简介:Defenceproteinsareakindofchemicaldefencecompounds.Theyplayakeyroleinplantrestrainingbioticandabioticharm.Toilluminateactivitiesofsomedefenseproteinsassociatedwithageorplantfamilyoflarch,thelarchneedleswerecollectedfromtwodifferentfamiliesofKoreanlarch,Larixolgensisandahybridlarch,L.kaempferi×L.gmelinii,respectively,andthentheactivitiesofdefenceproteinsinthoseweretestedusingaUVspectrophotometry.Theresultsshowedthattheactivitiesofprotectiveenzymesatthe16-/17-and19-year-agegroupswerehigherthanthoseattheotheragegroupsinthebothlarchspecies.Whiletheactivitiesofpolyphenoloxidase(PPO)atthe16-/17-year-agegroupandphenylalanineammonia-lyase(PAL)andproteaseinhibitorsatthe19-year-agegroupwerethehighestamongallagegroups.Then,comparedwithL.olgensis,thehybridlarchhadsignificanteffectsontheactivitiesofprotectiveenzymes.TheeffectsofplantfamilyontheactivitiesofPALandchymotrypsininhibitorweresignificantlydifferent,andthenthoseontheactivitiesofPPOwerenotsignificantlydifferent.TheeffectsofthetwofamiliesinL.olgensisontheactivitiesoftrypsininhibitor(TI)weresignificantlydifferent,whilethoseinthehybridlarchontheactivitiesofTIwerecontrary.Toconclusionsshowedthatthedifferentageandplantfamilysignificantlyaffectedtheactivitiesofdefenceproteinsintheneedlesoftwolarchspecies,andthenenhancedthelarchresistancetopests.Thesecouldplayakeyfunctioninforestrytreegeneticimprovementandmanagementinfuture.
简介:将初始体质量(0.42±0.05)g的杂交鲟(Acipenserschrenckii♀×A.baerii♂)仔鱼饲养在室内循环水养殖系统中,投喂添加0(G1)、0.25%(G2)、0.5%(G3)、0.75%(G4)和1.0%(G5)L-丙氨酰-L-谷氨酰胺(L-AG)的5种等氮饲料,每个处理3个重复,每个重复1500尾鱼,研究L-AG水平对杂交鲟体成分和十二指肠形态的影响。56d的养殖结果表明:饲料中添加0.25%和5.0%L-AG对全鱼粗蛋白含量影响不显著(P〉0.05),但添加0.75%和1.0%L-AG显著提高了全鱼粗蛋白含量(P〈0.05)。添加0.25%~1.0%L-AG对全鱼水分、粗脂肪和灰分含量影响不显著(P〉0.05)。与对照组相比,添加0.25%~1.0%L-AG对肠道绒毛高度、微绒毛高度和肌层厚度均未产生显著影响(P〉0.05)。结果表明:外源补充0.75%~1.0%L-AG可显著提高杂交鲟仔鱼粗蛋白含量,但添加0.25%~1.0%L-AG对十二指肠形态不产生显著影响。
简介:MultiplicationofElaeagnusangustifoliaL.wasexaminedinvitrosuccessivelyfromasingleshootunderthespecifiedconditionofdifferentmedia,plantgrowthregulators,pHvalueandsucroseconcentration.ItwasshowedthatMMS1wasthemostsuitablemediumonshootmultiplicationamong5mediaconcerned;BAPwasthemosteffectiveoneamongallthecytokinininvolved,BAP,KN,TDZandZT;theexpluntofthetophalf-partfromashootproducedmorenewshootsthanthatofthefoothalf-partdid;morenewshoots(>2cm)wereproducedunder3%sucrosebetweentheconcentrationswithtophalf-partexplants;shootscouldgrowwellbetweenpH4.4andpH7.0,andthebiggestnumberofshootswasproducedinpH5.6,whileinpH5.8themaximumrootingrateappeared.Asaresult,thecombinationof0.5μMBAPand0.1μMIBAonMMS1mediuminducedthemaximumshootmultiplication.Thenumberofshootamplified3timesin1month,and3^12shoots(>2cm)mightbetheoreticallymultipliedannuallyfromasingleshoot.
简介:Twopotexperimentswithacompletelyrandomdesignand4replicationswereperformedinagreenhousetoexaminetheresponsedifferenceof17cultivarsofpakchoi(BrassicachinensisL.)growninaCu-spikedandacleansoiltoCu.relativelytolerant.Whenthe17cultivarsofpakchoigrewincleangardensoil,theCuconcentrationsintheabovegroundpartwerepositivelycorrelated(r=0.6693)withtheirrootCuconcentrations.However,whentheygrewintheCu-spikedsoilahighlynegativecorrelationcoefficient(r=-0.5376)wasobtainedintheCuconcentrationbetweentheabovegroundpartandtheroot.ThismeantthattheCutolerantcultivarshadaweakabilitytotransferCufromtheirroottotheirabovegroundpart,andthereforestoredmuchmoreCuintheirrootthantheCusensitivecultivars.
简介:芒麦草是一种外来入侵植物,在我国的分布范围随时间推移呈现不断扩大的趋势。通过对文献、网络资料的整理并结合实地调查案例,汇总了芒麦草目前的分布状况。结果表明:芒麦草已经在我国10个省(包括直辖市和自治区)22个市有自然分布。芒麦草具有很强的繁殖能力、适应性和竞争能力,容易在入侵生境中建植、扩繁、归化,进而发展成为入侵植物。通过对芒麦草入侵地和原产地生境类型的比较发现,芒麦草容易在受到人为干扰或者人为活动频繁的生境中建植,结合芒麦草生物学和生态学方面的特征,对其进行风险评估。欧洲风险评估体系(WG-WRA)中,芒麦草的入侵风险评估分值为28分,入侵等级为高度入侵风险Ⅲ;澳大利亚风险评估体系(WRA)中,其入侵风险评估分值为21分,对农业和环境的分值分别为13分和10分,是一种具有高度入侵风险的植物,需要加强预警工作和引起相关部门的重视。
简介:HPLC分析表明,L.rhamnosus719在MRS中发酵时,醋酸含量先升后降,而乳酸在发酵过程中不断增加,发酵过程中,乳酸和醋酸的摩尔浓度比从6h的0.28增加到60h的2.63。L.rhamnosus719在MRS培养48h的上清液抑菌效果最好,抑菌圈直径达36mm,其中乳酸、醋酸含量分别为17.70g/L和4.32g/L。L.rhamnosus719产生较高浓度的乳酸和醋酸是其抑菌作用的主要原因。
简介:InordertomaintainhigheremergenceratewithdirectseedinginShandongrockymountain,effectsofdifferentcoveringwaysonemergencerateofSophorajaponicaL.directseedingwerestudied.Theresultsshowedthatbothmulchingfilmandgrasscovercouldeffectivelyincreasethemoisturecontentofthesoilatthedepthof0-30cm,contributingtosoilmoistureconservationandtheinhibitionofsoilevaporation,whichcreatedfavorablemoistureenvironmentforthegrowthofS.japonicaseeds.Bothth...
简介:Apotexperimentwascarriedouttostudytheeffectsofthreearbuscularmycorrhizalfungi(AMF),includingGlomusintraradices,GlomusconstrictumandGlomusmosseae,onthegrowth,rootcolonizationandCdaccumulationofmarigold(TageteserectaL.)atCdadditionlevelsof0,5and50mgkg-1insoil.Thephysiologicalcharacteristics,suchaschlorophyllcontent,solublesugarcontent,solubleproteincontentandantioxidantenzymeactivity,ofTageteserectaL.werealsoinvestigated.ThesymbioticrelationshipbetweenthemarigoldplantandarbuscularmycorrhizalfungiwaswellestablishedunderCdstress.ThesymbioticrelationshipwasreffectedbythebetterphysiobiochemicalparametersofthemarigoldplantsinoculatedwiththethreeAMFisolateswherethecolonizationratesintherootswerebetween34.3%and88.8%.Comparedwiththenon-inoculatedmarigoldplants,theshootandrootbiomassoftheinoculatedmarigoldplantsincreasedby15.2%-47.5%and47.8%-130.1%,respectively,andtheCdconcentrationandaccumulationdecreased.ThechlorophyllandsolublesugarcontentsinthemycorrhizalmarigoldplantsincreasedwithCdaddition,indicatingthatAMFinoculationhelpedthemarigoldplantstogrowbyresistingCdstress.TheantioxidantenzymesreacteddifferentlywiththethreeAMFunderCdstress.ForplantsinoculatedwithG.constrictumandG.mosseae,theactivitiesofsuperoxidedismutase(SOD)andcatalase(CAT)increasedwithincreasingCdaddition,butperoxidase(POD)activitydecreasedwithincreasingCdaddition.ForplantsinoculatedwithG.intraradices,threeoftheantioxidantenzymeactivitiesweresignificantlydecreasedathighlevelsofCdaddition.Overall,theactivitiesofthethreeantioxidantenzymesintheplantsinoculatedwithAMFwerehigherthanthoseoftheplantswithoutAMFinoculationunderCdstress.Ourresultssupporttheviewthatantioxidantenzymeshaveagreatinfluenceonthebiomassofplants,andAMFcanimprovethecapabilityofreactiveoxygenspecie
简介:Applyingtheeconomicprinciples,thisarticlecarriesouttheassessmentofeconomicperformancesbroughtbyforestryresearchachievementthroughresearchanddiscussionontheassessmentmethodofeconomicperformancesofprovenancepopularizationofPinusmassonianaL.Thearticlealsobringsintheoptionmethodtofullydisplaythevalueofforestresearchachievement.
简介:Theneedforreforestationincorkoak(QuercussuberL.)areasischallengedbydifficulties.Principalamongtheseisherbivoryofyoungplants,vegetativecompetition,andslowgrowthratesofcorkoakseedlings.WeevaluatedtheearlydevelopmentofcorkoakseedlingstreatedusingtreesheltersandmulchinginnorthwesternTunisia.Wetestedthreetreesheltertreatments(non-vented,vented,andcontrol)toshieldseedlingsfromanimaldamageandfivemulchtypestocontrolcompetingvegetation(ItalianStonePine,Lentisk,combinationofItalianStonePineandLentisk(organicmulches),gravel(inorganicmulch)andnomulch).Attheendofthetwo-yearexperiment,shelteredseedlingswere89-99%tallerthanunshelteredseedlingsandhadhighernumbersandlengthsofshootgrowthflushes.Incontrast,bothstemdiametergrowthanddryweightbiomass(fromsamplesextractedaftertwoyears)weresignificantlyreducedinsidetreeshelters.Root-to-shootratiowasnotsignificantlydifferentinshelteredvs.unshelteredseedlings,suggestingthattreesheltersdonotadverselyaffectthisparameter.Mulchingalonedidnotfavourgrowth,butcouldbebeneficialwhencombinedwithtreeshelters.Thecombinationofventedtreesheltersandgravelmulchwasthemosteffectivetreatmentforpromotingdiameter,heightandstemvolumegrowth.