简介：AlmosteveryoneinChinaknowsthisstory,evenelementaryschoolpupils:Onceuponatime,therewasaweaponsalesmanclaimingwithconfidencetoonecrowdthatallhisspearswerecapableofpiercingeveryshieldthatexistedintheworld,thenheturnedaroundandtoldtheothercrowdmysteriouslythatallhisshieldswerenotpenetrable.Abystanderwhoheardbothclaimsaskedhim,"Howaboutstabbingyourshieldswithyourspears?"Itwasindeedanunexpectedquestionthatmadetheeloquentsalesmanmuterightonthespot.Sincethen,theChinesepeoplehavecalledcontradictionas"mao-dun",whichliterallymeans"thespear(pronouncedas"mao")andshield(pronouncedas"dun")".

简介：Annotationsofcompletegenomesequencessubmitteddirectlyfromsequencingprojectsarediverseintermsofannotationstrategiesandupdatefrequencies.Theseinconsistenciesmakecomparativestudiesdifficult.Toallowrapiddataprepara-tionofalargenumberofcompletegenomes,automationandspeedareimpor-tantforgenomere-annotation.Hereweintroduceanopen-sourcerapidgenomere-annotationsoftwaresystem,Restauro-G,specializedforbacterialgenomes.Restauro-Gre-annotatesagenomebysimilaritysearchesutilizingtheBLAST-LikeAlignmentTool,referringtoproteindatabasessuchasUniProtKB,NCBInr,NCBICOGs,Pfam,andPSORTb.Re-annotationbyRestauro-Gachievedover98%accuracyformostbacterialchromosomesincomparisonwiththeoriginalmanuallycuratedannotationofEMBLreleases.Restauro-GwasdevelopedinthegenericbioinformaticsworkbenchG-languageGenomeAnalysisEnvironmentandisdistributedathttp://restauro-g.iab.keio.ac.jp/undertheGNUGeneralPublicLicense.

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