简介:ThisstudywasaimedtoobservetheexpressionofP70S6kinase(P70S6K)inoralaciniccellcarcinoma.PT0S6kinaseexpressionwasexaminedbymeansofWestern-blottestandActivityas-say.Specimenswerefrom30casesoforalaciniccellcarcinomaand15casesofnormaloraltissuewereusedascontrols.StatisticalanalysissoftwareSPSS10.0wasusedforttesttodeterminetherelationshipbetweengeneexpressionandclinicalfeatures.TheexpressionlevelofP70S6Kincreasedobviouslyinoralaciniccellcarcinomatissue(P<0.01).ActivityassaywasthesameastheWestemblottest(P<0.01).P70S6Kexpressionlevelandactivityplayedanimportantroleinthedevelopmentoforalaciniccellcarcinoma.Inconclusion,P70S6Kisamplifiedandoverexpressedinoralaciniccellcarci-nomatissue,whichsuggestsapotentialoncogenicfunction.P70S6KandotherpossibletargetsofmTORcontributesignificantlytotumordevelopmentandthatinhibitionoftheseproteinsmaybethera-peuticforcancerpatients.OverexpressionofP70S6Kmaybeinvolvedinthepathogenesisoforalacin-iccellcarcinoma.
简介:【摘要】 目的 分析6s管理在物质管理中的应用价值。方法 抽选来自2017年4月至2018年4月期间我院未实施6s管理的物质管理作为对照组,将2018年5月至2019年5月期间我院采用实施6s管理的物质管理作为观察组 ,比较两组出现物品存放不合理例数、寻找物品消耗时间、清洁环境耗时、月盘点耗时。结果 观察组物品存放不合理例数显著低于对照组,差异具有统计学意义,P<0.05;观察组寻找物品消耗时间、清洁环境耗时、月盘点耗时显著短于对照组,差异具有统计学意义,P<0.05。结论 对物质管理中采用6s管理,能够避免不良事件,有助于物品核对,减少物品存放不合理例数,缩短寻找物品消耗时间、清洁环境耗时、月盘点耗时间,在临床上显示出卓越成效,值得进一步推广使用。
简介:Tosupportthescientificbasisforrapididentificationofpathogenicbacteriaandotherstud-ies,thesequencesofhsp60geneinmajor34speciesof16genusofpathogenicbacteriaweresearchoutinGenBankandaproperpairofuniversaldegenerateprimerwasdesignedbymeansofthemolecu-larbiologicalsoftwawePrimer5.0andOligo6.0.ThisprimerwasthenusedinthePCRamplification,andthehsp60genefragmentsoftheselectedpathogenicbacteriacouldbeamplifiedusingthisdegener-ateprimer.Bywayofbioinformationalanalysis,theconservation,variationandtheinterspeciesphylo-geneticrelationsofthehsp60genesequencewereanalysed.Fromtheresultsofthecomparativestudyonsequences,itwasdemonstratedthatthehsp60genewascharacterizedbyconservationandvaria-tion,inwhichtheconservedandmutantregionsco-existedandseparatelydistributedwithmanysmallmutantregionsdistributedamongtheconservedregions,justlikethemosaic.Thephylogenetictreeamongdifferentpathogenicbacteriadrawnfromthehsp60geneanalysiswasprovedtobeconsistentwiththosefrom16SrRNAand23SrRNA.Itisconcludedthatthesequencedistributionofhsp60genewouldprovideasolidbasisfortherapididentificationofpathogenicbacteriaandthedevelopmentofadiagnosticmicroarray.
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简介:摘要:目的 对 6S管理在护理技术强化训练中的应用效果予以探究。方法 选取本院 2014级护生 253名在实训教学中予以常规管理作为对照组, 2015级护生 230名在实训教学中予以 6S管理作为观察组。对两组护生护理技术实训考核成绩、教学满意度、综合素质自我评定优良情况进行对比。结果 观察组护生护理技术实训考核成绩高于对照组( P<0.05);观察组护生教学满意度 83.48%,高于对照组的 55.34%( P<0.05);除人际沟通能力与语言表达能力外,观察组护生其他项目综合素质自我评价优良例数均高于对照组( P<0.05);上述差异均有统计学意义。结论 6S管理在护理技术强化训练中的应用效果显著。
简介:Wereportedanovelmammalianreovirus,designedBYD1,isolatedfromthroatswabsofpatientswithsevereacuterespiratorysyndrome(SARS),in2003.Inthepresentstudy,wefirstlycomparedthegenomeelectrophoreticmigrationpatternsofreovirusBYD1with3prototypereovirusstrainsbypolyacrylamidegelelectrophoresis(PAGE)anddeterminedthecompletenucleotidesequenceoftheS1genesegmentofBYD1bysingleprimeramplificationtechnique.TheelectropherogramofBYD1wasdifferentfromthoseofthe3prototypestrainsandanyotherreovirusisolatesreportedbefore.TheentireS1segmentsequenceofBYD1is1437bplongwithtwomeaningfulopenreadingframes(ORFs).ThelongestORFencodesσ1,thecellattachmentprotein,andthesecondlongestORFsupposedlyencodesσ1s,animportantnonstructuralvirulencefactor.TheterminalsequencesofS1segmentare5'GCUAand3'UCAUC,whichareconsistentwiththoseofothermammalianreoviruses.Thehighesthomologyofdeducedσ1aminoacidsequenceis64%identitywithknownmammalianreoviruses.PhylogeneticanalysisofbothS1nucleotidesequenceandσ1aminoacidsequenceindicatedtheBYD1isolatebelongedtoanewcladeofserotype2group.TheresultsofthisstudyshowedthattheBYD1S1segmentwasmarkedlydifferentfromthoseofisolatesreportedbeforeandBYD1wasanovelhumanreovirusisolate.
简介:目的:基于PI3K/AKT通路探讨芪灵扶正清解颗粒对肝癌细胞凋亡的影响。方法:HepG2细胞培养条件见参考文献[2]。种板24h后,分为2组,其中空白组更换新鲜培养液培养;给药组以5mg/mL的含芪灵培养液处理,干预48h后继续后续实验。按照传统方法提取蛋白样本。结果:透射电镜结果显示与空白组相比,芪灵组细胞具备明显的早期凋亡特征,证实芪灵扶正清解颗粒能够诱导HepG2细胞凋亡。此外,本研究中芪灵组磷酸化的PI3K、AKT蛋白表达量较空白组显著下降,说明芪灵能够抑制肝癌细胞HepG2中PI3K/AKT通路的活性。结论:芪灵扶正清解颗粒促进肝癌细胞凋亡并抑制其增殖能力收可能与PI3K/AKT信号通路有关。
简介:InordertoinvestigatewhetherlipoxinA4(LXA4)hasanantagonisticeffectonlipopolysaccharide(LPS)-inducedsynthesisofinterleukin(IL)-1β,IL-6andIL-8inratpulmonarymicrovascularendothelialcells(PMVEC),andtoexplorethemolecularmechanismsofsignalpathwayinLXA4actions,culturedPMVECweretreatedwithLPS,withorwithoutpreincubationwithLXA4.ProteinsofIL-1β,IL-6andIL-8insupernatantwereanalyzedbyenzyme-linkedimmunosorbentassay(ELISA).ExpressionsofmRNAofIL-1β,IL-6andIL-8weredeterminedbyRT-PCR.Expressionsofphosphorylationofphosphoinositide3-kinase(PI3-K)andmyeloiddifferentiationfactor88(MyD88)wereanalyzedbyWesternblot.ActivitiesofDNA-bindingofnuclearfactor-kappaB(NF-κB)andactivatorprotein-1(AP-1)weremeasuredbyelectrophoreticmobilityshiftassay(EMSA).TheresultsshowedthatLPSinducedproductionofIL-1β,IL-6andIL-8inratPMVECviaMyD88/PI3-K/NF-κBandAP-1pathway-dependentsignaltransduction.LPS-stimulatedexpressionofPI3-K,activitiesofNFκBandAP-1,secretionofproteinandexpressionofmRNAofIL-1β,IL-6andIL-8butnotMyD88expressioninPMVECwereinhibitedbyLXA4inadose-dependentmanner.Inconclusion,LXA4inhibitssynthesisofIL-1β,IL-6andIL-8bydown-regulationofPI3-K/NF-κBandAP-1signalpathwayinPMVEC.
简介:ThepurposeofthisstudywastoconstructaneukaryoticDNAvectorencodingamultipleepitopeantigen(MFC)ofhepatitisCvirus(HCV)andahepatitisBsurfaceantigen(HBsAg),andexploretheeffectofHBsAggeneontheimmunityofHCVmultiple-epitopeDNAconstructinvitroandinvivoinmice.AnHCVDNAvector(pVAX1-HBs-MFC)wasconstructedbyfusingHBsAggenetotheNterminalofanHCVmultiple-epitopeantigengene.ThepVAX1-HBs-MFCwastransfectedintoHEK293TcellsanditsexpressionwasmeasuredbyELISAandWesternblotting.BALB/cmicewereintramuscularlyimmunizedwiththepVAX1-HBs-MFC,andanELISAapproachwasappliedtodeterminethespecificantibodytitersandsubtypesinthemouseserum.Thecross-reactivityoftheantibodieswasalsocheckedwithtwosynthesizedHCVhypervariableregion1(HVR1)peptides.TheIFN-γproductionandcellproliferationofthemousespleencellswereevaluatedbyELISAandMTS(3-[4,5-dimethylthiazol-2-yl]-5-[3-carboxymethoxyphenyl]-2-[4-sulfophenyl]-2H-tetrazolium,innersalt)assays,respectively.TheexpressionofpVAX1-HBs-MFCwasdetectableinthetransfectedHEK293Tcells.TheserumantibodyresponsewaseffectivelyelicitedinBALB/cmiceinjectedwithpVAX1-HBs-MFC.ThehighesttiterofantibodyagainstHCV(MFC)was1:1280,andtheratioofIgG2a/IgG1was1.50±0.12atthefifthweekafterfirstimmunization.Moreover,thecollectedmouseserumantibodyhadtheabilitytocross-reactwiththetwosynthesizedHCVHVR1peptides.ThestimulationindexofthemousesplenocytestoMFCwas1.79±0.07,andtheIFN-γlevelwas287±6pg/mlatweek21afterfirstimmunization.ThehighesttiteroftheantibodyincontrolBALB/cmiceimmunizedwithpVAX1-MFCwas1:320,andtheratioofIgG2a/IgG1was1.33±0.11atweek5post-immunization.Furthermore,thestimulationindexofthemousesplenocytescellstoMFCwas1.52+0.06,andtheIFN-γlevelwas225±9.3pg/mlatweek21post-immunization.TheHBsAggenecanenhancetheeffectsofanHCVmultiple-epitope
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