简介：AnefficientandgoodDNAextractionprotocolshouldbesimple,affordableandyieldenoughDNAwithhighquality.Rice(OryzasativaL.)DNAextractionmethodsoftenuseseedlingsorleavesratherthanthegrainsandtendtobetime-consuming,involvemultiplesteps,andusehazardouschemicalsandexpensiveenzymes.RicegrainsofferseveralbenefitsoverseedlingsandleavesasasourceofDNAforgeneticanalysis.However,thesebenefitsareunderutilizedbecausethebulkofaricegrainismadeupofstarch.Itisparticularlyimportant,butdifficulttogetridofthestarchwhileextractingDNAfromricegrains.Thisco-precipitatedpolysaccharideisaknowninhibitorofDNApolymeraseactivityinpolymerasechainreaction(PCR).WedescribehereaverysimpleandhighlyaffordableChelex?-100basedDNAextractionmethodfromricegrains.Itdoesnotrequireanyhazardouschemicalsorenzymes.ThismethodreproduciblyextractsDNAwithgoodpurityindices(A260/A230andA260/A280values),butrequiresonlyafewsteps.