简介：Itisimportanttounderstandseasonalheavymetalaccumulationindifferentpartsofplantsinordertodevelopthebestphytoremediationpracticesforcontaminatedsoils.Forthispurposeweexposed,1yearoldA.victoriaeseedlingstoZnSO4in4differentconcentrations:0,50,250and500mgZnL-1for45daysovertwogrowingseasons.Subsequently,bioaccumulationofZnindifferentplanttissues（roots,shootsandleafs）wasassessedbyAtomicAbsorptionSpectroscopy（AAS）fortwoperiods.Inaddition,variousgrowthattributes（drybiomass,shootandrootlengths,plantappearance）andfunctionaltraits（leafarea,chlorophylla,bandtotal）weremeasured.TheaccumulationofZnwasinfluencedbytheZnconcentrationinthegrowthmediumandthenumberofgrowingseasons.TheamountsofZnconcentratedintheroottissuesmightindicateA.victoriaeasagoodoptionforphytostabilizationofsoilscontaminatedbyZn.WerecommendthatifA.victoriaeisusedforphytoextractionpurposes,thenitshouldbeharvestedattheendofthefirstgrowingseason（fall）becauseatthistimetheconcentrationsofZnintheabove-groundpartswillbemaximal.

简介：MYBtranscriptionfactorsrepresentafamilyofgenesthatincludetheconservedMYBDNA-bindingdomain,andtheyarewidelyinvolvedintheregulationofplantdevelopmentandsecondarymetabolism.Inthisstudy,PartofsequencesoftwoMYBtranscriptionfactorswasdeterminedthroughthecDNAmicroarrayhybridizationandselectionofcDNAlibraryderivedfromtendershoots.Thefull-lengthcDNAsofthegeneswereobtainedwithRT-PCRandRACE,andtheywere1132bpand1020bp,namedasCsMYB1andCsMYB2(GenBankaccessionNo.HQ660373andHQ660374),andcontainedORFsof879bpand675bpencoding292and224aminoacids,respectively.Sequencesanalysisshowedthatthededucedproteinmolecularweightofthetwogeneswere32.9kuand25.4ku,andtheproteinscontainedtwoconservedMYBdomainsneartheN-terminusandaconservedC1motifneartheR3domains.ThededucedaminoacidsequenceofCsMYB1andCsMYB2fromteaplantshowedhighidentitywiththatofotherplants,forinstanceCsMYB1shared57%homologywithMYB1ofGossypiumhirsutumandCsMYB2shared75%homologywithMYBC2ofVitisvinifera.Theresultofrealtime-PCRanalysisshowedthetwogeneswereexpressedconstitutivelyinalltissueswithdifferentexpressionlevels,e.g.therelativeexpressionlevelofCsMYB2inleafwashundredtimeshigherthanthatinroot.Additionally,shadingenhancedCsMYB1expression,whilethetreatmentdidnotaltertheexpressionlevelofCsMYB2.