简介：ThepresentstudywasdesignedtoinvestigatethechemicalconstituentsoftherootsofCudraniafruticosaWight.Compoundswereisolatedbyvariouscolumnchromatographicmethodsincludingsilicagel,polyamide,sephadexLH-20,andsemi-preparativeHPLC.Theirstructureswereelucidatedbyacombinationof1Dand2DNMRtechniques,massspectrometry,andchemicalmethods.Twonewxanthones,CudraxanthoneTandU(1-2),alongwithfourknowncompounds(3-6)wereisolatedfromtherootsofCudraniafruticosaWight.

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简介：试图在安息香perkinsiae的bark学习化学成分。混合物与色析法的方法被孤立的方法，和他们的化学结构被阐明根据光谱数据。结果十混合物作为5-(2-propen-1-one)被孤立并且鉴别-7-methoxy-2-(3,4-methylenedioxyphenyl)benzofuran(1)，1-hydryoxyegonolgentiobioside(2)，obassiosideB(3)，egonol(4)，egonol配糖物(5)，egonolgentiobioside(6)，egonolgentiotrioside(7)，styraxlignolideB(8)，styraxjaponosideC(9)，和masutakeside我(10)。结论混合物1和2是新2-phenylbenzofurans。

简介：Twonewfuranfragmentisomerizedlimonoids,meliazedalidesAandB(compounds1and2),wereisolatedfromthefruitsofMeliaazedarachLinn..TheirchemicalstructureswereelucidatedonthebasisofHR-ESI-MSand1Dand2DNMRdata,whichbelongedtonimbolinin-andtrichilin-class,respectively.Compound2exhibitedweakinhibitoryeffectonNOproductioninlipopolysaccharide(LPS)-activatedRAW264.7macrophageswithIC50being37.41μmol·L^-1.

简介：ThepresentstudywasdesignedtoisolateandcharacterizetheanalgesiccompoundsofArtemisasacrorumLedeb.TheEtOAccrudeextractsfromtheaerialpartsofArtemisasacrorumLedebwereseparatedbychromatographyandthestructuresofnewcompoundswereelucidatedbasedonspectralanalyses.Analgesicactivitiesoftheisolatedcompoundswereassessedinratswithhotplatetestandpawpressureassay.TwonewflavoneC-glycosides,namedasSacrorosideAandB(Compounds1and2)wereisolatedfromtheEtOAccrudeextractoftheaerialpartsofArtemisasacrorumLedeb.Theyshowedsignificantanalgesiceffects.Inconclusion,Compounds1and2arenewnaturalproducts,whichshowsignificantanalgesiceffectsinadose-dependentmanner.

简介：Antifungaldrugresistanceisasignificantclinicalproblem,andantifungalagentsthatcanevaderesistanceareurgentlyneeded.Ininfectiveniches,resistantorganismsoftenco-existedwithsensitiveones,orasubpopulationofantibiotic-susceptibleorganismsmayevolveintoresistantonesduringantibiotictreatmentandeventuallydominatethewholepopulation.Inthisstudy,weestablishedaco-cultureassayinwhichanazole-resistantCandidaalbicansstrainwasmixedwithasusceptiblestrainlabeledwithgreenfluorescentproteintomimicinvivoconditionsandscreenforantifungaldrugs.Fluconazolewasusedasapositivecontroltoverifythevalidityofthisco-cultureassay.FivenaturalmoleculesexhibitedantifungalactivityagainstbothsusceptibleandresistantC.albicans.Twoofthesecompounds,retigericacidB(RAB)andriccardinD(RD),preferentiallyinhibitedC.albicansstrainsinwhichtheeffluxpumpMDR1wasactivated.Thisselectivitywasattributedtogreaterintracellularaccumulationofthedrugsintheresistantstrains.Changesinsterolandlipidcompositionswereobservedintheresistantstrainscomparedtothesusceptiblestrain,andmightincreasecellpermeabilitytoRABandRD.Inaddition,RABandRDinterferedwiththesterolpathway,furtheraggregatingthedecreaseinergosterolinthesterolsynthesispathwayintheMDR1-activatedstrains.Ourfindingshereprovideanalternativeforcombatingresistantpathogenicfungi.

简介：Poriacocos(Schw.)Wolf,animportantmedicinalandfoodfungus,iswellknowninEastAsia.Duetogrowingmarketdemand,longcultivationperiod,andconsumptionofpinetrunkduringcultivation,developingalternativemethodsforproducingP.cocosand/oritsactivecomponentsisofinterest.Inthepresentstudy,theeffectsofdifferentculturemethodsonbiomassandaccumulationoffourtriterpenoidswereinvestigated.Theethanolextractoffermentedmycelium(EFM)wasorallyadministeredtorats.Urineoutputandconcentrationsofelectrolytes(Na~+,K~+,andCl~-)weremeasured.Ourresultsshowedthatmyceliagrewbetterundercontinuousshakingculturecondition(7.5gDW·L~(-1)),andhighertriterpenoidlevelswereaccumulatedintwo-stageculture(112mg·L-1,2.03%).Theoptimalstartingtimeofstaticculturefortriterpenoidyieldwas4~(th)daftershakingculture.SingleadministrationofmiddleandhighdoseofEFMsignificantlyincreasedurineoutput,Na~+andCl~-excretion,andNa~+/K~+ratio.Theseresultssuggestedthatethanolextractofculturedmyceliashowedsignificantdiureticactivityinratsandtwo-stagecultureofP.cocoscouldbeanalternativewaytoproducemyceliaandtriterpenoids.

简介：ScutellariaeRadix(SR),therootofScutellariabaicalensisGeorgi,isusedasanantipyreticdrugandhasbeendemonstratedtohaveanti-inflammatoryactivity.SRisdividedintotwospecifications,'KuQin'(KQ)and'ZiQin'(ZQ),foruseagainstdifferentsymptoms(upperenergizerheatorlowerportionofthetripleenergizer),accordingtothetheoryoftraditionalChinesemedicine(TCM).However,differencesintheefficaciesofthesetwospecificationshavenotbeendetermined.Inthepresentstudy,weaimedtocharacterizethedifferencesintheanti-inflammatoryactivitiesbetweenKQandZQandtoexplorehowtheirdifferencesaremanifestedinlipopolysaccharide(LPS)-inducedmacrophages.Ourresultsshowedthat,inRAW264.7cells(amousemacrophagecelllinederivedfromascites),KQandZQdisplayedanti-inflammatoryeffectsbyinhibitingthereleaseofnitricoxide(NO),inducibleNOS(iNOS),andnuclearfactor-κB(NF-κB)inadose-dependentmannerwithoutdistinction.InNR8383cells(aratalveolarmacrophagecellline),KQandZQdisplayedsimilareffectsonNO,iNOS,andNF-κBasseeninRAW264.7cells,butKQshowedahigherinhibitionrateforNOandiNOSthanthatshownbyZQatthesameconcentration.TheseresultsindicatedthatthereweredifferencesinefficacybetweenKQandZQintreatinglunginflammation.OurfindingsprovidedanexperimentalevidencesupportingthedifferentusesofKQandZQinclinic,asnotedinancientherbalrecords.

简介：Twonewcompounds,(22E)-25-carboxy-8β,14β-epoxy-4α,5α-dihydroxyergosta-2,22-dien-7-one(1)andfusidione(3),alongwithtwoknowncompounds,5α,8α-epidioxyergosta-6,22-diene-3β-ol(2)andmicroperfuranone(4),wereisolatedfromthefermentationproductsofthemarine-sourcedfungusAcremoniumfusidioidesRZ01.Thestructuresofcompounds1and3wereelucidatedbyextensivespectroscopicmethods,especially2DNMR,andtheirabsoluteconfigurationsweresuggestedonthebasisofthecirculardichroismspectralanalysisandtheNOESYdata.BothnewcompoundsshowedinhibitoryactivityagainstHL-60cellswithIC50valuesbeing16.6and44.9μmol·L-1,respectively.