简介：Theestablishmentofsuspensionculturesystemforneem(AzadirachtaindicaA.Juss)cellsandthesuspensioncultureconditionwasstudied.ItshowsthattheneemcellsuspensionculturesystemwasbestinB5liquidmedium,2.0~4.0mg/LNAAwithdirectspillmethod.Basedontheintegratedanalysisofcellbiomass,Azadirachtincontentandproductivity,theoptimumcultureconditionswereB5liquidmedium,2.0-4.0mg/LNAA,3%sucroseat25℃.Theoptimumrotatingspeedoftheshakerandbrothcontentd...

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简介：MultiplicationofElaeagnusangustifoliaL.wasexaminedinvitrosuccessivelyfromasingleshootunderthespecifiedconditionofdifferentmedia,plantgrowthregulators,pHvalueandsucroseconcentration.ItwasshowedthatMMS1wasthemostsuitablemediumonshootmultiplicationamong5mediaconcerned;BAPwasthemosteffectiveoneamongallthecytokinininvolved,BAP,KN,TDZandZT;theexpluntofthetophalf-partfromashootproducedmorenewshootsthanthatofthefoothalf-partdid;morenewshoots(>2cm)wereproducedunder3%sucrosebetweentheconcentrationswithtophalf-partexplants;shootscouldgrowwellbetweenpH4.4andpH7.0,andthebiggestnumberofshootswasproducedinpH5.6,whileinpH5.8themaximumrootingrateappeared.Asaresult,thecombinationof0.5μMBAPand0.1μMIBAonMMS1mediuminducedthemaximumshootmultiplication.Thenumberofshootamplified3timesin1month,and3^12shoots(>2cm)mightbetheoreticallymultipliedannuallyfromasingleshoot.

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简介：Theanatomycharacteristicsofcellwallandlignindistributionindifferentanatomicalregionsfor12-month-oldbamboospeciesPseudosasaamabiliswasinvestigated.Thelignindistributionwasstudiedbymeansofopticalmicroscopeandconfocallaserscanningmicroscopeandthecolorreaction.Inaddition,thedistributionofligninindifferenttissues(fiber,parenchymaandvessel)wasinvestigatedbyvisible-lightmicrospectrophotometrycoupledwiththeWiesnerandMaulereaction.Thecellwallsofdifferenttissueswerealllignifiedandtheligninwaswidelydistributedindifferenttissues,inwhichtherewasadifferencebetweentissuesandanatomicalregionsinlignincontent.Guaiacylligninandsyringylligninunitcouldbefoundincellwalloffiber,parenchymaandvesselthroughWiesnerandMaulereactionandtheabsorbancepeakofspectrum.Therewasnoregularvariationinlignincontentwithdifferentradiallocationofculmsanddifferentlocationinfiberstrands.Thelamellationofthefibercellwallwasgenerallyhadasalternatingbroadandnarrowlayerswithalternatinglowandhighconcentrationoflignin.

简介：用修改了的异硫氰酸胍法从两种森林昆虫Closteraanastomosis和Saperdapopulnea的不同组织中快速提取总RNA.电泳检测和cDNA合成分析结果表明,RNA未降解.经紫外分光光度计检测,A260/A280在1.8到2.0之间,表明RNA的纯度很高.用RT-PCR合成的双链cDNA的长度大于2kb,表明mRNA的完整性.用PCR的方法克隆了C.anastomosisβ肌动蛋白和几丁质酶的基因片段,表明RNA可用于其它分子操作.使用这个方法,在4个小时内可至少从8个样品中提取RNA并进行电泳分析.这些结果表明用这个修改后的一步法提取昆虫总RNA是省时,省钱和有效的.

简介：解剖特征为提取气候的信号被证明有用。为了检验气候的信号，在Changbai山由树戒指房间特征记录了，我们测量了房间数字和房间腔直径除了戒指宽度，朝鲜的松(Pinuskoraiensis)，树在地点响改变了举起，并且我们开发了房间数字(CN)的年表，吝啬的腔直径(MLD)，最大的腔直径(MAXLD)和树戒指宽度(TRW)。年表与气候的因素月刊平均数温度和降水的和被相关。是由我们的分析出现，房间参数年表对dendroclimatology研究合适。CN和TRW分享了不同于MLD和MAXLD的相对类似的气候的信号，并且生长气候关系是举起依赖者，作为由下列调查结果出现：(1)在记录的每举起，MLD和MAXLD，从那些的不同每月气候的信号为一样的气候的因素由TRW记录了；并且(2)MLD和MAXLD记录了不在在更低、中间的举起的TRW的气候的因素。房间腔直径证明了是为为这个学习区域改进气候重建的一个有效档案。