摘要
Thediscreteexcitation-emission-matrixfluorescencespectra(EEMS)at12excitationwavelengths(400,430,450,460,470,490,500,510,525,550,570,and590nm)andemissionwavelengthsrangingfrom600-750nmweredeterminedfor43phytoplanktonspecies.Atwo-rankfluorescencespectradatabasewasestablishedbywaveletanalysisandafluorometricdiscriminationtechniquefordeterminingphytoplanktonpopulationwasdeveloped.Forlaboratorysimulativelymixedsamples,thesamplesmixedfrom43algalspecies(thealgaeofonedivisionaccountedfor25%,50%,75%,85%,and100%ofthegrossbiomass,respectively),theaveragediscriminationratesatthelevelofdivisionwere65.0%,87.5%,98.6%,99.0%,and99.1%,withaveragerelativecontentsof18.9%,44.5%,68.9%,73.4%,and82.9%,respectively;thesamplesmixedfrom32redtidealgalspecies(thedominantspeciesaccountedfor60%,70%,80%,90%,and100%ofthegrossbiomass,respectively),theaveragecorrectdiscriminationratesofthedominantspeciesatthelevelofgenuswere63.3%,74.2%,78.8%,83.4%,and79.4%,respectively.Forthe81laboratorymixedsampleswiththedominantspeciesaccountingfor75%ofthegrossbiomass(chlorophyll),thediscriminationratesofthedominantspecieswere95.1%and72.8%atthelevelofdivisionandgenus,respectively.Forthe12samplescollectedfromthemesocosmexperimentinMaidaoBayofQingdaoinAugust2007,thedominantspeciesofthe11sampleswererecognizedatthedivisionlevelandthedominantspeciesoffourofthefivesamplesinwhichthedominantspeciesaccountedformorethan80%ofthegrossbiomasswerediscriminatedatthegenuslevel;forthe12samplesobtainedfromJiaozhouBayinAugust2007,thedominantspeciesofallthe12sampleswererecognizedatthedivisionlevel.Thetechniquecanbedirectlyappliedtofluorescencespectrophotometersandtothedevelopingofaninsitualgaefluorescenceauto-analyzerforphytoplanktonpopulation.
出版日期
2012年03月13日（中国期刊网平台首次上网日期，不代表论文的发表时间）
