简介：Objective:MemorystemTcells(Tscm)haveattractedattentionbecauseoftheirenhancedself-renewal,multipotentcapacity,andanti-tumorcapacities.However,littleisknownaboutTscminpatientswithrenalclearcellcarcinoma(RCC)andtheroleofWntsignalinginthesecells.WeevaluatedTscmfromRCCpatientsconcerningtheiractivationofWntsignalinginvitroandexploredthemechanismofpreferentialsurvival.Methods:FlowcytometryidentifiedsurfacemarkersandcytokinesproducedfromaccumulatedTscminthepresenceoftheglycogensynthasekinasebetainhibitorTWS119.Apoptosiswasevaluatedafterinductionusingtumornecrosisfactor-alpha.ImmunofluorescenceandWesternblotanalyseswereusedtoinvestigatetheactivationofthenuclearfactor-kappaB(NF-КB)pathway.Results:RCCpatientshadasimilarpercentageofCD4~+andCD8~+Tscmashealthydonors.ActivationofWntsignalingbyTWS119resultedintheaccumulationofTscminactivatedTcells,butreversalofdifferentiatedTcellstoTscmwasnotachieved.PreferentialsurvivalofTscmwasassociatedwithincreasedanti-apoptoticabilitymediateddownstreamoftheNF-КBactivationpathway.Conclusions:ThefindingthatTscmcanaccumulatebyWntsignalinginvitroinbloodfromRCCpatientswillhelpindevisingnewcancertherapystrategiesofTscm-basedadoptiveimmunotherapy,suchasdendriticcell-stimulatedTscm,andTcellreceptororchimericantigenreceptor-engineeredTscm.

简介：Theinsectmidgutepitheliumiscomposedofcolumnar,goblet,andregenerativecells.Columnarepithelialcellsarethemostabundantandhavemembraneprotrusionsthatformthebrushbordermembrane(BBM)ontheirapicalside.Theseincreasesurfaceareaavailableforthetransportofnutrients,butalsoprovideopportunitiesforinteractionwithxenobioticssuchaspathogens,toxinsandhostplantallelochemicals.RecentimprovementsinproteomicandbioinfbrmaticstoolsprovidedanopportunitytodeterminetheproteomeoftheT.niBBMinunprecedenteddetail.ThisstudyreportstheidentificationofproteinsfromBBMvesicles(BBMVs)usingsingledimensionpolyacrylamidegelelec?trophoresiscoupledwithmulti-dimensionalproteinidentificationtechnology.Morethan3000proteinswereassociatedwiththeBBMVofwhich697werepredictedtopossesseitherasignalpeptide,atleastonetransmembranedomainoraGPI-anchorsignal.Ofthese,bioinfbrmaticsanalysisandmanualcurationpredictedthat185maybeassociatedwiththeBBMVorepithelialcellplasmamembrane.Thesearediscussedwithrespecttotheirpredictedfunctions,namelydigestion,nutrientuptake,cellsignaling,development,cell-cellinteractions,andotherfunctions.Webelievethistobethemostdetailedproteomicanalysisofthelepidopteranmidgutepitheliummembranetodate,whichwillprovideinformationtobetterunderstandthebiochemical,physiologicalandpathologicalprocessestakingplaceinthelarvalmidgut.

简介：可是《色戒》这个小说里头写的是一个杀汉奸的故事,张爱玲的小说《色戒》之所以被李安看中,他的电影《色戒》这几天在香港、台湾首映

简介：我们在人生中可能经常会迷失,经常失去方向。歌手JasonMraz演唱的这首IWon'tGiveup(《我不会放弃》),将他永不放弃的精神传递给了每一个人。

简介：Objective:ToexaminetheeffectofpSer9-GSK-3βonbreastcancerandtodeterminewhethertheunderlyingmetabolicandimmunologicalmechanismisassociatedwithROS/eIF2Bandnaturalkiller(NK)cells.Methods:WeemployedTWS119toinactivateGSK-3βbyphosphorylatingSer9andexploreditseffectonbreastcancerandNKcells.TheexpressionofGSK-3β,naturalkillergroup2memberD(NKG2D)ligands,eIF2BwasquantifiedbyPCRandWesternblot.Wemeasuredintracellularreactiveoxygenspecies(ROS)andmitochondrialROSusingDCFH-DAandMitoSOXTMprobe,respectively,andconductedquantitativeanalysisofcellularrespirationon4T1cellswithmitochondrialrespiratorychaincomplexⅠ/Ⅲkits.Results:OurinvestigationrevealedthatTWS119downregulatedNKG2Dligands(H60aandRae1),suppressedthecytotoxicityofNKcells,andpromotedthemigrationof4T1murinebreastcancercells.Nevertheless,LY290042,whichattenuatesp-GSK-3βformationbyinhibitingthePI3K/Aktpathway,reversedtheseeffects.WealsofoundthathigherexpressionofpSer9-GSK-3βinducedhigherlevelsofROS,andobservedthatabnormalityofmitochondrialrespiratorychaincomplexⅠ/ⅢfunctioninducedthedysfunctionofGSK-3β-inducedelectrontransportchain,naturallydisturbingtheROSlevel.Inaddition,theexpressionofNOX3andNOX4wassignificantlyup-regulated,whichaffectedthegenerationofROSandassociatedwiththemetastasisofbreastcancer.Furthermore,wefoundthattheexpressionofpSer535-eIF2BpromotedtheexpressionofNKG2Dligands(Mult-1andRae1)followingbyexpressionofpSer9-GSK-3βandgenerationofROS.Conclusions:ThePI3K/Akt/GSK-3β/ROS/eIF2BpathwaycouldregulateNKcellactivityandsensitivityoftumorcellstoNKcells,whichresultedinbreastcancergrowthandlungmetastasis.Thus,GSK-3βisapromisingtargetofanti-tumortherapy.

简介：目的采用Meta分析评价3.0TMRT2mapping及T1ρ区分膝骨关节炎(KOA)患者与健康人的价值。方法检索中国知网、维普、万方数据库及PubMed、EMBASE、CochraneLibrary数据库中采用3.0TMRT2mapping和/或T1ρ区分KOA患者(KOA组)与健康人(对照组)的临床对照研究,检索时间截至2018年7月31日。采用Stata14.0软件分析KOA组与对照组、重度KOA与轻度KOA患者权重均数差值(WMD)及其95%CI。结果纳入文献15篇,KOA组420例、对照组450名。KOA组总体[WMD=4.06,95%CI(2.73,5.39)]、轻度KOA[WMD=2.35,95%CI(1.15,3.55)]、重度KOA[WMD=8.07,95%CI(4.03,12.12)]及未分度KOA患者[WMD=1.58,95%CI(0.51,2.66)]关节软骨T2值均高于对照组(P均<0.01),重度KOA高于轻度KOA[WMD=4.66,95%CI(1.86,7.46),P<0.01]。KOA组总体[WMD=5.41,95%CI(3.59,7.22)]、轻度KOA[WMD=4.25,95%CI(2.32,6.18)]、重度KOA[WMD=8.44,95%CI(6.24,10.64)]及未分度KOA患者[WMD=3.51,95%CI(1.23,5.79)]关节软骨T1ρ值均高于对照组(P均<0.01),且重度KOA高于轻度KOA患者[WMD=6.33,95%CI(2.02,10.64),P<0.01]。结论利用3.0TMRT2mapping及T1ρ成像可区分KOA患者与健康人,鉴别轻度与重度KOA。

简介：摘要随着社会经济水平的不断提高，人们也对工程质量也提出了更好的要求。在桥梁技术施工过程中日渐改进，而桥梁工程中T梁施工是一个重要的施工环节，其混凝土强度直接影响公路桥梁运行的安全性、稳定性及耐久性。因此，应重视公路桥梁T梁混凝土强度施工质量控制，而混凝土强度上升的快慢由养护温度起着决定性的作用尤其是在冬季。通过宜毕高速公路预制T梁冬季智能养护系统的实际应用，T梁智能养护系统能够解决混凝土强度随着温度变化增长的问题，能够有效的控制混凝土质量，消除混凝土强度不满足设计要求的质量隐患，而且节能环保无污染、加快施工进度、减少投入，并且取得了较好的经济效益和社会效益，得到业主及质监站一致好评。

简介：通用会议管理（GCC）模块的实现基于T.124协议,GCC使用MCS提供的域管理功能来管理会议,实现数据会议服务器的基本通信功能

简介：摘要目的探讨甲状腺疾病患者的T3、T4及TSH水平变化情况以及临床意义。方法随机抽取2017年01月-2018年12月本院收治的58例甲状腺疾病病患，其中30例甲亢患者作A组，28例甲减患者作B组，再选择同期入院进行体检的30例健康人作C组，均对其T3、T4及TSH水平展开测定，并观察检测结果。结果A组T3及T4水平明显比B组及C组高，而TSH水平则比B组及C组低，（P＜0.05）。B组T3水平明显比A组及C组低，T4水平比A组低，比C组高，而TSH水平则比A组及C组高，（P＜0.05）。A组及B组T3、T4、TSH水平与C组对比均有显著性，（P＜0.05）。结论T3、T4及TSH水平变化情况不仅可直接反映甲状腺疾病患者病情，还能将其视作辨别甲减与甲亢的重要指标，从而实现对患者甲状腺功能的准确评价目标。

简介：在模型设计上,模型设计时,模型设计采用如下荷载组合对强度进行验算

简介：摘要由于各种原因造成省煤器管失效，发生局部破坏，甚至导致停炉事故。严重影响了锅炉的使用安全性和效率。本文提出对省煤器管旧系改造安装方案。

简介：

简介：WehavesynthesizedandinvestigatedphysicalpropertiesoftwonewquaternarycompoundsGd2CoAl4T2(T=Si,Ge)singlecrystals,whichareisostructuraltoTb2NiAl4Ge2andEr2CoAl4Ge2.Themostimportantstructuralfeatureofthesematerialsistheanti-CaF2-typeCoAl4T2slabs.Thesematerialsshowmetallicbehaviorbelow300Kandthereisalong-rangeantiferromagnetic(AFM)transitionappearingat20and27KforGdCoAl4Ge2andGd2CoAl4Si2,respectively.ResistivityandheatcapacitymeasurementsalsoconfirmthesebulkAFMtransitions.Furtheranalysisindicatesthatthislong-rangeantiferromagnetismshouldresultfromthemagneticinteractionbetweenlocalmomentsofGd^3+ions.

简介：Tomeettherequirementsofelectronicvehicles(EVs)andhybridelectricvehicles(HEVs),thehighenergydensityLiNi0.8Co0.15Al0.05O2(NCA)cathodeandSi–Canodehaveattractedmoreattention.HerewereportthethermalbehaviorsofNCA/Si–Cpouchcellduringthecharge/dischargeprocessesatdifferentcurrentdensities.ThetotalheatgenerationsarederivedfromthesurfacetemperaturechangeduringelectrochemicalLi+insertion/extractioninadiabaticsurrounding.Thereversibleheatisdeterminedbytheentropiccoefficients,whicharerelatedwithopen-circuitvoltageatdifferenttemperatures;whiletheirreversibleheatisdeterminedbytheinternalresistance,whichcanbeobtainedviaV–Icharacteristic,electrochemicalimpedancespectroscopyandhybridpulsepowercharacterization(HPPC).Duringtheelectrochemicalprocess,thereversibleheatcontributeslessthan10%tototalheatgeneration;andtheheatgeneratedinchargeprocessislessthanthatindischargeprocess.Theresultsofthermalbehaviorsanalysesareconducivetounderstandingthesafetymanagementandpavingthewayforbuildingareliablethermalmodelofhighenergydensitylithiumionbattery.

简介：摘要近几年，我国正在积极研究预制混凝土双T板吊装施工方法，尽管在一定程度上已取得一定的成效，但是仍在谋陷方面有些许不足之处。通过现场的观察及查阅相关预制混凝土双T板吊装施工方法等方面的专著，本文对双T板吊装施工方法出现的问题进行分析，并提出以下几方面的技术措施。

简介：摘要近几年，我国正在积极研究预制混凝土双T板吊装施工方法，尽管在一定程度上已取得一定的成效，但是仍在谋陷方面有些许不足之处。通过现场的观察及查阅相关预制混凝土双T板吊装施工方法等方面的专著，本文对双T板吊装施工方法出现的问题进行分析，并提出以下几方面的技术措施。

简介：摘要本文以蒙西至华中地区铁路煤运通道工程中交第三航务工程局有限公司君山制梁场简支T梁架设为例，分析重载铁路双线T梁架设施工组织安排的合理性，探讨DJ-180公铁两用架桥机架设工艺。

简介：23loopmloop8movdx,(4)检测端口1F7H的B7、B3位,moval

简介：摘要镗床是一种精密加工机床,主要用于加工精确的孔和孔间距离要求较为精确的零件。卧式镗床具有万能特点。本文可采用可编程控制器(PLC)对卧式镗床控制系统进行改造，提高工作效率。

简介：摘要桥梁连续体施工技术，其关键技术之一是转动体进行称配重。本文重点对墩顶转体称配重进行分析，确保桥梁顺利转体提供依据，对同类型工程具有借鉴意义。