简介：

简介：

简介：AIM:Toinvestigatetheneuroprotectiveeffectofgastrodinonretinalganglioncells(RGCs)inanacuteocularhypertension(AOH)ratmodelandtoidentifyitspossiblemechanism.METHODS:AOHratmodelwasperformedinarandomlyselectedeyebyanteriorchamberperfusionandeitherreceivedanintraperitonealinjectionwithvariousconcentrationsofgastrodinornormalsaline.After2wk,theratsweresacrificed.FluoroGoldwasusedtolabelsurvivalRGCs.Immunostainingwithanti-Iba1intheretinalflatmountstocalculatethemicrogliadensityintheganglioncelllayer(GCL).Changesinmicroglialcytokines,tumournecrosisfactor-alpha(TNF-α)andinducibleNOsynthase(iNOS)wereexaminedwithWesternblotandreversetranscriptionquantitativepolymerasechainreaction.Expressionlevelsoftotalandphosphorylatedp38mitogenactivatedproteinkinase(MAPK)weredeterminedbyWesternblot.RESULTS:ResultsshowedthatAOHinducedsignificantlossofRGCsandseveremicrogliaactivationintheGCL.Besides,AOHincreasedthephosphorylationofp38MAPKandpromotedthereleaseofmicroglialcytokinesintheretinas.Intraperitonealinjectionwithdose-dependentgastrodinsignificantlyreducedthelossofRGCsandinhibitedretinalmicrogliaactivation,accompaniedwiththedecreasedexpressionlevelsofmicroglialcytokinesandp38MAPKphosphorylation.CONCLUSION:GastrodinexertsaneuroprotectiveeffectonRGCsinanacuteglaucomaanimalmodelviainhibitingmicrogliaactivationandmicroglial-mediatedneuroinflammation.ThefindingdemonstratesthepotentialapplicationofgastrodinintheneuroprotectivetherapyofacuteglaucomaandotherretinalneurodegenerativediseasescharacterizedbymicrogliaactivationandRGCsdeath.