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简介：直译：像马一样吃东西。

简介：Tobesickasadog.直译：病得像狗一样。意译：病得很严重。

简介：直译：裤子里有蚂蚁。意译：形容焦躁不安的心情。

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简介：Genestellcellswhattodo,forexample,whentorepairDNAmistakesorwhentodie,andcanbeturnedonorofflikealightswitch.Knowingwhichgenesareswitchedon,orexpressed,isimportantforthetreatmentandmonitoringofdisease.Now,forthefirsttime,Researchersinthelaboratoryof

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简介：Thematrixmetalloproteinases(MMPs)areafamilyofzine-dependentendopeptidasesthatplayakeyroleinbothnormalandpathologicalprocessesinvolvingtissueremodelingevents.Theexpressionoftheseproteolyticenzymesishighlyregulatedbyabalancebetweenextracellularmatrix(ECM)depositionanditsdegradation,andiscontrolledbygrowthfactors,cytokines,hormones,aswellasinteractionswiththeECMmacromolecules.Furthermore,theactivityoftheMMPsisregulatedbytheirnaturalendogenousinhibitors,whicharemembersofthetissueinhibitorofmetalloproteinases(TIMP)family.Inthenormalmammarygland,MMPsareexpressedduringductaldevelopment,lobulo-alveolardevelopmentinpregnancyandinvolutionafterlactation.Underpathologicalconditions,suchastumorigenesis,thedysregulatedexpressionofMMPsplayaroleintumorinitiation,progressionandmalignantconversionaswellasfacilitatinginvasionandmetastasisofmalignantcellsthroughdegradationoftheECMandbasementmembranes.

简介：AIM:ToscreenmicroRNAs（miRNAs）andsetuptargetmiRNAsinpterygium.METHODS:PrimaryfibroblastswereisolatedfrompterygiumandTenon’scapsuleandcultured.ImmunocytochemicalanalysisandWesternblottingwereperformedtoconfirmthecultureoffibroblasts.Inall,1733miRNAswerescreenedinthefirststepbyusingGeneChip?miRNA3.0Array.SpecificmiRNAsinvolvedinthepathogenesisofpterygiumweresubsequentlydeterminedusingthefollowingcriteria:1)highreproducibilityinarepetitivetest;2)baselogvalueof>7.0forbothcontrolandpterygialfibroblasts;and3)logratioof>1.0betweenpterygialfibroblastsandcontrolfibroblasts.RESULTS:Primaryscreeningshowedthat887/1733miRNAswereup-regulatedand846/1733miRNAsweredown-regulatedinpterygialfibroblastscomparedwiththoseincontrolfibroblasts.Ofthe1733miRNAsscreened,4miRNAs,namely,miRNA-143a-3p,miRNA-181a-2-3p,miRNA-377-5pandmiRNA-411a-5p,mettheabove-mentionedcriteria.Primaryscreeningshowedthatthese4miRNAswereup-regulatedinpterygialfibroblastscomparedwithcontrolfibroblastsandthatmiRNA-143a-3phadthehighestmeanratiocomparedwiththemiRNAsincontrolfibroblasts.CONCLUSION:miRNA-143a-3p,miRNA-181a-2-3p,miRNA-377-5pandmiRNA-411a-5pareup-regulatedinpterygialfibroblastscomparedwithcontrolfibroblasts,suggestingtheirinvolvementinthepathogenesisofpterygium.

简介：Objective:ToconstructtherecombinantplasmidcontainingGlycerophosphodiesterphosphodiesterase(Gpd)genefromTreponemapallidumandtransfectitintoHelacellstoexpresstheencodedoutermembraneprotein.Methods:TheGpdgenewasamplifiedfromthegenomicDNAofT.pallidumbypolymerasechainreaction(PCR)andinsertedintocloningvectorpUCm-T.TheinsertedGpdgenewassubclonedintotheappropriatesiteofpcDNA3.1(+)vector.Afteridentificationbysequencingandrestrictiveenzymesdigestion,therecombinantplasmidwastransfectedintoHelacellsusingliposomes.TheexpressedproteinwasidentifiedbyimmunocytochemistryandWesternblot.Results:ThetargetGpdgenesegmentwasapproximately1059bp.TheDNAsequenceoftheGpdgenecontainedinthepcDNA3.1(+)vectorwasconsistentwiththepublishednucleotidesequence.ThehomologyofthenucleotideandputativeaminoacidsequencesoftheGpdgenebetweenT.pallidumsubsp.pallidumNicholsandvariouspathogenictreponemalstrainsrangedfrom98%to100%.ImmunocytochemistryandWesternblotanalysisshowedthattheconstructedGpd-pcDNA3.1(+)vectorexpressedafusionproteinwithacalculatedmolecularmassof41KDainHelacellsandthattheexpressedproteinreactedwiththeserafromsyphilispatients.Conclusion:ThesuccessfulconstructionandexpressionoftheeukaryoticexpressionplasmidoftheGpdgenefromT.pallidumprovideapromisingtooltofurtherstudythebiologicalactivityofT.pallidumanddevelopaDNAvaccineforsyphilis.

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简介：Duringtheevolvementofarchitecture,theresearchonarchitecturalformandstructureaswellasdifferentwaystoexpressandrealizearchitecturalthoughtsisconstantlythefocusofmanyarchitects,Thispaperillustratesthemutualinfluenceofthewaysofexpressionanddesignofarchitecturetopeople'sunderstandingofthemeaningofarchitecturethroughoutthehistoryoftheireffortsandexploration.thepaperalsodiscussestheextensiveusageofvirtual-realithapproachthroughcomputertechnology,Itpointsoutthatthetraditionalarchitecturalstandpointwillundergobreakthroughbytheemergenceofnewexpressionanddesignmethod.Inthisway,people'sunderstandingofarchitecturewillbemoreprofoundandlasting.

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简介：Objective:Toclone,sequenceandexpresstheprimateβ-chemokineRANTESgenes,hRANTESfromH.sapiensandmRANTESfromM.Mulatta,inordertoexplorethepossibilityofAIDSgenetherapy.Methods:hRANTESandmRANTESwereamplifiedbyreversetranscription-polymerasechainreaction(RT-PCR)fromRNAsextractedfromphytoagglutinin(PHA)-activatedperipheralbloodlymphocytes,hRANTESwascloned,sequencedandexpressedinvitro,andmRANTESwasdirectlysequencedforhomologycomparison.Results:Anexpected276bpfragmentwasobtainedinbothamplifications,andsequencedatademonstratedarelativelyhighhomologyamongdifferentcopiesofhRANTES(97%),andhRANTESwasupto95.6%homologoustomRANTES.WhencomparedwithRANTESfromothermammals,hRANTESgaverisetoahomologyrangingfrom77%to86%.TheclonedhRANTESwasexpressedinvitroandapositivesignalofRANTESwasdetectedbydotblotting.Conclusion:Thefull-lengthofhRANTESsequencewassubmittedtoGenBankandhadbeenreleased.OurmRANTESsequenceisfirstreportedandnotyetappearedinGenBank.ThesuccessfulcloningandexpressionofhRANTESwillprovideabasisforAIDSgenetherapyinthefuture.

简介：多字的表情(MWE)在自然语言经常并且不合语法地出现。在免费文章识别MWE是一个很挑战性的问题。这份报纸建议没有知识、无指导、语言无关的多字的表示距离(MED)。新度量标准从一个接受物理原则被导出，从n克测量距离到它的语义，并且超过在二应用的MWE上的另外的最先进的方法：问答和命名实体抽取。

简介：Objective:ToconstructsurvivinshRNAexpressionvectorcartingenhancedgreenfluorescentproteingene,transfectitintoGBC-SDHcellsviaelectroporation,andgetGBC-SDcellswhicharestableexpressingsurvivinshRNA.Methods:ThesiRNAsequencetargetingsurvivinmRNAwassynthesizedandclonedintopEGFP-H1.TheconstructedplasmidandpEGFP-H1weretransfectedintoGBC-SDcellsrespectivelyvialiposome,andthetransfectingeffectwasdetectedwithFlowCytometry.ThenthetransfectedcellswereselectedwithG418.Results:Therecombinantplasmidwassuccessfullyconstructed,namedpEGFP-survivin.ThegenetransfectionefficienciesinpEGFP-H1-transfectedgroupandpEGFP-survivin-transfectedgroupwerethe80.29%±2.71%and83.85%±2.34%(P>0.05),whichwassuccessfultogetthecellsthatarestableexpressingshRNA,namedGBC-SD/EGFPandGBC-SD/survivin.Conclusion:SurvivinshRNAexpressionvectorwasconstructedsuccessfullyandgotGBC-SDcellswhicharestableexpressionshRNA.

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简介：FADD是在死亡的一个重要proapoptotic适配器导致受体的apoptosis。最近，FADD被发现了参予许多non-apoptotic过程，例如开发，房间周期前进和幸存。它的non-apoptotic活动被在C终端区域定位的丝氨酸残余的phosphorylated地位调整，从proapoptotic函数不同的域联系了DED和DD域。由于在自然FADD的表示和结晶化的困难，然而，迄今为止，所有FADD变体的分子的结构没包含C终端区域。阐明C终端区域的结构功能关系，我们需要获得FADD变体那个包含的C终端区域。在这研究，包含DD领域的鼠标FADD(80-205)和C终端区域，指定了为C-FADD，在E被表示。有在N终点的他的标签的coli并且由Ni2+亲密关系层析净化了。净化的蛋白质在glutaraldehydecross-linking分析作为同质的单体存在并且在CD(圆形的二色性)展出了一个典型螺旋系列试金。在vitro他的标签，下拉试金证明净化的C-FADD拥有了CK为它的non-apoptotic功能重要的我有约束力的活动。

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