简介：MicroRNAs(miRNAs)areaclassofshort,endogenously-initiatednon-codingRNAsthatpost-transcriptionallycontrolgeneexpressionviaeithertranslationalrepressionormRNAdegradation.ItisbecomingevidentthatmiRNAsareplayingsignificantrolesinregulatorymechanismsoperatinginvariousorganisms,includingdevelopmentaltimingandhost-pathogeninteractionsaswellascelldifferentiation,proliferation,apoptosisandtumorigenesis.Likewise,asaregulatoryelement,miRNAitselfiscoordinativelymodulatedbymultifariouseffectorswhencarryingoutbasicfunctions,suchasSNP,miRNAediting,methylationandcircadianclock.Thismini-reviewsummarizedthecurrentunderstandingofinteractionsbetweenmiRNAsandtheirtargets,includingrecentadvancementsindecipheringtheregulatorymechanismsthatcontrolthebiogenesisandfunctionalityofmiRNAsinvariouscellularprocesses.

简介：生物信息的途径补充了试验性的努力到inventorize植物miRNA目标。我们执行了产生通常认为的miRNA的一张全面的表的transcriptome指向的米饭(Oryzasativa)的全球计算分析。我们的预言(684个唯一的抄本)证明米饭miRNAs调停包括抄写(41%)的多样的功能的规定，催化作用(28%)，绑定(18%)，和transporter活动(11%)。在预言的目标之中，61.7%点击在编码区域，将近72%目标让独居的miRNA撞击。学习预言了通常认为地调整象压力反应，催化作用，和绑定一样的函数的34miRNAs的超过70个新奇目标。非常，目标的一半(55%)在O之间被保存，这被观察。sativaindica和O。sativa装饰用的梨树。31个miRNA家庭的成员被发现拥有在米饭之间并且至少的保存目标另外的草家庭成员之一。大约44%唯一的目标在二个不一样的miRNA预言算法之间是普通的。跨种类的保存和算法的一致的如此的程度在在这研究预言的米饭miRNA目标的表授与信心。

简介：miRNAsarenon-codingsmallRNAsthatinvolvediversebiologicalprocesses.Untilnow,littleisknownabouttheirrolesinplantdroughtresistance.Physcomitrellapatensishighlytoleranttodrought;however,itisnotclearaboutthebasicbiologyofthetraitsthatcontributeP.patensthisimportantcharacter.Inthiswork,wediscovered16droughtstress-associatedmiRNA(DsAmR)familiesinP.patensthroughcomputationalanalysis.DuetothepossiblediscrepancyofexpressionperiodsandtissuedistributionsbetweenpotentialDsAmRsandtheirtargetinggenes,andtheexistenceoffalsepositiveresultsincomputationalidentification,thepredictionresultsshouldbeexaminedwithfurtherexperimentalvalidation.WealsoconstructedanmiRNAco-regulationnetwork,andidentifiedtwonetworkhubs,miR902a-5pandmiR414,whichmayplayimportantrolesinregulatingdrought-resistancetraits.Wedistributedourresultsthroughanonlinedatabasenamedppt-miRBase,whichcanbeaccessedathttp://bioinfor.cnu.edu.cn/ppt_miRBase/index.php.OurmethodsinfindingDsAmRandmiRNAco-regulationnetworkshowedanewdirectionforidentifyingmiRNAfunctions.

简介：WestudiedstructuralandimmunologicalpropertiesoftheSARS-CoVM(mem-brane)protein,basedoncomparativeanalysesofsequencefeatures,phylogeneticinvestigation,andexperimentalresults.TheMproteinispredictedtocontainatriple-spanningtransmembrane(TM)region,asingleN-glycosylationsitenearitsN-terminusthatisintheexteriorofthevirion,andalongC-terminalregionintheinterior.TheMproteinharborsahighersubstitutionrate(0.6%correlatedtoitssize)amongviralopenreadingframes(ORFs)frompublisheddata.ThefoursubstitutionsdetectedintheMprotein,whichcausenon-synonymouschanges,canbeclassifiedintothreetypes.OneofthemresultsinchangesofpI(isoelectricpoint)andcharge,affectingantigenicity.ThesecondchangeshydrophobicityoftheTMregion,andthethirdonerelatestohydrophilicityoftheinteriorstructure.PhylogenetictreebuildingbasedonthevariationsoftheMproteinappearstosupportthenon-humanoriginofSARS-CoV.Toinvestigateitsimmunogenicity,wesynthesizedeightoligopeptidescovering69.2%oftheentireORFandscreenedthembyusingELISA(enzyme-linkedimmunosorbentassay)withserafromSARSpatients.Theresultsconfirmedourpredictionsonantigenicsites.