简介:Objective:Toinvestigatetheeffectofbreast-conservationtherapyinearlystagebreastcancer.Methods:Atotalof234earlystagebreastcarcinomapatientsreceivedbreastconservingtreatmentinourhospital.Aftertheoperation,theyunderwentadjuvantchemotherapyandradiotherapy.Allofthesepatientsdesiredtopreservetheirbreasts.Results:Aftermedianfollow-upof29.46months(rangefrom3to100months),3caseshadlocalrelapseand8caseshaddistantmetastasis.Theoverallsurvivalrateof5yearwas96.7%,andthediseasefreesurvivalrateof5yearwas87.85%.Conclusion:Forearlystagebreastcarcinomapatients,classicquadrantectomy,axillarydissectionandpost-operativeadjuvantchemotherapyandradiotherapyleadtoexcellentlocalcontrolandgoodsurvival.
简介:MicroRNAs(miRNAs)aresmall,non-codingsingle-strandedRNAsthatcanmodulatetargetgeneexpressionatposttranscriptionallevelandparticipateincellproliferation,differentiation,andapoptosis.Tcellshaveimportantfunctionsinacquiredimmuneresponse;miRNAsregulatethisimmuneresponsebytargetingthemRNAsofgenesinvolvedinTcelldevelopment,proliferation,differentiation,andfunction.Forinstance,miR-181familymembersfunctioninprogressionbytargetingBcl2andCD69,amongothers.MiR-17tomiR-92clustersfunctionbybindingtoCREB1,PTEN,andBim.ConsideringthatthesuppressionofTcell-mediatedimmuneresponsesagainsttumorcellsisinvolvedincancerprogression,weshouldinvestigatethemechanismbywhichmiRNAregulatesTcellstodevelopnewapproachesforcancertreatment.
简介:客观5-Aminoisoquinolinone,一水溶性,多形核白细胞(腺苷5'-diphosphate核糖)的活动的有势力禁止者聚合酶,在禁止多形核白细胞(腺苷5'-diphosphate核糖)的活动与ischaemia-reperfusion损害和发炎联系的织物损害起一个重要作用聚合酶和象ICAM-1那样的房间粘附分子的表示,P-selectin等。但是不在肿瘤它怎么样是清楚的。现在的学习的目的是在对人的脐的静脉endothelial的冒号癌线HT-29房间的粘附上学习5-Aminoisoquinolinon的效果;并且在结肠癌HT-29房间的ICAM-1,P-selectin和多形核白细胞(腺苷5'-diphosphate核糖)的活动聚合酶的表示上的5-Aminoisoquinolinon的效果。到人的脐的静脉endothelial的HT-29房间的粘附被粘合剂实验检测的方法。Immunocytochemically,Streptavidin-Peroxidase方法被用来调查ICAM-1,P-selectin和多形核白细胞(腺苷5'-diphosphate核糖)的表示(多形核白细胞(腺苷5'-diphosphate核糖)的产品聚合酶激活)。到HUVEC的HT-29房间的粘附试金的结果显示出的结果在每个5-AIQ-treated组的OD570价值是重要的,这以一种剂量依赖者方式在控制组(5-AIQ-untreated)比那降低。ICAM-1,P-selectin和多形核白细胞(腺苷5'-diphosphate核糖)的表示是重要的在HT-29房间在5-Aminoisoquinolinone-untreated组比那组织的5-Aminoisoquinolinone-treated更低。结论数据建议5-Aminoisoquinolinone能禁止HT-29房间的粘附到人的脐的静脉endothelial。5-Aminoisoquinolinone能也禁止多形核白细胞(腺苷5'-diphosphate核糖)聚合酶激活和在HT-29房间的ICAM-1和P-selectin的表情。5-Aminoisoquinolinone可能贡献肿瘤房间转移的预防。进一步的学习被需要。
简介:目的:比较592例鼻咽癌患者的CT、MRI资料,探讨MRI及CT对鼻咽癌92福州分期和02UICC/AJCC分期的不同影响。方法:分析592例治疗前进行CT/MRI检查并经病理证实为鼻咽癌的患者。比较MRI和CT检查的差异性所导致分期不同。结果:MRI分期结果较CT晚,92福州分期CT中T1+T2比例达59.4%,MRI中为39.9%(P=0.000);02UICC/AJCC分期中T1+T2比例达61.0%,MRI中为46.6%(P=0.001)。两种分期相比,92分期T4期比例为32.4%,02UICC/AJCC分期T4期比例为22.0%(P=0.001)。结论:较CT对鼻咽癌T分期的诊断具有优越性,92分期和02UICC/AJCC分期在T分期上差异不大。
简介:Inourreport,thevaluesofwholebloodserotonin(5-HT)in87apudomapatients(diagnosedbyoperationandpathology)weresummarized.Amongthem,thelevelsofurinary5-hydroxyindole-3-aceticacid(5-HIAA)of44patientswerealsotested.Theresultsshowedthatbothparametresofapudomapatientswerehigherthanthoseofnon-apudoma,post-operativepatientsofapudomaaswellasthenormal.Theincreasingextentofthelevelsofwholeblood5-HTandurinary5-HIAAinsmallintestinalcarcihoidwasthemostobviousonebutthatofrectumwasnot.Thereferablediagnosticvaluessuggestedwere:5-HT>130ngml,5-HIAA>30mg/24hours.
简介:目的检测γδT细胞信号转导分子ζ链相关蛋白-70(ζ-chainassociatedprotein70,ZAP.70)。方法分离获取健康人PBMC,用结核杆菌低分子多肽抗原(Mtb.Ag)刺激PBMC,通过流式细胞仪检测总T细胞和丫6T细胞CD69分子的动态表达;Mtb-Ag活化俩T细胞增殖培养,10d后收集细胞,用免疫磁珠阳性分选法分离获取高纯度的γδT细胞;westernblot方法检测γδT细胞内的ZAP.70分子。结果总T细胞和γδT细胞均在活化刺激24h时表达CD69分子达高峰,但总T细胞仅为16%,γδT细胞可达75.2%;新鲜分离的PBMC中γδT细胞的比例仅为4.9%,Mtb-Ag刺激培养10d后升为69.2%,免疫磁珠阳性分选后达99-3%;检测到Y6T细胞内的ZAP-70分子。结论Mtb-Ag可特异性激活俩T细胞,用Mtb-Ag刺激γδT细胞活化增殖培养,可获得大量的γδT细胞;成功地检测到细胞内ZAP-70分子,这为Y6T细胞内其它分子的检测分析奠定方法学基础,也为进一步检测γδT细胞活化信号转导过程中ZAP-70分子的激活及作用奠定基础。
简介:Forelectronicmicroscopicobservation,wefoundSSV-transformedNIH3T3cellsweredifferentfromnon-transformedcells.InSSV-transformedNIH3T3cellsnucleicytoplasmaratiowasincreasedandincytoplasmatheribosomes(polyribosomeswereattachedtotheswollenroughendoplasmicreticulum.Itwaslikelythatribosomeswerelinedtogetherfunctionallyandstructionallytoproducespecificprotein(PDGF-likeprotein).
简介:目的分析乳腺癌3.0TMRI动态增强及扩散加权成像(DWI)的表现特征。方法回顾性分析经病理检查证实的36例乳腺癌的3.0TMRI影像资料,分析其表现。结果乳腺癌MRI表现:平扫T1WI病灶呈等或稍低信号;T2WI呈等或稍高信号;肿块形状不规则,部分呈深浅不同程度分叶状,边界模糊,并见毛刺征。DWI像:所有病灶均呈高信号,ADC图呈低信号ADC值为(0.97±0.22)×10-3mm2/s。动态增强示:不均匀强化,多呈斑点状、条片状或团状,部分病灶周围血管影增多;时间-信号强度曲线:流出型(Ⅲ型)曲线29例(80.6%);平台型(Ⅱ型)型曲线5例(13.9%);流入型(Ⅰ型)曲线2例(5.5%)。36例中34例诊断为乳腺癌,2例误诊为纤维腺瘤,诊断符合率为94.4%。结论乳腺癌具有一定MRI表现特征,结合3.0TMRI动态增强及DWI检查对乳腺癌的诊断具有较高的应用价值。
简介:Objective:ToexploretheeffectsofnuclearM-CSFontheprocessoftumorigenesis.Methods:FunctionalpartofM-CSFcDNAwasinsertedintoaneukaryoticexpressionplasmidpCMV/myc/nuc,whichcanaddthreeNLStotheC-terminaloftheexpressedproteinanddirecttheproteinintothecellnuclei.TheconstructedplasmidwastransferredintoNIH3T3cellsandthecellcloneswereselectedbyG-418selection.CellclonesstableexpressingtargetproteinwereidentifiedbyRT-PCR,ABCimmunohistochemistryassayandWesternblot.Cellgrowthkineticsanalysesthroughgrowthcurves,celldoublingtime,MTTtestandanti-senseoligodeoxynucleotide(ASODN)inhibitingcellgrowthtestwereperformedtoidentifycellsproliferationpotential.Results:Thetransfectedcellsshowedelevatedproliferationpotentialoverthecontrolcells.Conclusion:AbnormalappearanceofM-CSFinnucleuscouldenhancecellproliferation,whichsuggeststhatcytokineisoformswithincellnucleusmightplaytranscriptionfactor-likerole.
简介:Objective:ToconstructamutantpEGFP-hTERTexpressionvector,toobserveitssteadyexpressionintransfectedhumanbladdercarcinomacelllineT24anditsroleinmolecularregulatorymechanismsoftelomerase,andtoprovideanewtargetgeneforbladdercancer.Methods:PCRamplificationwasperformedbyusingprimersbasedontheknowngenesequenceofhTERT.PCRproductionwasclonedintoplasmidpGEMT-TeasyandthesequenceofmutanthTERTgenewasanalyzed.ArecombinantmutanthTERTvector(pEGFP-hTERT)wasconstructedattheEcoRIandSalIsitesofthepEGFP-C1vector.AftertransfectingthefusiongeneintobladdercarcinomacelllineT24bycalciumphosphate-DNAcoprecipitation,thesteadyexpressionofGFP-hTERTfusionproteinwastestedbyfluorescentlightmicroscopy.TheproliferationchangesofbladdercarcinomacelllineT24weredetectedbylightmicroscopyandsenescencecorrelatedβ-galactosidasestaining.Results:IdentificationofpEGFP-hTERTbyenzymedigestionshowedthatmutanthTERTfragmenthadbeenclonedintoEcoRIandSalIsitesofthepEGFP-C1vector.ThesteadyexpressionofGFP-hTERTfusionproteinwaslocalizedinthenucleusoftransfectedcells.Expressionofsenescence-associatedβ-galactosidaseintransfectedcellsgraduallyincreasedwithextendedculturedtimeandcellgrowthwassuppressed.Conclusion:Themutant-typehTERTgenesuppressestheproliferationofbladdercarcinomacelllineT24bycompetitiveeffectontelomeraseactivity.ThissuggeststhathTERTgenemightbeasuitablegenetargetforbladdercancertherapy.