简介：InordertoobserveseveralantibodiestoliverantigensinChinesepatientswithdifferentfiverdiseasesandtodiscussthecharacteristicsoftheautoantibodiesinautoimmuneliverdiseases,from1412patients,detectedbyindirectimmumofluoreseence(IIF)initially,230patientswithabnormalALTwerechosenanddividedinto5groups:①autoimmunediseasesgroup,42cases:18withautoimmtmehepatitis(AIH),21withprimarybiliarycirrhosis(PBC),3withprimarysclerosingcholangitis(PSC).②HAVgroup,23cases;③HBVgroup,70cases;④HCVgroup,35casesand⑤NonA-Egroup,60cases.First,ANA,AMA,SMA,liver-kidneymicrosomalantibody(LKM)andsoonweretestedby1/F.Then,LKM-1,fivercytosofic-1(LC-1),solubleliverantigen/fiverpancreas(SLA/LP)andsubtypeofAMA(M2)aswellasANAprofilesuchasSS-A,SS-BanddsDNAweretestedbyWesternblotandimmtmoblotstripsassay,respectively.Theresultswerethatamong1412cases,thosediagnosedasAIH,PBCandPSCaccotmtedfor12.7‰,14.9‰and2.1‰,respectively,ofthesamplesbeingtested.2/230withLKM-1and2/230withSLA/LPwereseeninindividualsinfectedwithAIHandHCV,respectively.AllpatientswithPBCshowedAMAandM2antibodies.NospecificANApatternwasseeninAIHby1/Fbutanti-actinwasonlyfoundinpatientswithAIH.InNonA-Egroup,fourcaseswerepositiveofAMAandM2;threehadhightiterofSMAandother4hadSS-A,SS-BordsDNAantibodies,etc.Itwasconcludedthatthedetectionofanti-fiverantigens,ANAprofileandAMAsubtypeswerehelpfulforthediagnosisofautoimmunefiverdiseasesandoverlapsyndromes.InpatientswithNonA-Ehepatitis,thediagnosisofPBCorAIHshouldbetakenintoconsideration.

简介：Highlevelsoflowmolecularweight(LMW)IgMincertaindiseasesareassociatedwithclinicalandlaboratoryindiceswhichreflecttheseverityofthedisease.TheseassociationssuggestthatLMWIgMmayplayanimportantroleintheimmunopathogenesisofthesediseases.TofurtherapproachthequestionconcerningthefunctionalactivityofLMWIgMindisease,apanelofLMWIgMandhighmolecularweight(HMW)IgMpreparationswithorwithoutrheumatoidfactor(RF)activitywereusedtoinvestigatetheirantibodybindingactivityandtheireffectorfunction.ItwasfoundthatLMWIgM-RFandHMWIgM-RFhadasimilarbindingcapacitytoFcfragmentastherewasnosignificantdifferenceintheaffinityindexbetweenthem.ItfurthershowedthattherateofactivationandtotalamountofutilizationofcomplementbyLMWIgMandHMWIgMwassimilar,althoughthemeanfluorescenceofC3depositionbyIgM-RFandHMWIgM-RFwasslightlyhigherthanthatofLMWIgM-RFandothercontrolRFantibodies.However,thecurrentstudydemonstratedthatLMWIgMhadstrongneutrophilactivatingpropertieswhencomparedwithHMWIgM.ThesefindingssuggestthatonemechanismofLMWIgMcontributingtotheimmunopathogenesisofRAmaybeduetotheformationofcirculatingimmunecomplex(CIC)byLMWIgMwithsubsequentactivationofneutrophils.WhetherLMWIgMhasotherfunctionalactivityindiseaseisunclearandneedsfurtherinvestigation.

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简介：TheaimofthisstudyistofurtherunderstandthegenotypeofHantavirus(HV)fromperipheralbloodofpatientswithhemorrhagicfeverwithrenalsyndrome(HFRS)andtheepidemiologicalsignificanceofthisdiseaseinHeilongjiangprovinceinrecentyears.Thirty-oneserumsamplesofclinicallydiagnosedpatientswithHFRSwereexaminedbyRT-PCRtodecidethegeneticsubtype.Onthebasisofinfectionseason,theserumsamplesweredividedintotwogroups:winter(Nov,2003—Feb,2004),springandsummer(April,2004—Sep,2004).Furtheranalysiswasperformedincombinationwithclinicalsymptoms.Itwasfoundthatamongthetotal31samples,22weresero-positive.Among14serumsamplesinwinter,8weresero-positive,ofwhich5caseswereoftypeⅠ(Hantaanvirus,HTNV)and3oftypeⅡ(Seoulvirus,SEOV).Among17samplesinspringandsummer,14weresero-positive,ofwhich5caseswereoftypeⅠand9oftypeⅡ.SoitconcludesthatbothofthetwotypesofHantavinisexistinHeilongjiang.ThetypeⅠisthemainpathogenofHFRSinwinter,andtypeⅡisthemaininspringandsummer.

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简介：TheaimofthepresentstudywastodeterminetheefficacyofimmunotherapywithdendriticcellstoelicitEBV-specificCTL-immunityinadvancedcasesofEBV-positivepatientswithnasopharyngealcarcinoma(NPC)andtodeterminethesafetyandtoxicityofthispreparation.NinecasesofhistologicallyconfirmedpatientswithNPCundergoingtreatmentwithradiologicaltherapywereenrolledinthisstudy.Dendriticcells,generatedinvitrofrombloodmonocytesofpatientswereculturedandmaturedwithcytokinesandtheninfectedwithrecombinantadenovirusvaccinecontainingEBV-latentmembraneprotein-2(Ad-LMP2).On9days'cultivationofcells,thematuredDCswereharvested,irradiatedwithCoandtheninjectedintradermallytopatientswithNPC.Theinjectionswereperformed3timestotally.Afterimmunization,theCTLresponseswereassayedbymeansofcytotoxicityandepitope-specificIFN-γproduction.Theresultsofthistrialshowedthatallpatientscouldtoleratethiskindoftreatmentwithoutanysideeffect,duringwhichmarkedincreaseofLMP2-specificCTL-responsescouldbedemonstratedin5patientsofthisgroup.AndthelevelofIgA/VCAantibodydecreasedin8of9patients,thusaccountingforabetterprognosisforthesepatients.Allpatientswillbefollowedupforanotheroneyear.Atleast,thepresentworkshowsthatintradermalvaccinationwithautologousDCsinfectedwithrecombinantAd-LMP2adenovirusisasafeprocedureinNPCpatients,inwhichthisprocedurecanenhancetheLMP2-specificCTLresponsesinpatients.Thesedataareencouragingtodevelopmoreeffectivevaccinestrategiesforthetreatmentofnasopharyngealcarcinoma.

简介：Wereportedanovelmammalianreovirus,designedBYD1,isolatedfromthroatswabsofpatientswithsevereacuterespiratorysyndrome(SARS),in2003.Inthepresentstudy,wefirstlycomparedthegenomeelectrophoreticmigrationpatternsofreovirusBYD1with3prototypereovirusstrainsbypolyacrylamidegelelectrophoresis(PAGE)anddeterminedthecompletenucleotidesequenceoftheS1genesegmentofBYD1bysingleprimeramplificationtechnique.TheelectropherogramofBYD1wasdifferentfromthoseofthe3prototypestrainsandanyotherreovirusisolatesreportedbefore.TheentireS1segmentsequenceofBYD1is1437bplongwithtwomeaningfulopenreadingframes(ORFs).ThelongestORFencodesσ1,thecellattachmentprotein,andthesecondlongestORFsupposedlyencodesσ1s,animportantnonstructuralvirulencefactor.TheterminalsequencesofS1segmentare5'GCUAand3'UCAUC,whichareconsistentwiththoseofothermammalianreoviruses.Thehighesthomologyofdeducedσ1aminoacidsequenceis64%identitywithknownmammalianreoviruses.PhylogeneticanalysisofbothS1nucleotidesequenceandσ1aminoacidsequenceindicatedtheBYD1isolatebelongedtoanewcladeofserotype2group.TheresultsofthisstudyshowedthattheBYD1S1segmentwasmarkedlydifferentfromthoseofisolatesreportedbeforeandBYD1wasanovelhumanreovirusisolate.

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简介：TherelationshipbetweenembBmutationofMycobacteriumtuberculosisandethambutol(EMB)resistanceoftheclinicalisolatesoftuberculouspatientsinChinawasinvestigatedbyreversedotblothybridization(RDBH)inadditiontoevaluatingtheclinicalvaluewithapplicationofPCR-RDBHtechniquetodetectEMBresistance.Inthepresentstudy,thegenotypesofthe258bpfragmentsofembBgenesfrom196clinicalisolatesofM.tuberculosiswereanalysedwithRDBHandDNAsequencing.Itwasdemonstratedthat60outof91phenotypicallyEMB-resistantisolates(65.9%)showed5typesofmissensemutationsatcodon306ofembBgene,resultinginthereplacementoftheMetresidueofthewildtypestrainwithVal,IleorLeuresidues.Inthesemutations,theGTPmutation(38/91,41.8%)andtheATAmutation(16/91,17.6%)werethemostencounteredgenotypes.TheembBmutationatcodon306couldalsobefoundin69isolatesofphenotypicallyEMB-sensitivebutresistanttootheranti-tuberculousdrugs,butnosuchgenemutationcouldbefoundin36strainsofdrug-sensitiveisolates.Meanwhile,theconcordancewiththeresultsofDNAsequencingforonewide-typeprobeand5probesforspecificmutationswas100%.ItwasconcludedthattheEMB-resistanceoccurringinmostM.tuberculosisisduetoappearanceofembBmutationatcodon306,andthePCR-RDBHassaywasprovedtobearapid,simpleandreliablemethodforthedetectionofgenemutations,whichmightbeagoodalternativeforthedrug-resistancescreening.

简介：Inordertoelucidatethemolecularandimmunologicalmechanismsaswellasthepathogenesisofhemorrhagicfeverwithrenalsyndrome(HFRS),theCD8^+cytotoxicTlymphecytes(CTL)clonewasestablisheddirectlyfromperipheralbloodmononuclearcells(PBMC)ofpatientswithHFRS.TheactivitiesofCTLweredetectedasusualwithEBV-transformedlymphoblastoidcellline(BLCL)astargetcells.TheresultsshowedthattheCTLclonecouldrecognizedandkilledthetargetcellswithspecificityofnucleocapsidproteinofHantaanvirus(HTNVNP)withthecytotoxicitypercentagesof50.2%,25.4%and39.0%respectively.TheseresultsdemonstratedthattheantigenicepitopesofHTNVNPmainlylocatedontheC-temainaloftheviralnucleocapsidprotein.

简介：【摘要】 目的 观察颈部血管超声应用于缺血性脑血管病患者的临床价值。方法 选取我院2013年6月—2015年6月诊治的124例缺血性脑血管病发病初期患者为研究组，同期选取124例健康受检者为对照组。两组受检者均在常规检查的基础上进行颈部血管超声检查，观察两组受检者的动脉粥样硬化斑块、颈动脉狭窄、颈动脉中膜厚与脑血流动力学指标等情况。结果 研究组颈动脉与脑血流动力学指标情况均高于对照组，差异有统计学意义(P

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