third Xiangya Hospital, Central South University, China (approval No. 2019-S021).Results:PA2146-deficient strains showed reduced biofilm formation and increased pyocyanin, rhamnolipid, LasA elastase, LasB elastase, and hemolytic activity, as well as increased motility, compared with the wild-type strain. RNA-seq and label-free relative quantitative proteomics analyses revealed that PA2146 repressed the transcription of several genes that are integral to the pqs system and to pyocyanin biosynthesis, and increased the expression of MexEF-OprN efflux pump components at the gene and protein level.Conclusion:PA2146 gene was found to inhibit the pqs system. PA2146 may affect quorum sensing by directly inhibiting the pqs system or by enhancing the expression of MexEF-OprN efflux pump components, thereby promoting efflux of 2-heptyl-4(1H)-quinolone, a Pseudomonas quinolone signal precursor, and thus affecting P. aeruginosa virulence.

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简介：(QS)治安法官察觉到是细菌用称为autoinducers的细胞外的信号由交流的细菌的房间房间通讯进程。二个QS系统在EscherichiacoliK-12被识别了，包括一个未经触动的QS系统2那被周期的安培(营地)营地受体蛋白质(CRP)刺激建筑群和一个部分QS系统1那由对另外的微生物引起的种类产生的信号作出回应的SdiA(房间部门禁止者的suppressor)组成。在QS之间的关系在E的系统1和系统2。然而，coli仍然保持阴暗。这里，我们显示出那EAL域蛋白质，由ydiV，和营地编码了在E涉及在二个QS系统之间的相互作用。coli。sdiA和ydiV的表示被葡萄糖禁止。SdiA在一个剂量依赖者，而是nonspecific绑在ydiV倡导者区域，举止；细胞外的autoinducer1以一种sdiA依赖的方式从另外的种类刺激ydiV表示。而且，我们发现双sdiA-ydiV变化，然而并非单个变化，在导致QS系统2的抑制的细胞内部的营地集中引起2褶层减少。这些结果显示对重要环境暗示作出回应的发信号的小径例如autoinducers和葡萄糖，在E为他们的控制一起被连接。coli。

简介：(QS)在治安法官察觉到处理，细菌由利用响应许多环境暗示称为autoinducers的小发信号的分子调整基因表示。Autoinducer2(AI-2)，表明分子的QS建议涉及interspecies通讯，被克否定、克积极的细菌的许多种类生产。在Escherichiacoli和沙门氏菌typhimurium，细胞外的AI-2被lsr操纵子编码的transporter进口进房间。lsr操纵子在上游，有编码LsrR的分叉地抄录的基因，它以前被报导镇压lsr操纵子和自己的抄写。这里，我们第一次证明了LsrR镇压lsr操纵子和自己由的抄写对他们用胶化移动和DNase的倡导者直接有约束力我footprinting试金。牛乳糖记者试金进一步建议在lsrR和lsrA倡导者区域的二个主题为LsrR绑定是关键的。而且，与结论一致，那phosphorylatedAI-2能在以前的研究减轻LsrR的压抑，我们phospho-AI-2显示的数据表演不能在vitro绑在它的自己的倡导者的LsrR。

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简介：Asuitablefiberopticsensingarrayembeddedinthesmartstructuretomonitorquasi-distrbutedfrocesonthestructrueinpresentedinthispaper.Artificialneuralnetworksareusedinprocessingoffiberopticsensingarraysignals.Fundamentalexperimentshavebeendoneandtheresultsarealsogiven.

简介：Variousplatforms,suchassatellite,aircraft,ground-based,someemergingaspects(e.g.internet)haveresultedinadramaticimprovementinthecapabilitiesofearthobservations(EO).Thenumerousremotesensingdatapromoteanenhancedpossibilitytoassess,monitor,andpredictthedynamicsofland-covers,anthropologicprocesses,andinfluencetotheenvironments.Nonetheless,thepropertiesofthedataacquiredbysuchdiversesourcesposechallengestotheprocessingmethodologies,andhence,developmentofaseriesofnewmethodsfortheanalysisofremotesensingimagesisrequired,TheaimofthisspecialissueofGeospatialInformationScienceistodevelopnewideasandtechnologiestofacilitatetheutilityofremotesensingdataandtofurtherexploreitspotentialinvariousapplications.

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简介：Theadditionofenvironmentalsensing,intheformofdepthsensing,tothecontrolofpowered,lower-limbprostheseswasproposed.Currently,thepredictionofwhichlocomotionmode-basedcontrollertousehasbeenperformedusingEMGfromtheresiduallimbandkineticsandkinematicsfromtheprosthesis.However,duetothevariabilityofEMG,kinetics,andkinematicsacrosstrials,days,andsubjects,itisdifficulttoproducerobustpredictionswithlowerrorrates.Thisworkfocusedonthede-

简介：Theforcesensingresistor(FSR)anditsconstructionandcharacteristicaredescribed.Byusingtheoptimalelectronicinterface,theendresultwhichisadirectproportionalitybetweenforceandvoltageisobtained.Thecircuitsofapplicationforforceandpositionmeasurementsintheroboticcontrolaregiven.TheexperimentthatFSRsareplacedonthefingersofBH-1dexteroushandastactilesensorstomeasurethecontactingforcesshowsFSR'sforcesensitivityisoptimizedforuseinthecontrolofrobotcontactingwithenvironment.

简介：ThepurposeofthispaperistostudytheRSdatawebservicesandrelatedsubjectsofdatastorageanddatamanagement.Basedonananalysisofthepresentsituationanddevelopmenttrendofstorageandmanagementofrasterdataandwebservicetechnology,amanagementandservicesystemarchitectureforRemoteSensingrasterdatabasedonwebservicetechnologieswasdeveloped,theimplementationmethodologiesofthekeytechnologyofthesystemwereexploredandaprototypeofthesystemwasillustrated.

简介：Ｔｈｅａｐｐｌｉｃａｔｉｏｎｏｆｍｉｃｒｏｗａｖｅｒｅｍｏｔｅｓｅｎｓｉｎｇｔｅｃｈｎｏｌｏｇｙｉｎｒｏｃｋ　ｍｅｃｈａｎｉｃｓＮａｉ－ＧｕａｎｇＧＥＮＧ（耿乃光）；Ｚｈｅｎｇ－ＦａｎｇＦＡＮ（樊正芳）；Ｑｕａｎ－ＱｕａｎＪＩ（籍全权）；Ｃｈｅｎｇ－...

简介：推迟的沉积的遥感上的量的申请是遥感学习的设计申请的一个重要方面。在这份报纸，厦门海湾被选择为学习区域。遥感数据的十一个不同阶段被选择建立一个量的遥感模型由使用遥感图象和伪同步的测量沉积数据印射推迟的沉积。基于开发的实验统计是在即时推迟的沉积集中和集中以及变换在表面层之间建模的平均tidally的推迟的沉积之间的变换模型推迟的沉积集中和平均深度的推迟的沉积集中。在这个基础上，量的应用程序推迟的沉积的遥感上的综合模型被开发。由象多时间的遥感图象一样使用这个模型，平均的多年推迟了厦门海湾的沉积集中被预言。在模型预言和多年平均的观察数据表演之间的比较象附近的地点的集中差别一样推迟了学习地点的沉积集中能被遥感模型很好与21.61%的错误率或更少预言，它能满足隧道免职计算的设计要求。

简介：-ThedataoflandsatTMofmulti-temporalforLingdingyangEstuary,PearlRiverinChinaisfirstlyusedwithsuspendedsedimentconcentrationoffieldmeasurementtoestablishacorrelativemodelequation.AftertheratioprocessingofTMdataandatmosphericcorrection,theimagesofsuspendedsedimentconcentrationofdifferenttemporalsareexportedfromtheimageprocessingsystemsAREISIIandIII.Theseimagesexpressthecharacteristicsofsuspendedsedimentdistribution,themodeofsedimenttransportandtheextentofdispersionundertheactionsoftidalcurrentandwindconditionofdifferentseasons.

简介：Inordertoimprovetheinterpretationoftheearthsystemmicrowaveremotesensing,theresearchofmicrowavespectrumcharacteristicsofthegroundtruth(earthobjects)wascarriedoutinlaboratory.Alaboratoryformicrowaveremotesensingoftheearthobjectshasbeenconstructedtoimprovetheremotesensinglevel,thelaboratoryconsistsoffourparts:themeasuringsystemofdielectricconstants,themicrowaveemissivitymeter,themicrowavereflectometerandthemicrowaveremotesensingsimulationexperimentinfield.Inthispaper,theprincipleofmeasurement,thecorrectionofnearfieldprocess,thestructureofinstrument,thecalibrationmethodandthemeasurementoftheearthsubstances,includingsoil,waterandoil,arediscussed.Thelabora-torymaysupplytheconditionformeasuringtheparametersofthcearthsubstanceremotesensingandhelptointerprettheremotesensingdata.

简介：Compressivesensing(CS)hasemergedasanovelsamplingframeworkwhichenablessparsesignalacquisitionandreconstructionwithfewermeasurementsbelowtheNyquistrate.AnimportantissueforCSistheconstructionofmeasurementmatrixorsensingmatrix.Anewdeterministicsensingmatrix,namedasOOC-B,isproposedbyexploitingopticalorthogonalcodes(OOCs),BernoullimatrixandSingerstructure,whichhastheentriesof0,+1and-1beforenormalization.Wehaveproventhatthedesigneddeterministicmatrixisasymptoticallyoptimal.Inaddition,theproposeddeterministicsensingmatrixisappliedtodirectionofarrival(DOA)estimationofnarrowbandsignalsbyCSarrays(CSA)processingandCSrecovery.TheoreticalanalysisandsimulationresultsshowthattheproposedsensingmatrixhasgoodperformanceforDOAestimation.ItisveryeffectiveforsimplifyinghardwarestructureanddecreasingcomputationalcomplexityinDOAestimationbyCSAprocessing.Besides,lowerrootmeansquareerror(RMSE)andbiasareobtainedinDOAestimationbyCSrecovery.