简介:2CasesofMediterraneanspottedfeverinFrancewerereportedinthispaper.Bothhadtraveledintheendemicareasbeforeonset.Theirclinicalmanifestationsincludedfever,headache,myalgia,eruptionandblackeschar.SerologictestssuchasWeil-Felixreaction,Indirectimmunoflurescenceandcomplementfixationconfirmedthediagnosis.Thepatientsrecoveredwithoutcomplicationsandweredischargedfromhospitalaftereffectivetreatment.Simultaneously,theepidemiology,clinicaldiagnosis,treatmentandpreventionofthediseasewerereviewedandthepossibilityofthediseasebeingintroducedintothisterritorywasdiscussed.Theauthorheldthatattentionshouldbepaidtotheprophylaxisandtreatmentofthediseaseintheinternationaltravelhealthcare.
简介:Alightbrownspotted-leafmutantofricewasisolatedfromanethanemethylsulfonate(EMS)-inducedIR64mutantbank.Themutant,designatedaslbsl1(lightbrownspotted-leaf1),displayedlightbrownspotinthewholegrowthperiodfromthefirstleaftotheflagleafundernaturalsummerfieldconditions.Agronomictraitsincludingplantheight,growthduration,numberoffilledgrainsperpanicle,seed-settingrateand1000-grainweightofthemutantweresignificantlyaffected.Geneticanalysisshowedthatthemutationwascontrolledbyasinglerecessivegene,tentativelynamedlbsl1(t),whichwasmappedtotheshortarmofchromosome6.Bydevelopingsimplesequencerepeat(SSR)markers,thegenewasfinallydelimitedtoanintervalof130kbbetweenmarkersRM586andRM588.Thelbsl1(t)geneislikelyanovelricespotted-leafgenesincenoothersimilargeneshavebeenidentifiednearthechromosomalregion.Thegeneticdataandrecombinationpopulationsprovidedwillfacilitatefurtherfine-mappingandcloningofthegene.
简介:病毒的紧张的坏死(VNN)在海洋的鱼引起高死亡,特别在grouper,全球并且在中国。因为没有有效疫苗或药处理VNN,早察觉和预防是重要的堵住它的爆发。在这研究,反向的抄写聚合酶链反应(RT-PCR)为VNN病原体的快速、方便、敏感的察觉被开发,紧张的坏死病毒(NNV),在grouper。整个过程从RNA抽取在3.5h以内被完成到PCR产品可视化。这个方法的察觉限制是NNVRNA标准的200个拷贝,它对应于病毒粒子的200个拷贝。这个RT-PCR方法对NNV察觉没有特定对另外的鱼跨反应病毒的疾病病原体例如传染胰腺的坏死病毒(IPNV),传染造血的坏死病毒(IHNV),鲤鱼病毒(SVCV)的春天viraemia,流行于家畜的造血的坏死病毒(EHNV),并且大黄croakeriridovirus(LYCIV)。与这个方法,看到橘子的grouper(Epinepheluscoioides)从育种站与或没有在福建省的VNN流行病的发生的油炸食品被检测。结果都显示出那或当从没有VNN的二个育种站的40%油炸食品或25%流行病也作为NNV被检测时,从有流行病的发生的二个育种站的93%油炸食品作为积极被诊断积极,显示这个RT-PCR方法能被用于快速,NNV感染的敏感察觉并且在VNN流行警戒适用。
简介:真菌Metarhiziumanisopliae的水、可使乳化的明确的表达的功效孤立ICIPE78在在screenhouse和地条件下面大批出没于普通豆子植物的Tetranychusurticae的人口密度上被评估。合成杀螨剂abamectin作为检查被包括。豆子植物人工地与T被大批出没于。urticae并且允许乘。三个处理在领域试用在screenhouse和1个处理被使用。螨密度被记录在喷洒和每周的postspraying前的2d。豆荚的数字每植物,种子的数字仅仅在screenhouse试用每植物每豆荚,和种子的干燥重量被记录。在screenhouse和地试用,真菌的明确的表达在108conidia/mL的集中适用,杀螨剂作为与控制相比减少了螨的人口密度。在T有重要差别。在在各种各样的spraying以后采样日期的处理之间的urticae人口密度。在screenhouse,螨密度从在对待的叶子的3星期的postspraying在零附近。在4星期的postspraying,在未经治疗的控制(T1)并且在控制水+Silwet-L77(T2)有没有更多的叶子。真菌的明确的表达象在在screenhouse和地实验减少螨密度的abamectin一样有效。在2screenhouse试用期间在生产参数有重要差别,与真菌并且通常有最高的收益的abamectin处理。这研究的结果加重M的潜力。anisopliae为T作为杀螨剂的一种选择孤立ICIPE78。urticae管理。
简介:在紧张的热环境下面,昆虫调整他们的行为和生理学维持关键lifehistory活动并且改进幸存。为交往的种类,相互或对抗,热应力可以以不同方法影响参加者,它然后可以影响生态的关系的结果。在agroecosystems,这可以在尖锐、长期的热可变性下面是在昆虫害虫和他们的对抗parasitoids之间的关系的命运。对这个背景,我们调查了ChilopartellusSwinhoe的不同发展阶段的热忍耐(鳞翅目:Crambidae)并且它的幼虫的parasitoid,Cotesiasesamiae卡梅伦(Hymenoptera:Braconidae)用动态、静态的协议。当为2h暴露了到静态的温度时,更低的致命的温度从9~6吗?
简介:Inthisstudy,threecDNAsequencescorrespondingtocytochromeP450C17(CYP17I),3β-hydroxysteroiddehydrogenase(3β-HSD)andandrogenreceptor(AR)wereisolatedfromspottedseabass(Lateolabraxmaculatus).ThemRNAabundancesofCYP17Iand3β-HSDincreasedfromstageⅡtostageⅤwithasignificantincreaseatstageⅤ,andthehighestabundanceofARmRNAwasdetectedatstageⅢintesticulardevelopmentcycle.CYP17I,3β-HSDandARtranscriptswereobviouslyabundantinsteroidogenesistissuessuchastestis,brain,headkidneyamongothers.StrongandpositivesignalswereobservedmainlyininterstitialcellregionsofL.maculatustestisasweremeasuredwithinsituhybridizationmethod.SignificantincreasesofCYP17Iand3β-HSDtranscriptsweredetectedafter12–48hhCG(humanchorionicgonadotropin)andGnRHa(gonadotropin-releasinghormoneanalogue)treatments.However,anoppositerelationshipwasfoundforARintestisatthesametime.Inaddition,decreasingtrendsofCYP17Iand3β-HSDmRNAwereobservedintestisofL.maculatusinfreshwatergroup(FW)fromday2today6,andmRNAabundanceofARincreasedinbrackishwater(BW)groupfromday4today8.Thesefindingsrevealedthatthesethreesteroidsynthesisgenesareimportfortesticulardevelopment,hormoneandsalinitytreatment,andprovidedalsoaninsightintothemechanismofreproductiveendocrineofL.maculatus.