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简介：Homeoboxtranscriptionfactorsparticipateinthegrowthanddevelopmentofplantsbyregulatingcelldifferentiation,morphogenesisandenvironmentalsignalresponse.Torevealthefunctionsofthesetranscriptionfactorsinrice,weconstructedtheRNAivectorsofOsHox9,amemberofhomeoboxfamily,andanalyzedthefunctionofOsHox9usingreversegenetics.TheplantheightandtilleringnumberofRNAitransgenicplantsdecreasedcomparedwiththoseofwild-typeplants.Reversetranscription-polymerasechainreactionanalysisshowedthatOsHox9expressionreducedinthetransgenicplantswithphenotypicvariance,whereasthatinthetransgenicplantswithoutphenotypicvariancewassimilartothatinthewild-typeplants.ThisresultsuggeststhatthephenotypesofthetransgenicplantswerecausedbyRNAieffects.Thetissue-specificityofOsHox9expressionindicatedthatitwasexpressedindifferentorgans,withhighexpressioninstemapicalmeristemandyoungpanicles.SubcellularlocationofOsHox9demonstratedthatitwaslocalizedonthecellmembrane.

简介：Prestackreversetimemigration（RTM）isanaccurateimagingmethodofsubsurfacemedia.TheviscoacousticprestackRTMisofpracticalsignificancebecauseitconsiderstheviscosityofthesubsurfacemedia.OneofthestepsofRTMissolvingthewaveequationandextrapolatingthewavefieldforwardandbackward;therefore,solvingaccuratelyandefficientlythewaveequationaffectstheimagingresultsandtheefficiencyofRTM.Inthisstudy,weusetheoptimaltime-spacedomaindispersionhigh-orderfinite-difference（FD）methodtosolvetheviscoacousticwaveequation.Dispersionanalysisandnumericalsimulationsshowthattheoptimaltime-spacedomainFDmethodismoreaccurateandsuppressesthenumericaldispersion.Weusehybridabsorbingboundaryconditionstohandletheboundaryreflection.Wealsousesource-normalizedcross-correlationimagingconditionsformigrationandapplyLaplacefilteringtoremovethelow-frequencynoise.NumericalmodelingsuggeststhattheviscoacousticwaveequationRTMhashigherimagingresolutionthantheacousticwaveequationRTMwhentheviscosityofthesubsurfaceisconsidered.Inaddition,forthewavefieldextrapolation,weusetheadaptivevariable-lengthFDoperatortocalculatethespatialderivativesandimprovethecomputationalefficiencywithoutcompromisingtheaccuracyofthenumericalsolution.