简介：Tofindouttheoptimalconcentration,infusionrateanddosageofsalineforresuscitation.Methods:Forty-fivedogswereusedtoestablishhypovolemicshockmodels.Thedogswereresuscitatedwithsalineofdifferentconcentrationsanddifferentdosagesunderdifferentinfusionrates,andtheresuscitationresultswerecompared.Results:Thebestconcentrationwas7.5%,thebestrateofinfusion20ml/min(avolumeequivalentto15%oftheshedblood)andthebestdosage5.71ml/kg.Themethodwaseffectiveforresuscitation,themeanarterialpressure(MAP)couldbeelevatedto89%ofthebaseline,andthisMAPcouldbekeptformorethanonehour.Conclusions:Using7.5%sodiumchloridesolutionequivalentto15%oftheshedbloodataninfusionrateof20ml/mincanachieveabestresuscitationresult.

简介：Objective:Tostudytheeffectofmethylprednisolone(MP)onreperfusioninjuryinsevereuncontrolledhemorrhagicshockandexplorethepossiblemechanisminvolved.Methods:Twelvedogswererandomlydividedintotwogroups,controlgroup(GroupI,n=6)andMPgroup(GroupII,n=6).Theanimalswerebledcontinuouslyfromafemoralarterycathetertoproduceuncontrolledhemorrhagicshockmodels.ResuscitationwithlactatedRinger's(LR)solutionwasinitiatedwhenmeanarterialpressure(MAP)decreasedto20mmHg,andMAPwasmaintainedat30-40mmHg.MP(4mg/kg)wasinjectedintravenouslyinGroupIIwhenresuscitationbegan.WhileinGroupI,normalsaline(NS)wasinjectedinstead.Thelevelsofsuperoxidedismutase(SOD)andmalondialdehyde(MDA)weremeasuredbeforeexsanguination(T1),whenMAPdecreasedto20mmHg(T2),60min(T3)and120min(T4)afterresuscitation.Heartrate,MAPandcardiacoutput(CO)levelswererecordedconcomitantly.Results:InfusionvolumeandhemorrhagevolumeshedfromthesuperiormesentericarteryinGroupIwerehigherthanthoseinGroupII(P<0.01andP<0.05).Afterreperfusion,bloodSODlevelsdecreasedprogressivelyandMDAlevelsincreasedrapidlyinGroupI.InGroupII,bloodSODlevelsatT3andT4decreasedascomparedwiththatatT1butastepwiseincreasewaspresent.AtT4,bloodSODlevelwassignificantlyhigherinGroupIIthaninGroupI(P<0.01).AtT3andT4,MDAlevelsweremarkedlylowerinGroupIIthaninGroupI.Duringreperfusion,MAPwasmoresteadyinGroupIIthaninGroupIandsurvivalrateafter120min(atT4)washigherinGroupIIthaninGroupI(P<0.05).Conclusions:MPhasaprotectiveeffectonsevereuncontrolledhemorrhagicshockandsubsequentreperfusioninjury.Themechanismmainlyinvolvestheanti-lipidperoxidationactivityofMP.

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简介：Inordertoanalyzethenucleoprotein(NP)geneofCrimean-Congohemorrhagicfevervirus(CCHFV),viralRNAwasamplifiedbyRT-PCRbyusingtheproof-readingDNApolymerasetoproducethecompleteNPgene.ThePCRproductwassequenced,analyzedforphylogenesisandclonedintotheexpressionvectorpE132aandtherecombinantplasmidexpressedinE.coilBL-21withhighyield.Theprimarilypurifiedfusedprotein.wasusedtocoatELISAplatesforthedetectantibodies.ItwasfoundthesimilaritiesbetweenNPgeneofBA88166andotherXHFVsinnucleotidelevelandaminoacidcontentswereverysignificant,andtheNPgeneofBA88166encodedanucleoproteinwith482aminoacidandadeducedmolecularweight(MW)of54kDa.Westernblotassayshowedthatthefusionproteinexpressedinbacteriapossessedgoodantigenicity.TheresultswithELISAforthedetectionofthehumanandanimalseracollectedinendemicareaswerefoundtobeingoodaccordancetotheclinicaldiagnosis.ItconcludedthattherelationsofNPgenesofXHFVBA88166andotherXHFVsappearedtobeevolutionallyclose.Themethodologiesestablishedinthisstudywereaccurate,specific,rapidandreproduciblefortheclinicalexaminationsandepidemiologicalsurvey.

简介：客观：在试验性的出血性的吃惊老鼠在心肌层和hepatocyte线粒体的功能上调查海水沉浸的效果。方法:24只男Wistar老鼠被划分成三个组(8在各个组织的n=)：控制组，HSL组(土地上的出血性的吃惊组)和HSS组(在海水的出血性的吃惊组)。血液动力学的参数，H~+-ATPase(adenosinetriphosphatase)的活动，succinatedehydrogenase(同步数字系列)和Ca~(2+)-Mg~(2+)-ATPase，在心肌层和hepatocytemitochondria的钙内容被测量，越过内部mitochondrialmembrane的质子translocation的变化被分析。结果：hernodynamic索引和H~+-ATPase的活动，同步数字系列，在HSS组的Ca~(2+)-Mg~(2+)-ATPase是比在控制组和HSLgroup的那些显著地低的(P<0.05)。在HSS组，在心肌层andhepatocyte的织物和线粒体的钙层次与控制组和HSL组相比显著地被提高(P<0.05)。在在三个组之中的质子translocation的Therewas不重要的差别。结论：Thisinvestigation证明那海水沉浸能加重出血性的吃惊老鼠的条件。

简介：Objective:Toinvestigatethetherapeuticeffectofcisaprideongastricinjuryfollowinghemorrhagicshockresuscitation.Methods:108Wistarratsweighing(200g±30g)wererandomlydividedintoashamshock(SS)group(n=36),ahemorrhagicshockresuscitation(HS)group(n=36)andahemorrhagicshockcisapridetreated(HSC)group(n=36).Samplingat1,2and4hoursafterresuscitationwasdoneand6samplesforeachobservationitemweretaken.Thegastricbloodflowvolumewasmeasuredbyisotopelabelbiologicalmicroglobulin.GastricpHi,gastricemptying,MDAandNa+-K+-ATPaseofgastricmucosaweremeasured.Results:IntheHSCgroup,therelativeresidualrateofgastricpigmentdecreasedsignificantly,thegastricbloodflowvolumeelevated;gastricpHiincreasedsignificantlyat2hours;thelevelofmucosalMDAdecreasedat4hours,theactivityofNa+-K+-ATPaseincreasedandthelacticacidlevelintheportalveindecreasedsignificantlycomparedtotheHSgroup.Conclusions:Afterhemorrhagicshockresuscitation,cisapridecontainedthefollowingfunctions,1)promotinggastricemptying,2)increasingthebloodflowofgastricbloodflowvolumeandgastricpHi,3)depressingthelacticacidconcentrationoftheportalveinandimprovingMDAvolumeandNa+-K+-ATPaseactivityofgastricmucosa.Itsuggeststhataftercomplementingeffectivecirculatingbloodvolumeforhemorrhagicshockresuscitation,earlyuseofcisaprideforgastricmotilityishelpfulforanimprovementoflastingischemiaandhypoxiainstomach.

简介：Thispaperintroducesthecurativeeffectson142cases(278eyes)ofAfricanepi-demichemorrhagicconjunctivitistreatedbyacupuncture,and128(90.1%)cases(250eyes)ofallpatientshavebeenimproved.Threegroupswereclinicallyobservedinthepresentreport.Therewere55cases(104eyes)inthefirstgroupforbodyacupuncture,ofwhich46(83.6%)caseswereeffec-tivelytreated;Inthesecondgroupforauricularacupuncture,34(91.6%)cases(68eyes)outof37cases(74eyes)wereimproved;Therewere50cases(100eyes)inthethirdgroupforboyauricular-acupuncture,amongwhich48(96.0%)cases(96eyes)wereimproved.Thecurativeeffectsofthethirdgroupwerebetterthanthoseoftheothertwogroups.

简介：TheZ10andZ37strainsofhemorrhagicfeverwithrenalsyndrome(HFRS)virusandtheMongoliangerbil(Merionsunguiculatus)kidneycellswereusedtopreparetheinactivatedbivalentvaccine.AphaseⅡclinicaltrialuseofthisvaccinewasmadein750Chinesevolunteers.Theresultsshowedthatthesidereactionratewas2.5%andthesero-conversionrateofneutralizingantibodiesagainstHantaanandSeoulvirusesintheinoculatedvolunteerswere87.6%and96.3%respectively.

简介：Inordertoelucidatethemolecularandimmunologicalmechanismsaswellasthepathogenesisofhemorrhagicfeverwithrenalsyndrome(HFRS),theCD8^+cytotoxicTlymphecytes(CTL)clonewasestablisheddirectlyfromperipheralbloodmononuclearcells(PBMC)ofpatientswithHFRS.TheactivitiesofCTLweredetectedasusualwithEBV-transformedlymphoblastoidcellline(BLCL)astargetcells.TheresultsshowedthattheCTLclonecouldrecognizedandkilledthetargetcellswithspecificityofnucleocapsidproteinofHantaanvirus(HTNVNP)withthecytotoxicitypercentagesof50.2%,25.4%and39.0%respectively.TheseresultsdemonstratedthattheantigenicepitopesofHTNVNPmainlylocatedontheC-temainaloftheviralnucleocapsidprotein.