简介：PHProteomicDB是一个写PHP的模块在proteomics帮助研究人员用个人网络站点分享二维的电气泳动胶化数据。不技术或除了一些基础，PHP知识是必要的。PHProteomicDB让一个用户友好的政府连接进入并且更新数据。即时地显示胶化特征，胶化图画，和数的胶化点，他们的相关鉴定在蛋白质数据库指向他们的参考页创造网页。模块在http://www.huvec.com/index.php3是自由地可得到的?rub=Download。

简介：Neisseriameningitidis是侵略meningococcal疾病的代理人，包括服的脑膜炎和败血病。因为不同的同种细胞的组(顺序类型)引起的疾病有他们的自己的流行病学的特征，在N的染色体之中理解差别是重要的。meningitidis同种细胞的组。到这个目的，染色体的一个结构的点阴谋的新奇解释被设计并且适用；在N的染色体之间的准确核苷酸火柴。meningitidisserogroupA紧张Z2491和serogroupB紧张MC58被识别，导致各种各样的结构的区域的说明。知道并且为每N的通常认为的毒力基因。meningitidis紧张然后被分类进这些区域。我们比Z2491做那些发现MC58的毒力基因更趋于到translocated区域(在新顺序上下文的chromosomal片断)，显著地向在translocated区域和在同一直线上的区域之一之间的接口趋于。在在同一直线上的区域以内，毒力基因趋于在准确火柴发生在差距的16kb以内。用在每秒字符数地点聚类的基因的这些趋势的确认是足够地支持的建议这些趋势能被用来集中搜索为并且在这二个有机体毒力基因和致病力的机制理解。

简介：Differentialproteomeprofilesofhumanlungsquamouscarcinomatissuecomparedtopairedtumor-adjacentnormalbronchialepithelialtissuewereestablishedandanalyzedbymeansofimmobilizedpHgradient-basedtwo-dimensionalpolyacrylamidegelelectrophoresis(2-DPAGE)andmatrix-assistedlaserdesorption/ionizationtimeofflightmassspectrometry(MALDI-TOF-MS).Theresultsshowedthatwell-resolved,reproducible2-DEpatternsofhumanlungsquamouscarcinomaandadjacentnormalbronchialepithelialtissueswereobtainedundertheconditionof0.75-ugprotein-load.Theaveragedeviationofspotpositionwas0.733+0.101mminIEFdirection,and0.925+0.207mminSDS-PAGEdirection.Fortumortissue,atotalof1241±88spotsweredetected,987±65spotswerematchedwithanaveragematchingrateof79.5%.Forcontrol,atotalof1190+72spotsweredetected,and875±48spotswerematchedwithanaveragematchingrateof73.5%.Atotalof864±34spotswerematchedbetweentumorsandcontrols.Forty-threedifferentialproteinswerecharacterized:someproteinswererelatedtooncogenes,andothersinvolvedintheregulationofcellcycleandsignaltransduction.Itissuggestedthatthedifferentialproteomicapproachisvaluableformassidentificationofdifferentiallyexpressedproteinsinvolvedinlungcarcinogenesis.Thesedatawillbeusedtoestablishhumanlungcancerproteomedatabasetofurtherstudyhumanlungsquamouscarcinoma.

简介：InFebruary2006,twooutbreaksofhighlypathogenicavianinfluenzaAvirussubtypeH5N1occurredinchickensintwoneighboringdistricts(firstinNandurbarandsecondinJalgaon)ofMaharashtra,India,inaspanof12days.Inthepresentstudy,theneuraminidase(NA)geneofthetwoIndianH5N1isolateswastakenintoconsiderationtofindifthetwostrainsaregeneticallysimilar.PhylogeneticanalysisoftheNAgeneshowedthattheH5N1strainsisolatedfromthetwooutbreakswerenotoriginatedfromthesamesource.ThefirstIndianisolate(Nandubar/7972/06)wasclusteredclosesttoanisolatefromchickeninVietnamin2004,whereasthesecondIndianisolate(Jalgaon/8824/06)showedresemblancetostrainsisolatedfromswaninItalyandIranin2006.Moreover,aminoacidsequenceanalysisshowedvaryinghotspotsforsubstitutionsbetweenthesetwoIndianisolates,andthreesubstitutionswerefoundatfunctionaldomainsites.Secondarystructurechangesduetothesesubstitutionswerealsoreported.ThisstudyrevealsthattheH5N1strainsisolatedfromchickensduring2006birdfluoutbreaksintwoneighboringdistrictsofMaharashtra,Indiaaregeneticallydifferent.