简介：摘要目的探讨Fat1对食管鳞癌(ESCC)细胞增殖能力的影响及其机制。方法以Plko.1-puro-GFP-shRNA-Fat1质粒转染KYSE450细胞，应用实时定量PCR检测Fat1敲低效率。采用四甲基偶氮唑蓝法检测Fat1和细胞外调节蛋白激酶(ERK)通路抑制剂U0126对KYSE450细胞增殖能力的影响，平板克隆实验观察Fat1对ESCC细胞克隆形成能力的影响，活细胞动态观察细胞周期，Western blot方法检测相关靶蛋白的表达。通过裸鼠体内成瘤实验检测KYSE450细胞敲低Fat1后对移植瘤生长的影响，免疫组化方法检测肿瘤组织中相关蛋白的表达。结果Fat1sh1组和Fat1sh2组细胞中Fat1敲低效率分别为(77.1±6.9)%和(77.7±7.1)%。与对照组相比，Fat1sh1组和Fat1sh2组细胞增殖明显加快(P<0.05)，细胞中p-ERK1/2的表达明显增加。经U0126处理后，Fat1敲低促进KYSE450细胞增殖的作用消失，细胞中p-ERK1/2的表达下降到与对照组相近的水平。对照组的细胞克隆数为(72±8)个，Fat1sh1组和Fat1sh2组分别为(155±28)个和(193±9)个，均高于对照组(均P<0.05)。对照组细胞分裂1次的时间为(1 622±32)min，Fat1sh1组细胞分裂1次的时间为(1 408±29)min，比对照组明显缩短(P<0.05)。裸鼠成瘤实验显示，Fat1敲低组比对照组裸鼠移植瘤增长速度快，接种后4周，对照组和Fat1敲低组瘤体重量分别为(0.224±0.028)g和(1.532±0.196)g，差异有统计学意义(P<0.05)。结论Fat1可通过抑制ERK信号通路进而抑制ESCC细胞增殖。

简介：摘要目的探讨Fat1对食管鳞癌(ESCC)细胞增殖能力的影响及其机制。方法以Plko.1-puro-GFP-shRNA-Fat1质粒转染KYSE450细胞，应用实时定量PCR检测Fat1敲低效率。采用四甲基偶氮唑蓝法检测Fat1和细胞外调节蛋白激酶(ERK)通路抑制剂U0126对KYSE450细胞增殖能力的影响，平板克隆实验观察Fat1对ESCC细胞克隆形成能力的影响，活细胞动态观察细胞周期，Western blot方法检测相关靶蛋白的表达。通过裸鼠体内成瘤实验检测KYSE450细胞敲低Fat1后对移植瘤生长的影响，免疫组化方法检测肿瘤组织中相关蛋白的表达。结果Fat1sh1组和Fat1sh2组细胞中Fat1敲低效率分别为(77.1±6.9)%和(77.7±7.1)%。与对照组相比，Fat1sh1组和Fat1sh2组细胞增殖明显加快(P<0.05)，细胞中p-ERK1/2的表达明显增加。经U0126处理后，Fat1敲低促进KYSE450细胞增殖的作用消失，细胞中p-ERK1/2的表达下降到与对照组相近的水平。对照组的细胞克隆数为(72±8)个，Fat1sh1组和Fat1sh2组分别为(155±28)个和(193±9)个，均高于对照组(均P<0.05)。对照组细胞分裂1次的时间为(1 622±32)min，Fat1sh1组细胞分裂1次的时间为(1 408±29)min，比对照组明显缩短(P<0.05)。裸鼠成瘤实验显示，Fat1敲低组比对照组裸鼠移植瘤增长速度快，接种后4周，对照组和Fat1敲低组瘤体重量分别为(0.224±0.028)g和(1.532±0.196)g，差异有统计学意义(P<0.05)。结论Fat1可通过抑制ERK信号通路进而抑制ESCC细胞增殖。

简介：AlexiswritingastoryforRob＇sshow.Alocalwomanfoundagiantratinherhouse.Itwassittingonamatinherlivingroom,anditchasedherblackcataway.Thestorysoundslikeakid,spoembecausemanyofthewordshavesimilarsounds.＂Afatratchasesawoman＇sblackcat!＂

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简介：在这篇文章，胖solenoidal引起注意的人的一个班的随机的不安被考虑。我们显示出他们的不变的密度的坚韧性和由调查他们的转移操作员的性质在随机的不安下面混合的率。关键词胖solenoidal引起注意的人-随机的不安-Sinai-Ruelle-Bowen措施先生(2000)题目分类37D25-37D30

简介：Thenecessitytodistributethedataoverthewideareanetwork(WAN)tothephysicists'homeinstituteswillincrease,andtheeffectiveutilizationofthenetworkbecomescrucial,However,networksinthefutureWANwilltypicallyhavealargebandwidthatanorderofgigabitpersecond,withalatencyofseveralhundredssecondssothatthelargebandwidth-delayprodeuctextendstotensofmegabytesandnumerousproblemsareencountered.suchnetworksarecalled"longfatnetworks(LFNs)"Inordertostudythedatatransferoperatingonalongfatnetwork,wehavebuiltthePCclustersconnectedwiththerouterwhichcansimulatebandwidthlimitations.delays,packetlosses,andmultipatheffects.thisrouterisrunningonFreeBSDwithDUMMYNETkerneloption.Onthesemachineswehavemeasuredtheperformanceofthebulkdatatransfterwithnumerousconditionsandstudiedtheeffecienttransftermethods.

简介：Objective:Toimprovethediagnosisandtreatmentofseverecerebralfatembolism(SCFE).Methods:ThedataofninepatientswithSCFEwereretrospectivelyanalyzed.Themanifestationsofthecentralnervesystem,respiratorysystemandhemorrhagewererecorded,atthesametime,accessoryexaminationincludingarterialoxygen,fatmacroglobulesinvenousbloodandimageexaminationwasadapted.Thepatientsweretreatedwithexopexy,pharmocotherapyandoxygentherapy.Results:Twooftheninepatientsdiedofseverecomplications,theothersevenrecoveredwithoutseveresequela.Conclusions:GurdstandardshouldbeimprovedforearlydiagnosisofSCFE.Ifsverecomplicationscanbeprevented,patientswhoreceiveearlytreatmentwillhavefavourableprognosis.

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简介：随着WindowsXP的日益普及，NTFS文件系统也渐渐被大家熟悉．不过在FAT32／16／DOS的分区中无法直接对NTFS文件系统的访问，这也给大家的使用带来一定的困难．如WindowsXP无法登录又需要修改该分区的文仕时，以前我们一般通过“NTFSFORDOS”这个软件来实现在DOS下对NTFS又件系统的读取．不过“NTFSFORDOS”一般只支持对NTFS的“只读”操作。

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简介：AbstractFat embolism syndrome (FES) is a serious life-threatening manifestation of the fat embolism phenomenon characterized by Bergman’s triad of dyspnea, petechiae and mental confusion. While fat embolization into systemic circulation is common, FES occurs in a meagre 0.05%-3% of patients having isolated long bone fractures. Though visual symptoms are commonly attributed to fat embolism retinopathy and is a later occurrence, it may not always be the case. Cortical blindness has been seldom reported in association with FES, and less so as a presenting complaint. Furthermore, no previous literature has described the same in context of an isolated tibia fracture. We report a 20-year-old gentleman with an isolated right tibia shaft fracture who developed sudden onset diminution of vision in both eyes less than 24 h following trauma with no other complaints. Lack of any remarkable ophthalmoscopic findings or other symptoms left us with a diagnostic conundrum. He later went on to develop altered mentation, hypoxia and generalized tonic-clonic seizures with subsequent MRI revealing multiple cerebral fat emboli also involving both occipital lobes. Supportive measures were instituted and his general condition as well as vision gradually improved following which he underwent plate fixation of the fracture under spinal anaesthesia. The perioperative period was uneventful and he was discharged following staple removal. At one month of follow-up, the patient had no residual visual field defects or neurological deficits. Though FES is rare among isolated tibia fractures, this clinical catastrophe may strike in any unsuspected setting thereby warranting a high index of suspicion to ensure early diagnosis and improved patient outcomes.

简介：Purpose:Theaimofthisstudywastoanalyzeandcomparetheeffectsofdifferentsports(swimming,football,basketball,andhandball)onfatmassandleanmassinprepubertalandpubertalgirls.Methods:Twohundredgirls(10.6±1.5yearsold,TannerstagesI–III)participatedinthestudyandweredividedinto5groups:40swimmers,40footballplayers,40basketballplayers,40handballplayers,and40controls.Fatandleanmassesatwholebody,arms,trunk,andlegsweremeasuredusingdual-energyX-rayabsorptiometry(DXA).PubertalstatuswasdeterminedusingTannertest.Effectsofdifferentsportsonfatandleanmasseswereassessedthroughanalysisofcovariancewithheightascovariates.Analyseswereperformedseparatelyin2groupsdependingontheTannerstage(prepubertalandpubertal).Results:Thegirlsofthecontrolgrouphadlessleanmassandmorefatmasscomparedtothegirlswhoplaysports(p<0.05).Thereweredifferencesinbodyfatbetweensports.Theswimmersandfootballplayershadlessbodyfat(p<0.05).Ontheotherhand,handballplayersshowedthehighestvaluesinleanmass(p<0.05).Conclusion:Impactsports(football,basketball,andhandball)andlow-impactsports(swimming)provideanappropriatedevelopmentofleanmassingrowinggirls.Wecanconcludethatpeoplepracticingsportsatearlyagesensurealowerfatmassandhigherleanmasscomparedtothosewhodonotpractice.Theseresultsmaybeusefulasapreventivemethodofadultobesity.

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