摘要
Linkerswereassembledonaglasssurfacebasedonthehydrolysisandcondensationof3-glycidoxy-propyltrimethoxysilane(GPS).AftertheassemblyofGPS,fourapproachesweretriedtoopentheendingepoxidegroupofGPSortofurtherelongatethelinkers.TheeffectoftheseapproachesonDNAinsitusynthesisandhybridizationwasinvestigated.Forthespacingofthesynthesisinitiationsites,thewettabilityofthesupportandthelengthofthelinkinggroupthatattachestheinitiationsitetothesurfacehavedirectinfluencesontheyieldofcouplingreactionsandthesubsequenthybridizationevents.X-rayphotoelectronspectroscopy(XPS)andmeancontactanglesofdeionizedwateroftheaboveslidesweremeasuredtoassessthelinker'scharacteristicsineachprocedure.Itwasprovedthattheglassslidesweresuccessfullymodifiedandbecameexcellentsupportsfortheoligonucleotidessynthesis.Inaddition,itprovedbestfortheinsituoligonueleotidessynthesisthataglassslidewasinturntreatedwithethylenediamine,glutaradehyde,ethanolamineandsodiumborohydridesolutionatambienttemperatureaftersilanizedwithGPS.
出版日期
2003年02月12日（中国期刊网平台首次上网日期，不代表论文的发表时间）
