简介：ObjectiveToestablishalymphocytelinecapableoflongsurvivalandexpressinghumanNT-3tolayafoundationforfutureanimalandhumancochleargenetransfectionresearch.MethodsWecollectedlymphocytesfromnormalhumanbloodviaFicollfluidandaddedIL-2intotheserumculturemediumtopromotelymphocytegrowth.TheNT3cDNAwasobtainedbyRT-PCRandligatedwiththeeukaryonvectorwhichispIRES-DsRed2usingT4DNAenzyme.TheNT3cDNAgenewastransfectedintothelymphocytelineusingcationicliposome(LP2000).ThelymphocytestransfectedwithNT3-cDNAwereexaminedbyRT-PCRandWestern-blotmethods.ResultsWeestablishedanewmethodtoextendinvitrolymphocytessurvivaltimeandtotransfectNT3intolymphocytes.Thegeneticallyengineeredlymphocyteswerecapableofsurvivingoverrelativelylongtime.PositiveproteinsignalswereobtainedbyWesternblot.ConclusionsUsinglymphocytesastheintermediary,recombinedplasmidpIRES-DsRed2NT3isusedtoestablishalymphocytelinethatexpressesandsecretesNT3.Thiscelllinecanbeusedinfutureanimalgenecochleartransfectionresearchandmayhelpfindanintermediarycelllineforgenetherapyforhumandeafness.

简介：HumanprimaryepithelialcellsofrenalpelviswasestablishedtoinvestigatetheadherenceofuropathogenicEscherichiacoli(UPEC)tothiscellline,inwhichtheprimarycellculturewasperformedbyusingcultivationofthenormalepitheliumofrenalpelvisinkeratinocyteserumfreemedium(K-SFM)withepidermalgrowthfactor(EGF)andbovinepituitaryextract(BPE).BothUPEC132obtainedfromurinespecimenofpatientswithpyelonephritisandthepilus-freerepresentativestrainE.coliK-12p678-54wereusedtostudytheadherenceofthesestrainsonhumanprimaryepithelialcellsofrenalpelvis.TheUPECadherencewasperformedwithobservationonthemorphologicalchangesoftheadheredcells,whiletheadhesionratesandindiceswerecalculatedindifferenttimesofexperiment.Inaddition,thevirulencegeneshlyandcnf1ofUPEC132weredetectedbymultiplexPCRassay.Inthisstudy,thehumanprimaryepithelialcellsofrenalpelviswasfoundtoexhibitthecharacterofthetransitionalepithelialcells.Comparedwiththecontrolgroup,theadhesionratesandindicesbegantoincreasefrom15minoftheexperimenttimeandreacheditspeakin120min.TheadhesionrateandindexofUPEC132tohumanprimaryepithelialcellsofrenalpelviswere74.4%and34.0respectively.ManymicroscopicchangesintheprimarycellsadheredwithUPEC132couldbedetected,suchasroundingorirregularityinshape,unevennessinstainingandthecytoplasmicandnuclearchanges.ItsuggeststhathumanprimaryepithelialcellsofrenalpelviscanbeusedfortheexperimentonUPECadhesion,thusprovidingabasisforthefurtherstudyonthepathogenesisofUPEC.

简介：ＩＭＭＵＮＯＣＹＴＯＣＨＥＭＩＣＡＬＣＨＡＲＡＣＴＥＲＩＺＡＴＩＯＮＯＦＣＤ４４ＭＯＬＥＣＵＬＥＳＥＸＰＲＥＳＳＥＤＩＮＨＵＭＡＮＢＲＡＩＮＭＥＴＡＳＴＡＳＥＳＬｉＨｏｎｇ李宏；ＬｉｕＪｉａ刘佳；ＭａｒｔｉｎＨｏｆｍａｎｎ；Ｍａｒｉａｅ－Ｆｒａｎｃｅ...

简介：ＬＩＧＨＴＳＣＡＴＴＥＲＩＮＧＰＲＯＰＥＲＴＹＯＦＨＵＭＡＮＢＬＯＯＤＡＴＴＨＥＷＡＶＥＬＥＮＧＴＨＯＦ８１０ＮＭＬＩＧＨＴＳＣＡＴＴＥＲＩＮＧＰＲＯＰＥＲＴＹＯＦＨＵＭＡＮＢＬＯＯＤＡＴＴＨＥＷＡＶＥＬＥＮＧＴＨＯＦ８１０ＮＭＪｉａｎＺｈｏｎｇ；Ｄ...

简介：ＰＲＯＭＯＴＩＯＮＯＦＩＮＶＩＴＲＯＧＲＯＷＴＨＯＦＨＵＭＡＮＭＥＤＵＬＬＯＢＬＡＳＴＯＭＡＣＥＬＬＳＢＹＥＸＯＧＥＮＥＯＵＳＩＬ－６ＬｉｕＪｉａｉ刘佳；ＬｉＨｏｎｇ李宏；ＨａｍｏｕＭａｒｉｅ－Ｆｒａｎｃｅ；ＮｉｃｏｌａｓｄｅＴｒｉｂｏｌｅｔ（１Ｄｉ...

简介：ObjectToexploretheproceduresinper-operativeharvestingandmanagementoffreshhumancochlearspecimensforresearch.MethodsDuringtrans-cochlearsurgerytoremovelargepetro-clivalmeningiomascausinglife-threateningcompressiononthebrainstem,cochleaearenormallydestroyedanddrilledawayinordertoreachtheapicalpetrousandclivusregion.Insteadthecochleacanbedissectedoutafterethicalper-missionwasobtainedfromthelocalethicalcommittee(EPN)andallowancegainedfromthepatients.Sur-geryisperformedbyateamconsistingofoto-andneurosurgeonsasatwo-dayprocedurewithtotalpetro-sectomyincombinationwithaninferiorre-routingofthefacialnerve.Fixationofthecochleaewasdoneintheoperatingroomassoonasthespecimenshadbeenseparatedfromthetemporalbones.Decalcificationbeganafterhours’toovernight’sfixationfor4weeks.Sectioningparalleltothemodiolus(mid-modiolus)wasperformedwithacryostatmicrotome.Thesectionsweresubjectedtoimmunofluorescence(IF).ResultsUsingfreshlyprepared4%paraformaldehyde(PFD)solution,adequatefixationoffineinnerearstructureswasachievedwithhours’immersionofthecochlearspecimens.Decalcificationin6.2%ethylenedi-amine-tetraceticacid(EDTA)solutionfor4weeksyieldedathoroughlydecalcifiedcochlea.Experiencesinprocessing14humancochleaeandanalysingmainlandmarksinfivehumaninnerearplastic/siliconecastsshowedthattheovalwindow/stapesfootplatearebackwardtilted,atanangleabout15degrees,fromtheplaneperpendiculartothemodiolaraxis.Thedistancefromthemodiolarapextotheanteriorborderoftheovalwindow/footplateintheseinnerearcastsmeasuredbetween4and5mm.HighqualityIFstainingwasobtained.ConclusionSurgicallyobtainedhumancochlearspecimen,whenproperlyprocessed,containside-allypreservedantigenicityforimmunohistochemicalstudy.Adequateorientationduringsectioninghelpsob-tainoptimalmid-modiolarsectionsshowingfi

简介：TheproteomicsofthedifferentialproteinexpressionsinhumangliomacelllineU251cellsinfectedwithhumancytomegalovirus(HCMV)wasinvestigatedattheproteinlevelbyusingthesurfaceenhancedlaserdesorption/ionization(SELDI)proteinchipsysteminordertodevelopamethodofstudyforthepathogenesisofHCMVinfection.Inthisstudy,theculturedU251cellswereinfectedwithHC-MVingoodconditionandthesupernatantsoflysatesandtheextracellularfluidsofthecultivatedinfect-edcellswerequantitativelydefinedfortheexpressedproteins.TheproteomicsofthedifferentialproteinexpressionincellsbeforeandafterinfectionwasanalyzedbyWCX2arraysontheproteinchipreader.Itwasdemonstratedthatthecytopathiceffectsofinfectedcellsappearedonthe5thdayafterinfection,however,thedifferentialproteinexpressionwasevidentat6hafterinfectionasrevealedbyRT-PCRandmassspectrometry.Theproteinpeakscapturedfromdifferentbatchesofsamples,fromthesamesampledetectedwithdifferentarraysorforthedifferentlimeswereallequivalent.Withthemolecularweightrangefrom2000Dato3000Da,chipcaptured82peaksfromtheintracellularfluidsand11proteinpeakfromthecellularfluidinwhichcomparedwiththecontrolgroup,theproteinpeakswithmolecularweightof13536.3Da,10046.1Daand17106.2Dawereclosetothoseofβ-amyloidpro-tein,caspase-1precursorandLPS-inducedTNF-αfactorrespectively,whichshowedbriefup-regulation4hafterinfection,andcontinuedtoraise48hlater.Theseresultsinferthattheseproteinsmaybere-latedtotheapoptosisinducedbyHCMVinfection,thussuggestingthattheapeptosisinducedbyHC-MVinfectionmayplayaroleinthepathogenesisofHCMVinfection.

简介：ＡＮＴＩＣＯＭＰＬＥＭＥＮＴＡＲＹＡＣＴＩＶＩＴＹＩＮＨＵＭＡＮＳＥＲＵＭＯＦＬＵＮＧＣＡＮＣＥＲＰＡＴＩＥＮＴＳＡＮＤＩＴＳＰＯＳＳＩＢＬＥＣＬＩＮＩＣＡＬＳＩＧＮＩＦＩＣＡＮＣＥＬｉｕＨｕｉｒｏｎｇ刘慧荣ＬｉａｎｇＦｅｎｇ梁峰ＺｈａｎｇＷｅｉｆ...

简介：AbstractBackground:Cervical cancer remains a major public health issue for the Uyghur women and other women living mainly in rural areas of Xinjiang. This study aims to investigate the distribution of human papillomavirus (HPV) infection and cervical cancer in rural areas of Xinjiang, China.Methods:Cervical cancer screening was performed on rural women aged 35 to 64 years from Xinjiang, China in 2017 through gynecological examination, vaginal discharge smear microscopy, cytology, and HPV testing. If necessary, colposcopy and biopsy were performed on women with suspicious or abnormal screening results.Results:Of the 216,754 women screened, 15,518 received HPV testing. The HPV-positive rate was 6.75% (1047/15,518). Compared with the age 35-44 years group, the odds ratios (ORs) of HPV positivity in the age 45-54 years and 55-64 years groups were 1.18 (95% confidence interval [CI]: 1.02-1.37) and 1.84 (95% CI: 1.53-2.21), respectively. Compared with women with primary or lower education level, the ORs for HPV infection rates of women with high school and college education or above were 1.37 (95% CI: 1.09-1.72) and 1.62 (95% CI: 1.23-2.12), respectively. Uyghur women were less likely to have HPV infection than Han women, with an OR (95% CI) of 0.78 (0.61-0.99). The most prevalent HPV types among Xinjiang women were HPV 16 (24.00%), HPV 33 (12.70%), and HPV 52 (11.80%). The detection rate of cervical intraepithelial neoplasia (CIN)2+ was 0.14% and the early diagnosis rate of cervical cancer was 85.91%. The detection rates of vaginitis and cervicitis were 19.28% and 21.32%, respectively.Conclusions:The HPV infection rate in Xinjiang is low, but the detection rate of cervical cancer and precancerous lesions is higher than the national average level. Cervical cancer is a prominent public health problem in Xinjiang, especially in southern Xinjiang.

简介：AbstractBackground:In recent years, a programme of vector control, screening and treatment of gambiense human African trypanosomiasis (gHAT) infections led to a rapid decline in cases in the Mandoul focus of Chad. To represent the biology of transmission between humans and tsetse, we previously developed a mechanistic transmission model, fitted to data between 2000 and 2013 which suggested that transmission was interrupted by 2015. The present study outlines refinements to the model to: (1) Assess whether elimination of transmission has already been achieved despite low-level case reporting; (2) quantify the role of intensified interventions in transmission reduction; and (3) predict the trajectory of gHAT in Mandoul for the next decade under different strategies.Method:Our previous gHAT transmission model for Mandoul was updated using human case data (2000-2019) and a series of model refinements. These include how diagnostic specificity is incorporated into the model and improvements to the fitting method (increased variance in observed case reporting and how underreporting and improvements to passive screening are captured). A side-by-side comparison of fitting to case data was performed between the models.Results:We estimated that passive detection rates have increased due to improvements in diagnostic availability in fixed health facilities since 2015, by 2.1-fold for stage 1 detection, and 1.5-fold for stage 2. We find that whilst the diagnostic algorithm for active screening is estimated to be highly specific (95% credible interval (CI):99.9-100%, Specificity = 99.9%), the high screening and low infection levels mean that some recently reported cases with no parasitological confirmation might be false positives. We also find that the focus-wide tsetse reduction estimated through model fitting (95% CI :96.1-99.6%, Reduction = 99.1%) is comparable to the reduction previously measured by the decline in tsetse catches from monitoring traps. In line with previous results, the model suggests that transmission was interrupted in 2015 due to intensified interventions.Conclusions:We recommend that additional confirmatory testing is performed in Mandoul to ensure the endgame can be carefully monitored. More specific measurement of cases, would better inform when it is safe to stop active screening and vector control, provided there is a strong passive surveillance system in place.

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简介：ToscreenandidentifytheshortpeptideswithspecificbindingactivitytohumanCDS9andtodesigntheshort-peptideclampagainsttumorescape,thephagedisplaypeptidelibrarycontaining12peptideswasusedtoselectthehighlyexpressedspecificcoalescentpeptideofhumanCD59inCHOcells.Positivephageclonesobtainedafter5roundsofbiopanninganddetectedwithELISAwereobtained,inwhich8ofthemwithhighbindingactivitytohumanCD59weresequenced.The3sequencesthusobtainedshowedhighhomologywitheachandcertainhomologywithsequencewithhumanCD2(PubMed339HGAAENSISPSS),andallcontainedprimarystructureHXAXXXXXXPXX,ofwhichthissequencemaybethemimicconformationalepitopebindingtohumanCD59.Theseresultsinthepresentstudymaybehelpfultodesigntheshort-peptideclampagainsttheactivesitesofCD59ontumorescape.

简介：Osteochondrosisdissecans(OD)ofthehumankneeisanasepticprocessofsub-chondralbonenecrosiswhichoftenleadstothepartialorcompleteseparationofasubchondralfreeparticlecoveredwitharticularcartilage.Epidemiologicalstudieshaverevealedthatincidentalpeakofageconcentrateintheseconddecadeoflife,es-peciallyinhighlyexercisedpopulationsuchasathletes.Thelocationoftheinvolved

简介：颈的spondylosis的.The发生率高，并且由于颈的椎骨的复杂性组织形状的不规则和部件的不一致，有时通过应力和紧张分析在vitro由实验完成计划目的是困难的。而且，biomechanical因素具有在针的混乱的原因的重要意义。在这篇论文，作者做人的颈的椎骨的在场建模的一篇摘要并且描述通过几个自我构造的模型建立人的颈的椎骨的有限元素模型的主要方法。随着计算机技术的前进，有限元素方法很快在颈的椎骨biomechanical研究被开发了并且有成为了一条主要途径让biomechanical研究模仿越来越临床的条件。

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简介：EnhancingSchwanncellproliferationmaybebeneficialforperipheralnerverepairandnerveregeneration.AtraditionalherbalformulacomposedofFuling(poriacocos),Baizhu(Atractylodesmacrocephala),andDanggui(Angelicasinensis)(FBD)improvesneuronalsurvivalandgrowth,andFBDmaypromotethesecretionofbrain-derivedneurotrophicfactor.However,themechanismunderlyingSchwanncellproliferationremainsunclear.WetestedwhetherFBDenhancedtheproliferationofhumanSchwanncells.FBD(20μg/mL)increasedSchwanncellviabilityandsurvival,andincreasedthenumberofcellsatG2/MandSphases.FBDalsoincreasednervegrowthfactorandbrain-derivedneurotrophicfactorexpressioninSchwanncells,withmaximumefficacyat20μg/mL.

简介：Objective:Livermetastasis,whichcontributessubstantiallytohighmortality,isthemostcommonrecurrentmodeofcoloncarcinoma.Thus,itisnecessarytoidentifygenesimplicatedinmetastaticcolonizationoftheliverincoloncarcinoma.Methods:WecomparedmRNAprofilingin18normalcolonmucosa(N),20primarytumors(T)and19livermetastases(M)samplesfromthedatasetGSE49355andGSE62321ofGeneExpressionOmnibus(GEO)database.Geneontology(GO)andpathwaysoftheidentifiedgeneswereanalyzed.Co-expressionnetworkandproteinproteininteraction(PPI)networkwereemployedtoidentifytheinteractionrelationship.SurvivalanalysesbasedonTheCancerGenomeAtlas(TCGA)databasewereusedtofurtherscreening.Then,thecandidategeneswerevalidatedbyourdata.Results:Weidentified22specificgenesrelatedtolivermetastasisandtheywerestronglyassociatedwithcellmigration,adhesion,proliferationandimmuneresponse.Simultaneously,theresultsshowedthatC-X-Cmotifchemokineligand14(CXCL14)mightbeafavorablepredictionfactorforsurvivalofpatientswithcoloncarcinoma.Importantly,ourvalidateddatafurthersuggestedthatlowerCXCL14representedpooreroutcomeandcontributedtometastasis.Genesetenrichmentanalysis(GSEA)showedthatCXCL14wasnegativelyrelatedtotheregulationofstemcellproliferationandepithelialtomesenchymaltransition(EMT).Conclusions:CXCL14wasidentifiedasacrucialanti-metastasisregulatorofcoloncarcinomaforthefirsttime,andmightprovidenoveltherapeuticstrategiesforcoloncarcinomapatientstoimproveprognosisandpreventmetastasis.

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