学科分类
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1 个结果
  • 简介:ObjectivesToestablishamethodforhighyieldmesenchymalstemcellscollection,aswellasaculturemethodforidentifyingmesenchymalstemcellsfromtheswineadipose-derivedmesenchymalstemcell(ADMSC).MethodsSwineADMSCswereisolatedfromfattissuewithcollagenase,followedbyinductionofdifferentiationtoosteogenic,adipogenicandchondrogrniccells.ThesurvivalcurveoftheADMSCatthe37oCand38oCweremeasuredusingWST-1CellProliferationAssayReagent.ResultADMSCsisolatedwithcollagenasefromswineneckfattissuegeneratedastableuniformappearanceafterthesecondgeneration.Thepassageperiodwasfivedays.ADMSCcoulddifferentiateintoosteogenic,adipogenicorchondrogrniccellsunderdifferentcultureconditions.Thehighestgrowthratewasachievedat38oCinthisstudy.ConclusionSwineADMSCshavethepotentialtodifferentiateintoosteogenic,adipogenicorchondrogrniccells,andtheymaybeappropriatefortransplantationforbothresearchandclinicalpurpose.

  • 标签: 间充质干细胞 脂肪来源 分化诱导 围网 脂肪组织 细胞分离