简介:摘要:脉冲功率技术在油井解堵、管道清洗等领域有良好的应用前景,储能电容器的远距离充电是其中的关键技术之一。本文研究了基于LC谐振变换器的远距离恒流充电技术,首先应用交流小信号分析法对基于LC谐振变换器的恒流充电电源基本工作原理进行了说明。最后通过对50 μF电容器的充电实验,证明了基于LC谐振变换器的充电电路具有具有恒流、结构简单、控制方便等特性,适用于脉冲功率装置储能电容的长距离快速、高效充电。
简介:AbstractBackground:The human brain is the most complex organ in the body, and it is important to have a better understanding of how the protein composition in the brain regions contributes to the pathogenesis of associated neurological disorders.Methods:In this study, a comparative analysis of the frontal and temporal cortex proteomes was conducted by isobaric tags of relative and absolute quantification (iTRAQ) labeling and two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS). Brain protein was taken from relatively normal tissue that could not be avoided of damage during emergent surgery of the TBI (traumatic brain injury) patients admitted in Beijing Tiantan Hospital from 2014 to 2017. Eight cases were included. Four frontal lobes and 4 temporal lobes proteome were analyzed and the proteins were quantitated. Gene Ontology (GO), Ingenuity Pathway Analysis (IPA), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were used to analyze the biological function of identified proteins, unchanged proteins, and differentially expressed proteins (DEPs).Results:A total number of 2127 protein groups were identified in the frontal and temporal lobe proteomes. A total of 1709 proteins could be quantitated in both the frontal and temporal cortex. Among 90 DEPs, 14 proteins were screened highly expressed in the temporal cortex, including MAPT, SNCG, ATP5IF1, GAP43, HSPE1, STMN1, NDUFS6, LDHB, SNCB, NDUFA7, MRPS36, EPDR1, CISD1, and RALA. In addition, compared to proteins expressed in the frontal cortex, 14 proteins including EDC4, NIT2, VWF, ASTN1, TGM2, SSB, CLU, HBA1, STOM, CRP, LRG1, SAA2, S100A4, and VTN were a low expression in the temporal cortex. The biological process enrichment showed that unchanged proteins between the frontal and temporal cortex mainly take part in regulated exocytosis, axon guidance, and vesicle-mediated transport. The KEGG pathway analysis showed that unchanged proteins between the frontal and temporal cortex mainly take part in oxidative phosphorylation, carbon metabolism, Huntington's disease, and Parkinson's disease.Conclusions:The majority of proteins are unchanged between the frontal and temporal cortex, and unchanged proteins are closely related to its function. Among DEPs, MATP (tau) is upregulated in the temporal cortex, closely related to Alzheimer's disease (AD), and is one of the targets for the treatment of AD. CLU is downregulated in the temporal cortex which functions as an extracellular chaperone that prevents aggregation of non-native proteins. It was suggested that the temporal lobe may not be the "functional dumb area" of the traditional view, but could be involved in important neural metabolic circuits.
简介:摘要:急性胆囊炎伴胆囊结石患者在疾病早期常出现呕吐、突发性腹痛、胆绞痛等临床症状,随着病情进展还可引发感染性休克、胆囊坏疽、穿孔以及多器官功能损伤,对患者生命安全威胁极大,需及早通过手术治疗。随着腹腔镜技术的不断发展,腹腔镜胆囊切除术(LC)已成为临床治疗胆结石、胆囊炎等疾病的主要术式。虽然LC具有创伤小、恢复快、并发症少等优势,但作为外科手术仍存在一定风险,而且LC手术部位较为特殊,术前穿刺和手术过程中均可能引发胆管损伤,不利于患者术后恢复。本研究探讨急性胆囊炎伴胆囊结石患者腹腔镜胆囊切除术(LC)术后胆管损伤的相关影响因素,以减少胆管损伤、改善患者预后,现报道如下。
简介:摘要:本文建立了一种利用液相色谱-串联质谱法LC-Ms/Ms测定蜂产品中氯霉素残留量的方法。样本提取、净化后,经乙腈-水流动相体系洗脱,C18色谱柱分析后,利用三重四级杆液相色谱质谱联用仪灵敏度高、专属性强、定量准确来检测氯霉素,可以用来快速筛查蜂蜜和蜂王浆中的氯霉素残留。
简介:摘要目的探讨腹腔镜胆囊切除术(LC)联合腹腔镜胆总管探查术(LCBDE)治疗对老年胆囊结石合并胆总管结石患者疼痛应激和炎症因子影响。方法回顾性分析复旦大学附属华东医院2020年1月—2021年1月老年胆囊结石合并胆总管结石患者80例,按照手术方法分为观察组与对照组,每组40例。观察组患者采用LC联合LCBDE治疗,对照组患者采用传统开放手术治疗。比较两组患者围术期指标和并发症情况,术前和术后3 d疼痛应激和炎症因子变化,及术前、术后1个月和术后6个月生活质量情况。正态分布的计量资料采用均数±标准差(Mean±SD)表示,组间比较采用t检验。计数资料组间比较采用χ2检验。结果观察组手术时间、下床活动时间、术后排气时间、术中出血量、术后并发症发生率分别为(98.39±7.23) min、(1.56±0.37) d、(1.29±0.28) d、(38.94±5.64) mL、5%,对照组分别为(107.53±9.98) min、(2.53±0.52) d、(2.16±0.34) d、(65.87±7.96) mL、25%,观察组各指标均优于对照组,两组相比差异具有统计学意义(P<0.05)。两组患者术后3 d血清5-羟色胺、P物质和去甲肾上腺素水平均高于术前(P<0.05);观察组患者术后3 d血清5-羟色胺、P物质和去甲肾上腺素分别为(0.70±0.12) pg/mL、(175.42±17.87) ng/mL和(378.52±26.57) ng/mL,对照组分别为(1.02±0.18) pg/mL、(248.98±18.98) ng/mL和(460.92±35.42) ng/mL,观察组各指标均小于对照组,两组相比差异具有统计学意义(P<0.05)。两组患者术后3 d患者血清肿瘤坏死因子-α、C反应蛋白和白介素-6水平高于术前(P<0.05);观察组患者术后3 d血清肿瘤坏死因子-α、C反应蛋白和白介素-6分别为(108.76±10.86) pg/mL、(14.23±3.18) mg/L和(17.84±3.98) pg/mL,对照组分别为(156.95±16.67) pg/mL、(26.52±4.59) mg/L和(28.53±5.67) pg/mL,观察组各指标均小于对照组,两组相比差异具有统计学意义(P<0.05)。两组术后1个月和术后6个月生活质量评分高于术前(P<0.05);观察组患者术后1个月和术后6个月生活质量评分均高于对照组(P<0.05)。结论LC联合LCBDE治疗对老年胆囊结石合并胆总管结石患者疼痛应激和炎症因子影响小,且术后并发症少,可改善患者生活质量。
简介:摘要目的探讨急性胆囊炎行经皮经肝胆囊穿刺引流术(PTGBD)后序贯行腹腔镜胆囊切除术(LC)的最佳手术时机。方法回顾性分析2016年4月至2020年10月郑州大学附属郑州中心医院收治的先行PTGBD再行LC的218例急性胆囊炎患者的临床资料。根据患者PTGBD后行LC间隔的时间分为3组:PTGBD后3~4周行LC者为短间隔组(n=67);PTGBD后5~8周行LC者为中间隔组(n=78);PTGBD后>8周行LC者为长间隔组(n=73)。对比3组患者的基线资料及围手术期情况。结果218例患者中,男性97例,女性121例,年龄(72.1±8.4)岁。LC术前短间隔组患者胆囊壁厚度(4.77±0.62)mm大于中间隔组(3.85±0.34)mm和长间隔组(3.81±0.25)mm,差异具有统计学意义(P<0.05)。中间隔组患者的术中出血量低于短间隔组,差异具有统计学意义(P<0.05)。中间隔组在手术时间、中转开腹、放置引流管、术后住院时间、二次住院费用方面,均优于其余两组,差异具有统计学意义(均P<0.05)。中间隔组患者并发症发生率低于短间隔组[2.56%(2/78)比14.93%(10/67)]和长间隔组[2.56%(2/78)比12.33%(9/73)],差异具有统计学意义(P<0.05)。结论急性胆囊炎PTGBD后序贯行LC的最佳手术时机为PTGBD后5~8周。
简介:摘要目的探讨自噬相关基因Beclin-1、LC3和p62在食管鳞状细胞癌(ESCC)中的表达及意义。方法回顾性分析2015年1月至2016年12月解放军陆军第八十一集团军医院接受手术治疗的112例原发性ESCC患者的临床资料。免疫组织化学法检测112例ESCC组织及31例癌旁正常食管黏膜组织中Beclin-1、p62和LC3蛋白的表达情况,分析3种自噬相关标志物在ESCC中的表达及其与患者临床病理特征的关系。结果112例ESCC组织中Beclin-1、LC3和p62阳性表达率分别为32.14%(36/112)、37.50%(42/112)和63.39%(71/112),31例癌旁正常食管黏膜阳性表达率分别为61.29%(19/31)、64.52%(20/31)和32.26%(10/31),差异均有统计学意义(χ2值分别为8.715、7.216、9.584,均P<0.01)。Beclin-1、LC3在ESCC中的阳性表达率均低于癌旁正常食管黏膜,p62在ESCC中的阳性表达率则高于癌旁正常食管黏膜。Beclin-1表达与ESCC患者组织学分级、肿瘤浸润深度、TNM分期、是否存在淋巴结转移均有关(均P<0.05);LC3表达与ESCC患者肿瘤浸润深度、TNM分期均有关(均P<0.01);p62表达与ESCC患者是否存在淋巴结转移有关(P<0.01)。在ESCC中,LC3与Beclin-1的表达呈正相关(r=0.731,P=0.001),而与p62的表达呈负相关(r=-0.215,P=0.023)。结论自噬在ESCC的发生、发展中发挥一定作用,联合检测自噬相关基因Beclin-1、p62和LC3可辅助临床诊断并指导后续综合治疗。