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简介：Xingxiong注射(XXI)是叶片白果树摘录和ligustrazine为心血管、脑血管的疾病的治疗作好准备的一个广泛地使用的中国草药的公式。与药理学研究相比，这个公式上的化学分析和质量控制研究是相对有限的。在现在的学习，高效液体层析结合了四极time-of-flight团spectrometry(HPLC-QTOFMS)方法在XXI被用于成分的全面分析。根据破碎规则和以前的报告，三十白果树flavonoids，四白果树萜烯内酯，和一碱被识别。高效液体层析结合了三倍的四极团spectrometry(HPLC-QQQMS)方法然后被使用确定在XXI的十主要成分。方法确认结果显示发达方法有理想的特性，线性，精确和精确性。白果树flavonoids的全部的内容关于22.05-25.51g·mL−1，白果树萜烯内酯数量分别地关于在XXI样品的六批的4.41-8.70g·mL−1。而且，余弦比率算法和距离大小被采用评估XXI样品的类似，并且结果表明了高质量的一致性。这个工作能在Xingxiong注射的质量控制上提供全面信息，它在一个合理质量控制标准的建立是有用的。

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简介：HyoscyamiSemen,thematuredriedseedofHyoscyamusnigerL.,haslongbeenusedasatraditionalChinesemedicinetotreathumandiseases.HyoscyamiSemenisfoundinlocalmarketsinChina.Inmarkets,sellersandbuyerscommonlyinadvertentlymixtheseedsofH.nigerwiththeseedsofrelatedspeciessuchasHygrophilasalicifolia(Vahl)Nees,AstragaluscomplanatusR.Br.,CuscutaaustralisR.Br.,CuscutachinensisLam.,andImpatiensbalsaminaL.becauseoftheirsimilarmorphologiesorsimilarnames.Thus,developingareliablemethodfordiscriminatingH.nigerseedsfromitsadulterantsisnecessarytoreduceconfusionandensurethesafeuseofHyoscyamiSemen.Thepresentstudywasdesignedtoevaluatetheefficiencyofhigh-resolutionmeltinganalysiscombinedwithDNAbarcoding(Bar-HRM)withinternaltranscribedspacer2todiscriminateH.niger.OurresultsshowthatBar-HRMsuccessfullyidentifiedtheadulterantsanddetectedtheproportionofH.nigerDNAextractwithinanadmixture.Inparticular,HRMdetectedH.nigerDNAextractinA.complanatusDNAextractatconcentrationsaslowas1%.Inconclusion,theBar-HRMmethoddevelopedinthepresentstudyforauthenticatingH.nigerisrapidandcost-effective.ItcanbeusedinthefuturetoguaranteethepurityofHyoscyamiSemenfortheclinicaluse.

简介：Thestereochemistryoftwo6,9-oxygenbridgedibenzocyclooctadienelignansfromKadsuracoccinea,aredifficulttoseparateandveryunstable.Thepresentstudywasdesignedtodevelopahigh-performanceliquidchromatographyusingcirculardichroismdetectionfortheanalysisofthestereochemistry.Anew6,9-oxygenbridgedibenzocyclooctadienelignansnamedKadsulignanQwasfirstlyfoundwithanS-biphenylconfiguration.TheothercompoundwasidentifiedasKadsulignanLwithanR-biphenylconfiguration.Inordertoobtainkineticdataontheirreversibleinterconversion,thestabilitywasmeasuredatdifferentdeuteratedsolventssuchasdeuteratedmethanol,deuteratedchloroformanddeuterateddimethylsulfoxide.Thelignansweremoreunstableandconvertedmoreeasilyindeuteratedmethanolthanindeuteratedchloroformanddeuterateddimethylsulfoxide.

简介：Sesquiterpene内酯在他们的特殊结构和活动的优点在Kudiezi注射被看作主要活跃混合物。此处，一个分析方法为用结合的高效的液体层析的在Kudiezi注射的快速的屏蔽和sesquiterpene内酯的鉴定被开发在否定离子模式的线性离子trap-orbitrap团spectrometry(HPLC-LTQ-Orbitrap)。首先，二个sesquiterpene内酯参考标准被分析获得他们的典型ESI-MS/MS破碎模式。第二，基于提取离子层析(EIC)数据挖掘方法和典型破碎小径分析，在Kudiezi注射的sesquiterpene内酯很快被屏蔽并且识别。最后，一个重要参数堵塞P被采用区别sesquiterpene内酯的异构体。作为结果，50sesquiterpene内酯被描绘，包括9sesquiterpene内酯aglycones，39sesquiterpene内酯glycosides，和2氨基的acid-sesquiterpene内酯结合。在他们之中，13混合物尝试性地作为新混合物被识别。结果证明确定的方法将是为在自然的药的复杂系统屏蔽和sesquiterpene内酯的鉴定的一个快速的、有效分析工具。

简介：TocharacterizeandidentifymultipleconstituentsinDanhonginjection(DHI),afastultra-highperformanceliquidchromatographycoupledtoelectrosprayionizationquadrupoletime-of-flighttandemmassspectrometry(UHPLC-ESI-QTOF/MS)methodwasestablishedandvalidatedinthepresentstudy.Atotalof63compounds,including33phenolicacids,2C-glycosylquinochalcones,6flavonoidO-glycosides,4iridoidglycosides,6organicacids,5aminoacids,and3nucleosides,wereidentifiedortentativelycharacterized.Inconclusion,theUHPLC-ESI-QTOF/MSmethodisusefulandefficientforin-depthstructuralelucidationofchemicalcompoundsincomplexmatricesofherbalmedicinessuchasDHI.

简介：Thepresentstudywasdesignedtodevelopandvalidateasensitiveandreliableultrahighperformanceliquidchromatographycoupledwithquadrupoletime-of-flightmassspectrometry(UPLC-QTOF/MS)methodtoseparateandidentifythechemicalconstituentsofQixueShuangbuTincture(QXSBT),aclassictraditionalChinesemedicine(TCM)prescription.UndertheoptimizedUPLCandQTOF/MSconditions,56componentsinQXSBT,includingchalcones,triterpenoids,protopanaxatriol,flavonesandflavanoneswereidentifiedandtentativelycharacterizedwithinarunningtimeof42min.Thecomponentswereidentifiedbycomparingtheretentiontimes,accuratemass,andmassspectrometricfragmentationcharacteristicions,andmatchingempiricalmolecularformulawiththatofthepublishedcompounds.Inconclusion,theestablishedUPLC-QTOF/MSmethodwasreliableforarapididentificationofcomplicatedcomponentsintheTCMprescriptions.