简介:INTRODUCTIONPolyhydroxybutyrate(PHB)andit'scopolymerPHBVachievedbythebiosyntheticpathway,hasattractedmuchattentionasanalternationbiodegradablematerials.Theyhavetheexcellentbiocompatibilityandproperdegradationperiodsbyoptimizationofpertinentmolecularweightandcopolymercompositions.
简介:1.IntrodnctionThispaperextendsthestudyofthesingularmatrixvariatebetadistributionofrank1[1]tothecaseofageneralrank.Astherelateddistributiontonormalsampling,thematrixvariatebetadistribution(alsocalledthemultivariatebetadistribution)hasbeenstudiedextens...
简介:Aprobabilitydensityfunction(PDF)isderivedofbetadistributionwithbothλ3(skewness)andλ4(kurtosis)asparametersforweaklynonlinearwavesurfaceelevationbyuseofamethodrecentlyproposedbySrokosz.ThisPDFnotonlyhasasimplerformthanthewell-knownGram-CharlierSeriesPDFderivedbyLonguet-Higgins,butalsoovercomesanobviousshortcomingofthelatterthatwhentheseriesisunsuitablytruncated,theresultingPDFislocallynegative.TotestthederivedbetaPDF,laboratorialexperimentsofwindwavesareconducted.TheexperimentaldataindicatethatthetheoreticalrequirementsoftheparametersinthebetaPDFarefulfilled.TheexperimentalresultsshowthatthepresentPDFisinbetteragreementwiththemeasureddatathanthebetaPDFonlyincludingparameterλ3,andalsothantheGram-CharlierSeriesPDFtruncateduptothetermofH6.
简介:Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/Organophilicmontmorillonite(PHBV/OMMT)nanocompositeswerepreparedandthebiodegradabilityofthePHBV/OMMTnanocompositeswasstudiedbyacultivationdegradingmethodinsoilsuspension.TherelationshipbetweenstructureandbiodegradabilityofPHBV/OMMTnanocompositeswasinvestigated.TheresultsshowedthatthebiodegradabilityofPHBV/OMMTnanocompositesdecreasedwithincreasingamountofOMMTanditwasrelatedtothenumberofPHBVdegradingmicroorganismsindegradationenvironment,theanti-microbialpropertyofOMMTandthedegreeofcrystallinityofthenanocomposites.
简介:ObjectivesToinvestigatetheeffectsoftestosteroneenanthate(TE)onserumlipidsandlipoproteinsmetabolismandtheexpressionofandrogenreceptor(AR),estrogenreceptorbeta(ER-β)andplateletderivedgrowthfactorbeta(PDGFR-β)inaorticvascularsmoothmuscletissues(VSMTs).MethodsFortyagedmaleratswererandomlydividedinto4groups,groupA(placebogroup),groupB(2.5mg/kgintramuscularinjectionofTEonceaweek),groupC(5.0mg/kgintramuscularinjectionofTEonceaweek),groupD(10.0mg,/kgintramuscularinjectionofTEonceaweek).Allanimalswerefedfreelyduring16-weektreatmentperiods.TheexpressionofAR,ER-βandPDGFR-βwerestudiedbyWesternbolt.ResultsAverageserumLDL-CwasloweringroupDthanthatingroupA(p<0.01).Comparedwiththeothergroups,averageserumTCwasalsoloweringroupD(p<0.05).ARexpressioninaorticvascularsmoothmuscletissuescouldberegulatedbyTE:99.50±21.74,125.38±28.68and101.98±15.42forTEconcentrationsat2.5mg/kg,5.0mg/kgand10.0mg/kg,respectively,theexpressionofER-βcouldberegulatedbyTE:92.34±18.68,47.72±18.12,82.13±23.50,andtheexpressionofPDGFR-βcouldberegulatedaswellbyTE:219.70±45.59,50.16±9.72,125.36±15.74(DataforAR,ER-βandPDGFR-βproteinbandintensitywereexpressedwithx±s,withcontrolgrouptakenas100).ConclusionsThisstudyindicatesthatandrogenshavesignificanteffectsonserumlipidsandlipoproteinmetabolism.Testosteroneenanthateat5.0mg/kgcanstimulatetheexpressionofAR,butinhibitetheexpressionofPDGFR.Testosteroneenanthateattheconcentrationsof5.0mg/kgand10.0mg/kgcaninhibitetheexpressionofER-β.
简介:Texturepatternmappingisoneofthemostimportanttechniquesforhighqualityimagesyn-thesis.Itcanlargelyenhancethevisualrichnessofraster-scanimages.Inthispaperispresentedanewmethodofmappingplanartexturepatternontobeta-splinecurvedsurfaces——bilinearmappingmethodwhichcanmapplanartexturepatternontocurvedsurfaceswithlessdistortion,andalsocanfulfillthegeometrictransformationofthetexturepatternonthecurvedsurfacesbyoperatingthepatternwin-dow.ItisvaluabletobothCAD/CAMinartisticfieldandcomputergraphics.
简介:Toinvestigatetheprevalenceandgenotypeofextendedspectrumbeta-lactamases(ESBLs)mediatedbyplasmidinGram-negativebacteriafoundinsouthernChina,atotalof1184clinicalisolatesofnon-repetitivestrainsofGram-negativebacteriawerecollectedin2001from5differentcitiesinsouthernChina.TheESBLs-producingisolatesweredistinguishedbymeansofthephenotypeconfimatorytestbasedontheNCCLScriteriaandweresubjectedtoplasmidconjugationandelectroporationexperiments.Thoseclinicalisolatessucceededinplasmidtransfershadundergoneplasmidconjugationandelectro-transformation,plasmidDNAextractionandPstⅠdigestlinger-printinganalysis,aswellasthetmiversalprimerPCRamplificationoftheTEM,SHV,CTX-M,VEB,PERandSFOgenesandtheDNAsequencinginordertodeterminethegenotypesofESBLsandtheirplasmidlocations.ItwasfoundthattheincidenceoftheESBLs-producingstrainsofGram-negativebacteriawas14.6%(173/1184)with67strainsoftransconjugantsand11strainsofelectro-transformants,inwhichCTX-M-14typewas33.3%(26/78);CTX-M-3typewas23.1%(18/78);CTX-M-9typewas14.1%(11/78);CTX-M-5typewas6.4%(5/78);CTX-M-13typewas2.6%(2/78);SHV-5typewas7.7%(6/78);SHV-12typewas5.1%(4/78),SHV-2atypewas2.6%(2/78)andunidentifiedtypewas5.1%(4/78).29.5%ofthewildstrainsalsocarriedbroad-spectrumbeta-lactamasesTEM-1andSHV-1types.TheabovementionedESBLsgeneswerelocatedontransferableplasmidswithvariablesizes(from35to190kb).TheCTX-MtypeESBLswascharacterizedbyhigh-levelofresistancetocefotaxime.ItconcludedthattheCTX-M-typewasthemostprevalentgenotypeinclinicalisolatesofGram-negativebacteriainsouthernChina,andtheSHVtyperanksinthesecondplace.TEM-,VEB-,Toho-andPER-typeswerenotfoundintheseisolates.
简介:甘氨酸甜菜碱是植物细胞内一种重要的调渗物质,盐胁迫下,甘氨酸甜菜碱的积累可以保护细胞内蛋白质的结构和功能,降低细胞水势,从而增强植物自身的耐盐能力。从大肠杆菌中克隆的胆碱脱氢酶基因(betA)是甘氨酸甜菜碱合成的关键酶基因,该基因编码的胆碱脱氢酶(CDH)可将胆碱一步合成为甜菜碱。本实验室已将胆碱脱氢酶基因(betA)转入到小黑杨花粉植株基因组中,并最终获得了4个转基因株系。本研究以4个转betA基因株系(TB1、TB2、TB3、TB4)及非转基因对照为试材,在浓度为1.2%的NaCl盐胁迫下,测定其甜菜碱含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性和丙二醛(MDA)含量,并调查试材的盐害情况,计算盐害指数,目的是为了研究转基因株系的耐盐效果,从中筛选出耐盐能力较强的转基因株系。试验结果表明,4个转基因株系的甜菜碱含量均高于非转基因对照;在1.2%NaCl胁迫下TB1、TB2、TB3的超氧化物歧化酶活性、过氧化物酶活性均高于非转基因对照,TB3低于对照:TB1、TB4的丙二醛含量低于对照,TB2、TB3与对照相近。进一步的盐害分析表明4个转基因株系中的TB1和TB4株系的盐害指数低于对照的42.1%和33.4%,TB2、TB3与对照无显著差异。综合各转基因株系的耐盐性及生理指标测定结果,4个转基因株系中,TB1、TB2的耐盐性明显优于对照,有希望用于盐碱地造林及推广。
简介:CellsregulatephospholipaseD(PLD)activityinresponsetonumerousextracellularsignals.Here,weinvestigatedtheinvolvementofPLDactivityintransforminggrowthfactor-β(TGF-β1)-mediatedgrowthinhibitionofepithelialcells.TGF-β1)-mediatedgrowthinhibitionofepithelialcells.TGF-β1inhibitsthegrowthofMDCK,Mv1Lu,andA-549cells.Inthepresenceof0.4%butanol,TGF-β1inducesanincreaseintheformationofphosphatidylbutanol,auniqueproductcatalyzedbyPLD.TGF-β1alsoinducesanincreaseinphosphatidicacid(PA)levelinA-549andMDCKcells.TGF-β1inducesanincreaseinthelevelsofDAGlabeledwith[^3H]-myristicacidinA-549andMDCKcellsbutnotinMv1Lucells.NoincreaseofDAGwasobservedincellsprelabeledwith[^3H]-arachidonicacid.ThedatapresentedsuggestthatPLDactivationisinvolvedintheTGF-β1-inducedcellgrowthinhibition.
简介:Betaeffectsonsurfaceflowsinarotatingannuluswitharadialtemperaturegradientandaslopingbottomwerestudiedexperimentally.AnazimuthaljetwasproducedbytheactionoftheCoriolisforceintheconvectiveregionbetweenthetwosidewallsoftheannulus.Propagatingvelocityandpatternsofthebaroclinicwaveonthesurfacewereobtainedbyusingafrequency-meterandastreakphotographrespectively.Itisshownthatthereexiststhenonlinearinteractionbetweenthebaroclinicandbetaeffects.Thebetaeffectexertslittleinfluenceonthestratificationflowsandconstrainsthebaroclinicinstability,anditpromptstheinstabilityoftheweakstratificationflowsandresultsinthesurfacepatternofwaveswithhigherfrequency.Itisalsoindicatedthatthebetaeffectcanreducethepropagatingspeedofthesurfacewavesinthejet,andincreasethethermalRossbynumberforthosesamesurfacepatternsunderagivenTaylornumber.
简介:Blendsofpoly(3-hydroxybutyrate-co-3-hydroxyhexanoate)(PHBHHx)andpoly(butylenesuccinate-adipate)(PBSA),bothbiodegradablesemicrystallinepolyesters,werepreparedwiththeratioofPHBHHx/PBSArangingfrom80/20to20/80bymeltmixingmethod.Differentialscanningcalorimetry(DSC),thermogravimetryanalysis(TGA),dynamicmechanicalthermalanalysis(DMA),polarizingopticalmicroscopy(POM)andwideangleX-raydiffractometer(WAXD)wereusedtostudythemiscibilityandcrystallizationbehaviorofPHBHHx/PBSAblends.ExperimentalresultsindicatethatPHBHHxisimmisciblewithPBSAasshownbythealmostunchangedglasstransitiontemperatureandthebiphasicmelt.