摘要
Nano-sizedzincoxide(nZnO)particlesareonekindofthemostcommonlyusedmetaloxidenanoparticles(NPs).ThisstudycomparedthecytotoxicandembryotoxiceffectsofthreeincreasingsizedZnOparticles(30nm,80?150nmand2μm)inthefloundergill(FG)cellsandzebrafishembryos,andanalyzedthecontributionofsize,agglomerationandreleasedZn2+tothetoxiceffects.AllthetestedZnOparticleswerefoundtobehighlytoxictobothFGcellsandzebrafishembryos.Theyinducedgrowthinhibition,LDHrelease,morphologicalchangesandapoptosisinFGcellsinaconcentration-,size-andtime-dependentmanner.Moreover,thereleaseofLDHfromtheexposedFGcellsintothemediumoccurredbeforetheobservablemorphologicalchangeshappened.TheultrasonicationtreatmentandadditionofserumfavoredthedispersionofZnOparticlesandalleviatedtheagglomeration,thussignificantlyincreasedthecorrespondingcytotoxicity.ThereleasedZn2+ionsfromZnOparticlesintotheextracellularmediumonlypartiallycontributedtothecytotoxicity.AllthethreesizesofZnOparticlestestedinduceddevelopmentalmalformations,decreaseofhatchingratesandlethalityinzebrafishembryos,butsize-andconcentration-dependenttoxiceffectswerenotsoobviousasinFGcellspossiblyduetotheeasyaggregationofZnOparticlesinfreshwater.Inconclusion,bothFGcellsandzebrafishembryosaresensitivebioassaysystemsforsafetyassessmentofZnOparticlesandtheenvironmentalreleaseofZnOparticlesshouldbecloselymonitoredasfarasthesafetyofaquaticorganismsisconcerned.
出版日期
2017年01月11日（中国期刊网平台首次上网日期，不代表论文的发表时间）
