简介:TheaimofthisstudyistoexplorethegenomicmolecularorganizationandgenogroupofhumannorovirusfrominfectedinfantsinGuangzhouofChina.PrimersweredesignedaccordingtothegenomicsequenceofnorovirusintheGenBank,andthenorovirusgenomewasamplifiedbyRT-PCR.ThePCR-productswereclonedintoTvectorandsequenced,andthegenomicnucleotidesequenceswereanalyzedwiththeprogramsCLUSTALW/X,DNASTARandRAT(RecombinationAnalysisTool).TheNVgz01straingenomeis7558bpinlengthandencodesthreeopenreadingframes(GenBankaccessionNo.isDQ369797).ThegenomicsequencesofNVgz01werecomparedwiththoseofnomvirusinGenBank,whichrevealedthatthehomologywithgenogroupⅡrangesbetween76%-90%,andgenogroupⅠbe-tween43%-44%.TheORF1regionshared94%and88%identitywithMc37andFarmingtonstrains,respectively;thecapsidregion(ORF2)shared65%and94%identitywithMc37andFarmingtonstrains,respectively.Phylogenetictreeswerereconstructedbytheneighbor-joiningmethod.ComparativecompletesequenceanalysisoftheNVgz01withreportedhumannorovimsgenomicsequencesrevealedthatthisisolatebelongstogenogmupⅡ.TheORF1andORF2regionsshareddifferentidentitywithMc37andFarmingtonstrains,suggestingNVgz01couldbearecombinantvires.
简介:摘要目的探讨产道血肿形成的原因和处理方法。结果主要症状为会阴部疼痛、坠胀,见于阴道两侧沟、会阴侧切处及其深部。多见于初产妇。以产后4小时特别是产后2小时即可发现并有明显症状。结论为预防产道血肿的形成,要加强产后观察,认真细致地进行阴道缝合。
简介:人的一生就像花的历程,有雨雪,也有风霜,经历过了,才会有美丽的绽放。那个实习医生刚刚离去。我静静地躺在床上,透过巨大的玻璃窗望去,美丽的椰子树后是湛蓝高远的天空。在屋内,我能清晰地听到潮水轻抚沙滩的细切的声音,就像烛光中妈妈的催眠曲一样亲切温暖。
简介:宫颈肌瘤是位于子宫颈部的良性肿瘤,发病率较低,较宫体部肌瘤在诊断和处理中有其一定的特殊性。本文对此进行分析。1临床资料1.1一般资料:我院自1978年4月~1999年6月期间,收治子宫肌瘤377例,其中宫颈肌瘤13例,占3.45%。发病年龄为24~54岁,平均为41.2岁;其中32岁~50岁10例,占76.92%。其中,合并宫体肌瘤4例,子宫腺肌瘤3例,卵巢肿瘤1例。所有标本均经病理检查证实。1.2分类:按其生长部位分二类:即宫颈阴道段5例和宫颈上段8例。按瘤体与肌壁的关系可分三类:肌壁间肌瘤7例(占53.85%);浆膜下肌瘤,突入阔韧带内4例(占30.77%);粘膜下肌瘤,肌瘤突入宫颈管内堵住宫口,或排出宫口2例(占15.38%)。1.3临床表现:13例中有月经过多6例,普查中发现或患者本人察觉肿块者4例,排尿困难、腰腹酸痛及白带增多3例。1.4术中所见:子宫形态,除合并有宫体肌瘤外,见子宫体正常大小或略大,宫颈拉长膨大,使子宫呈“亚铃状”,宫颈展平挤压而难以辨认。肌瘤最大:17cm×15cm×10cm。最小:
简介:Supernova(SN)neutrinosdetectedontheEartharesubjecttotheshockwaveeffects,theMikheyev–Smirnov–Wolfenstein(MSW)effects,theneutrinocollectiveeffectsandtheEarthmattereffects.Consideringtherecentexperimentalresultaboutthelargemixingangleθ13(■8.8°)providedbytheDayaBayCollaborationandapplyingtheavailableknowledgefortheneutrinoconversionprobabilityinthehighresonanceregionofSN,PH,whichisintheformofhypergeometricfunctioninthecaseoflargeθ13,wededucetheexpressionofPHtakingintoaccounttheshockwaveeffects.ItisfoundthatPHisnotzeroinacertainrangeoftimeduetotheshockwaveeffects.Afterconsideringallthefourphysicaleffectsandscanningrelevantparameters,wecalculatetheeventnumbersofSNneutrinosforthe"Garching"distributionofneutrinoenergyspectrum.Fromthenumericalresults,itisfoundthatthebehaviorsofneutrinoeventnumbersdetectedontheEarthdependontheneutrinomasshierarchyandneutrinospectrumparametersincludingthedimensionlesspinchingparameterβα(whereαreferstoneutrinoflavor),theaverageenergy