简介：Thematrixmetalloproteinases(MMPs)areafamilyofzine-dependentendopeptidasesthatplayakeyroleinbothnormalandpathologicalprocessesinvolvingtissueremodelingevents.Theexpressionoftheseproteolyticenzymesishighlyregulatedbyabalancebetweenextracellularmatrix(ECM)depositionanditsdegradation,andiscontrolledbygrowthfactors,cytokines,hormones,aswellasinteractionswiththeECMmacromolecules.Furthermore,theactivityoftheMMPsisregulatedbytheirnaturalendogenousinhibitors,whicharemembersofthetissueinhibitorofmetalloproteinases(TIMP)family.Inthenormalmammarygland,MMPsareexpressedduringductaldevelopment,lobulo-alveolardevelopmentinpregnancyandinvolutionafterlactation.Underpathologicalconditions,suchastumorigenesis,thedysregulatedexpressionofMMPsplayaroleintumorinitiation,progressionandmalignantconversionaswellasfacilitatinginvasionandmetastasisofmalignantcellsthroughdegradationoftheECMandbasementmembranes.

简介：Objective:ToconstructtherecombinantplasmidcontainingGlycerophosphodiesterphosphodiesterase(Gpd)genefromTreponemapallidumandtransfectitintoHelacellstoexpresstheencodedoutermembraneprotein.Methods:TheGpdgenewasamplifiedfromthegenomicDNAofT.pallidumbypolymerasechainreaction(PCR)andinsertedintocloningvectorpUCm-T.TheinsertedGpdgenewassubclonedintotheappropriatesiteofpcDNA3.1(+)vector.Afteridentificationbysequencingandrestrictiveenzymesdigestion,therecombinantplasmidwastransfectedintoHelacellsusingliposomes.TheexpressedproteinwasidentifiedbyimmunocytochemistryandWesternblot.Results:ThetargetGpdgenesegmentwasapproximately1059bp.TheDNAsequenceoftheGpdgenecontainedinthepcDNA3.1(+)vectorwasconsistentwiththepublishednucleotidesequence.ThehomologyofthenucleotideandputativeaminoacidsequencesoftheGpdgenebetweenT.pallidumsubsp.pallidumNicholsandvariouspathogenictreponemalstrainsrangedfrom98%to100%.ImmunocytochemistryandWesternblotanalysisshowedthattheconstructedGpd-pcDNA3.1(+)vectorexpressedafusionproteinwithacalculatedmolecularmassof41KDainHelacellsandthattheexpressedproteinreactedwiththeserafromsyphilispatients.Conclusion:ThesuccessfulconstructionandexpressionoftheeukaryoticexpressionplasmidoftheGpdgenefromT.pallidumprovideapromisingtooltofurtherstudythebiologicalactivityofT.pallidumanddevelopaDNAvaccineforsyphilis.

简介：Duringtheevolvementofarchitecture,theresearchonarchitecturalformandstructureaswellasdifferentwaystoexpressandrealizearchitecturalthoughtsisconstantlythefocusofmanyarchitects,Thispaperillustratesthemutualinfluenceofthewaysofexpressionanddesignofarchitecturetopeople'sunderstandingofthemeaningofarchitecturethroughoutthehistoryoftheireffortsandexploration.thepaperalsodiscussestheextensiveusageofvirtual-realithapproachthroughcomputertechnology,Itpointsoutthatthetraditionalarchitecturalstandpointwillundergobreakthroughbytheemergenceofnewexpressionanddesignmethod.Inthisway,people'sunderstandingofarchitecturewillbemoreprofoundandlasting.

简介：Objective:Toclone,sequenceandexpresstheprimateβ-chemokineRANTESgenes,hRANTESfromH.sapiensandmRANTESfromM.Mulatta,inordertoexplorethepossibilityofAIDSgenetherapy.Methods:hRANTESandmRANTESwereamplifiedbyreversetranscription-polymerasechainreaction(RT-PCR)fromRNAsextractedfromphytoagglutinin(PHA)-activatedperipheralbloodlymphocytes,hRANTESwascloned,sequencedandexpressedinvitro,andmRANTESwasdirectlysequencedforhomologycomparison.Results:Anexpected276bpfragmentwasobtainedinbothamplifications,andsequencedatademonstratedarelativelyhighhomologyamongdifferentcopiesofhRANTES(97%),andhRANTESwasupto95.6%homologoustomRANTES.WhencomparedwithRANTESfromothermammals,hRANTESgaverisetoahomologyrangingfrom77%to86%.TheclonedhRANTESwasexpressedinvitroandapositivesignalofRANTESwasdetectedbydotblotting.Conclusion:Thefull-lengthofhRANTESsequencewassubmittedtoGenBankandhadbeenreleased.OurmRANTESsequenceisfirstreportedandnotyetappearedinGenBank.ThesuccessfulcloningandexpressionofhRANTESwillprovideabasisforAIDSgenetherapyinthefuture.

简介：

简介：obtainaninitialoverviewofgenediversityandexpressionpatterninporcinethymus,11,712ESTs(ExpressedSequenceTags)from100-day-oldporcinethymus(FTY)weresequencedand7,071cleanedESTswereusedforgeneexpressionanalysis.ClusteredbythePHRAPprogram,959contigsand3,074singletswereobtained.Blastsearchshowedthat806contigsand1,669singlets(totally5,442ESTs)hadhomologuesinGenBankand1,629ESTswerenovel.AccordingtotheGeneOntologyclassification,36.99%ESTswerecatalogedintothegeneexpressiongroup,indicatingthatalthoughthefunctionalgene(18.78%indefensegroup)ofthymusisexpressedinacertaindegree,the100-day-oldporcinethymusstillexistsinadevelopmentalstage.Comparativeanalysisshowedthatthegeneexpressionpatternofthe100-day-oldporcinethymusissimilartothatofthehumaninfantthymus.

简介：

简介：

简介：ObjectivesToconstructarecombinantplasmidcarryingenhancedgreenfluorescentprotein(EGFP)andhumanvascularendothelialgrowthfactor(VEGF)121geneanddetectitsexpressioninratmesenchymalstemcells(MSCs).MethodsHumanVEGF121cDNAwasamplifiedwithpolymerasechainreaction(PCR)frompCD/hVEGF121andwasinsertedintotheeukaryoticexpressionvectorpEGFPC1.AfterbeingidentifiedwithPCR,doubleenzymedigestionandDNAsequencing.TherecombinantplasmidpEGFP/hVEGF121wastransferredintoratMSCswithlipofectamine.TheexpressionofEGFP/VEGF121fusionproteinweredetectedwithfluorescencemicroscopeandimmunocytochemicalstainingrespectively.ResultsTherecombinantplasmidwasconfirmedwithPCR,doubleenzymedigestionandDNAsequencing.ThefluorescencemicroscopeandimmunocytochemicalstainingresultsshowedthattheEGFPandVEGF121proteinwereexpressedinMSCs48haftertransfection.ConclusionsTherecombinantplasmidcarryingEGFPandhumanVEGFwassuccessfullyconstructedandexpressedpositivelyinratMSCs.ItoffersapromisetoolforfurtherresearchondifferentiationofMSCsandVEGFgenetherapyforischemialcardiovasculardisease.

简介：Ananalysissolutionofrateequationisderivedforverticalcavitysurface-emittinglasers.BasedontheenhancedspontaneousemissioncausedbyVCSELsandinfluenceofnonradiativerecombination,therelationbetweenoutputpropertiesandstructuralparametersofmulti-quantumwells(MQWs)isobtanined.Itwasfoundthatthecharacteristiccurveofa"thresholdless"laserisstronglynonradiativedepopulation-dependent.Whenthenonradiativedepopulationisnozero,thelight-currentcharacteristicisnotlinearlyevenforanidealclosedmicrocavity.Thelightoutputisincreasedbytheenhancedwellnumberandbythereducedwidth.Inparticular,alowerthresholdcurrentdensityforMQWsstructureintheshortcavityisrealizedbyus,meanwhilethesharpnessofthevariationdependsonspontaneousemissionfactor.

简介：WithDNA-mRNAhybridizationinsitutechnique,theexpressionoffiveoncogenes,c-N-ras,c-Ki-ras.c-Ha-ras,c-mycandc-fos,wasobservedintwocasesofhumanhepatocellularcarcinoma.Theexpressionofc-N-ras&c-foswasgreatlyenhancedintumortissuesofthetwocases,andabout25%-50%ofthetumorcellsshowedpositiveexpression.Theotherthreeoncogenesnamelyc-Ki-ras,c-Ha-ras&c-myc,werenotdetectedinthesetwocarcinomasorinthenon-cancerouslivertissuesadjacenttothecarcinomas.Itissurmisedthatc-N-rasandc-fosmayplaycoordinativeroleinmaintainingthemalignantphenotypeofhumanprimaryhepatocellularcarcinoma.

简介：Acriticaldifferencebetweentherighthemispherehypothesisandvalencehypothesisofemotionprocessingiswhethertheprocessingofhappyfacialexpressionsislateralizedtotherightorlefthemisphere.InthisstudyparticipantsfromaChinesesamplewereaskedtoclassifyhappyorneutralfacialexpressionspresentedeitherbilaterallyinbothvisualfieldsorunilaterallyintheleftvisualfield(LVF)orrightvisualfield(RVF).Theywererequiredtomakethespeededresponsesusingeithertheleftorrighthand.Itwasfoundthatforbothleftandrighthandresponses,happy(andneutral)expressionspresentedintheLVFwereidentifiedfasterthanhappy(andneutral)expressionspresentedintheRVF.BilateralpresentationshowednofurtheradvantageoverLVFpresentation.Moreover,lefthandresponsesweregenerallyfasterthanrighthandresponses,althoughthiseffectwasmorepronouncedforneutralexpression.Thesefindingswereinterpretedassupportingtherighthemispherehypothesis,withhappyexpressionbeingidentifiedinitiallybytherighthemisphere.

简介：Clusterinisa75-80kDaheterodimericglycoprotein,thatisproducedinmosttissuesbutwhichexactbiologicalroleisstillnotclear.Particularly,itsroleinprotectionorpromotionofapoptosisisheavilydisputed,sincedatasupportingbothviewshavebeenreportedinseveralindependentstudies.Toclarifythisissue,andalsotodeterminewhetherclusterinexpressionitselfmightbeaffectedbyapoptosis,inthepresentstudy,ratthymocytesweretreatedwithdexamethasone,-asyntheticglucocorticoidthatelicitsapoptosisinthymocytes-,andclusterinmRNAexpressionwasanalyzedbysemi-quantitativeRT-PCRbeforeandafterinductionofapoptosis.Interestingly,neitherthetreatmentwithdexamethasoneinvitronortriggeringofapoptosisinvivoup-regulatedclusterinexpression,opposingtheviewthatclusterinisinvolvedinapoptoticprocesses.Ontheotherhand,anewclusterinmRNAisoformwasdetectedandisolated,whoseexpressionwasrestrictedtofreshlyisolatedthymocytes.Thisnovelisoformlacksthepost-translationalproteolyticcleavagesiteandisthereforepredictedtoencodeamonomericprotein.Thebiologicalfunctionundernormalcircumstances,however,willneedfurtherinvestigationsforclarification.Whileapoptosiscouldnotmodulateclusterinexpression,activationofthymocyteswithconcanavalinAandinterleukin-2resultedinup-regulationofclusterinmRNAlevel,indicatingthatclusterinexpressionisratherunderthecontrolofcellactivation-mediatedratherthanapoptosis-inducedsignals.

简介：Carotenepigmentsinflowersandfruitsaredistinctfeaturesrelatedtofitnessadvantagessuchasattractinginsectsforpollinationandbirdsforseeddispersal.Inpapaya,thefleshcolorofthefruitisconsideredaqualitytraitthatcorrelateswithnutritionalvalueandislinkedtoshelf-lifeofthefruit.Toelucidatethecarotenoidbiosynthesispathwayinpapaya,wetookacandidategeneapproachtoclonethelycopeneβ-cyclasegene,LCY-B.ApapayaLCY-Bortholog,cpLCY-B,wassuccessfullyidentifiedfrombothcDNAandbacterialartificialchromosome(BAC)librariesandcompletegenomicsequencewasobtainedfromthepositiveBACincludingthepromoterregion.ThiscpLCY-Bshared80%aminoacididentitywithcitrusLCY-B.However,fullgenomicsequencesfrombothyellow-andred-fleshedpapayawereidentical.Quantitativereal-timePCR(qPCR)revealedsimilarlevelsofexpressionatsixdifferentmaturingstagesoffruitsforbothyellow-andred-fleshedgenotypes.FurtherexpressionanalysesofcpLCY-Bshowedthatitsexpressionlevelswereseven-andthree-foldhigherinleavesand,respectively,flowersthaninfruits,suggestingthatcpLCY-Bisdown-regulatedduringthefruitripeningprocess.

简介：Arithmeticcodingisarelativelynewloss-lessdatacompressiontechniquethathasattractedmuchattentioninrecentyears.Weshowtheiterationofbit-levelarithmeticcodingcanbespecifiedbyacontinuousfunction.Theanalysisexpressionandsomepropertiesofthisfunctionarediscussed.Anapplicationofthefunctionisprovidedforexploringthesecurityofarithmeticcodeswhentheyareusedfordataencryption.

简介：客观：在在人的创伤的奔流和正常透镜的上皮的房间之间的原子factor-KB(NF-κB)的表示学习差别。方法：全部的RNAof前面的囊标本从受不了创伤的奔流做半量的RT-PCR并且在在他们之间的NF-κB的表示进行差别的分析的正常cadaveric眼睛施主和那些在显微镜下面被拿。结果：作为与在正常控制组的0.8337的平均数相比，NF-κB的表示等价物为在创伤的奔流患者的透镜的上皮的房间是0.9074，并且差别具有显著意义(t=2.447，P<0.05)因此。结论：NF-κB是可能的对必要的一种抄写因素维持正常透镜的上皮的房间的新陈代谢。在创伤的奔流患者可得到的更高的NF-κB“透镜的上皮的房间工具NF-κB具有到创伤的奔流的出现和开发的可能的关联的s。

简介：Objective:TostudytheimmunologicalmechanismsofCondylomaacuminata(CA)throughinvestigatingTlymphocytesubsetlevelsandcytokineprofileintheperipheralbloodofpatientswithCondylomaAcuminata.Methods:Tricolorandbicolorimmunofluorescentstainingantibodyofcellsurfaceantigenandintracel-lularIL-2,IL-4,IL-12,IFN-γinCD4^+andCD8^+T-lymphocytesfrom20patientswithCAwereperformedandfollowedbyflowcytometry.Results:ThenumberofCD3^+T,CD4^+T-lymphocytescellsandCD4^+/CD8^+Tcellsratioweresignificantlydecreased(P<0.01)inpatientswithCAComparedtocontrols,andIL-2,IL-12,IFN-γproductioninCD4^+Tcellswasdecreased(P<0.01),IL-4andIFN-γproduc-tioninCD4^+Tcellswasnotsignificantlydifferent(P>0.05),whileIL-2andIL-12productioninCD8^+Tccellswasdecreased(P<0.01),whereasIFN-γandIL-4pro-ducinginCD4^+Tcellswereofnosignificantlydifference(P>0.05).Conclusions:TherewasanimbalanceofTlympho-cytesubsets,Th1/Th2cytokinesandTc1/Tc2intheperipheralbloodofCApatients,whichmayplayanimportantroleinthepathogenesisandprogressionofCA.

简介：在大多数谷物庄稼，phytic酸是磷的主要存储形式，它能减少磷酸盐的简历可获得性。phytase的转基因的表示被认为是在转基因的植物免除磷酸盐phytate的一个有效方法。在这研究，工厂表示向量，包含重组体phytase基因由玉米ubiquitin(Ubi)开车倡导者经由Agrobacterium-mediatedtransformation被构造并且介绍进一个精英米饭变化。在实验期间，15根独立转基因的米饭线的一个总数被改革。PCR和南部的污点的结果显示目标基因集成于转基因的米饭工厂的染色体。而且，提取fromtheimmature几根转基因的线播种的全部的RNA的RT-PCR分析证明重组体phytase基因能通常被表示。无机的磷内容，两个都比在untransformed野生型在转基因的工厂在成熟种子和叶是显著地更高的。

简介：Ourobjectiveistosolvethelactosemalabsorptionandintoleranceofhumanbeingsbycombiningmlcro-ecologypathwithgeneticengineeringtechnique.PlasmidpMG36ewasusedtocloneandexpressaβ-galactosidasegenefromL.delbrueckiibulgaricusstrain1.1480intheLactococcuslactissubsp,cremorisMG1363andLactococcuslactissubsp.lactisIL1403.TherecombinantplasmidwaspreservedandproliferatedinEscherichiacoli(E.coli)JM109,andtransformedintoMG1363and1L1403byelectroporation.Theproteinexpressionwasstudied.(1)Thebifidobacteriumculturemedium(BBL)wassuitableforthegrowthofthestrain1.1480.(2)With13aminoacidsattheN-terminusfromthevector,β-galactosidasefusionprotein(whichretainedtheenzymeactivity)couldbesuccessfullyexpressedinE.coliJM109,MG1363andIL1403,buttheexpressionquantitywaslargerintheformerthaninthelattertwo.(3)TheSDsequencedesignedcouldbesuccessfullyrecognizedbyboththeE.coliandtheLactococcuslactis,buttheexpressionlevelofthenon-fusionβ-galac-tosidaseproteinwaslowerthanthatofthefusionproteininthesamehost.Theβ-galactosidasegeneticallyengineeredE.coliJM109isausefultooltoproducethisenzymeinvitro.Thesignalpeptideoftheusp45proteinfromtheLactococcuslactiscanbeaddedbeforethepromotersequencetopromoteβ-galactosidasesecretionfromLactococcuslactis.Thepotentialapplicationoftheβ-galactosidasegeneticallyengineeredMG1363andIL1403tocurethelactosemalabsorptionandlactoseintoleranceinbothhealthfoodandmedicineispromising。

简介：Thenaturalmeasureofacertainareainphasespaceisdefinedfirstly.Onthebasisofnaturalmeasure,theexpressionofLyapunovexponentbasedonunstableperiodicorbits(UPOs)ofchaoticsystemsisdeducedfromtheoreticalaspect.Then,bymeansoftheinherentrelationbetweenUPOsandsystematicLyapunovexponent,thetransitionalmechanismandrouteofchaoticsystemsfromlow-dimensionalchaostohigh-dimensionalchaosareexplained.Intheend,anovelmethodforcomputingsystematicLyapunovexponentsbasedonUPOsisproposed.Itscomputingprocedureisalsosummarized.ThechaoticsystemdescribedbyHenonmapistakenasexample.ThroughcalculatingtheLypunovexponentsofthissystem,validityofthesuggestedmethodisverified.