简介：

简介：FastPlant(Brassicarapa,Cruciferae)leaftissuefixedinglutaraldehyde-acroleinandpost-fixedinos-mium,wasexaminedforresponsetoseveraleasily-preparedheavymetalstains.Leadanduranium,separatelyandincombination,gavetypicalresultsacrossthespectrumofcellorganellets.Asasinglestainfollowingosmium,bismuthproducedimagesseeminglyequivalenttoleadanduranium.Phosphotungsticacidproducedverygoodmembranedelineationbutproducedawashed-outbackgroundimagesimilartothatfromleadstaining.Carbohydratecompoundswereespeciallyresponsivetoruthenium;thecytoplasmandthematrixofallorganelleswerealsostainedverywell.Theprocedureswerenomoredemandingthantraditionalstainingmethodsandmaybeeasilyusedinresearchandteaching.FastPlantmaterialsareareliable,quickandeasysourceoflivingmaterial.

简介：FastPlant(Brassicarapa,Cruciferae)leaftissuefixedinglutaradehyde-acroleinandpost-fixedinosmium,wasexaminedforresponsetoseveraleasilypreparedheavymetalstains.Leadanduranium,separatelyandincombination,gavetypicalresultsacrossthespectrumofcellorgeanelles.Asssinglestainfollowingosmium,bismuthproducedimagesseeminglyequivalenttoleadanduranium.Phosphotungsticacidproducedverygoodmembranedelineationbutproducedawashed-outbackgroundimagesimilartothatfromleadstaining.Carbohydratecompoundswereespeciallyresponsivetoruthenium;thecytoplasmandthematrixofallorganelleswerealsostainedverywell.Theprocedureswerenomoredemandingthantraditionalstainingmethodsandmaybeeasilyusedinresearchandteaching.FastPlantmaterialsareareliable,quicknandeasysourceoflivingmaterial.

简介：AdoubleimmunocytochemicalstainingmethodwasusedtodemonstrateHBsAgandHBcAgsimultaneouslyinthesamesectionsof58hepatocellularcarcinomas.SeveraltumorcellscontainedbothHBsAgandHBcAg.TheseresultsdemonstratethathepatitisBvirus(HBV)DNAintumorcellscansimultaneouslyexpressbothHBsAgandHBcAgandsuggesttheproductionofacompletevirusbythesecells.CytoplasmicmembranousHBcAgwasfoundnotonlyinwelldifferentiatedhepatocellularcarcinomabutalsoinmoderateandpoorlydifferentiatedhepatocellularcarcinoma.ThelocalizationofHBcAgintumorcellsappearstobethesameasthatofsurroundingnontumorouslivertissue.

简介：ObjectivesTodevelopasimple,accurateandreproduciblemethod,whichcombinesmacroandhistopathologicaltechniquesfordeterminingthedegreeoflipiddepositioningeneticallymodifiedmice.MethodTheentireaortasfromC57BL/6,ldlr-/-andapoE-/-micewerestainedwithSudanⅣusingeitherinvivoperfusionortraditionalinvitroenfacestainingtechniques.Histologicalsectionsofaorticrootandheartswereembeddedintissuefreezingmediumandcutwithacryostat,thenstainedwithOilRedO.Thecalculatedaorticrootareabasedontheaorticrootcircumferencewasusedtoreducemeasurementerrors.ResultsTheinvitroenfacestainingcanstainallfat,whichincludetheadventitialtissuearoundaorta.Howevertheinvivoperfusionstainingcanspecificallystainthefattydepositioninsideofaorta.Bothentireaortaandaorticrootsectionstainingshowedthattherewasahighlysignificantincreaseinfattydepositionintheaortasofthegeneticmodifiedmice.Althoughallmicegeneticbackgroundwassame,theapoE-/-micehadlargeratheroscleroticlesionsthanldlr-/-mice.ConclusionsThenewinvivoperfusionmethodismoreaccuratethantheinvitroenfacemethod.Thecombinationofthesemacroandmicroscopictechniquesovercomestheshortcomingsoftheearlierpublishedmethodswhicharegenerallylimitedtothemeasurementoffattyredstainingareasonly,neglectingnon-specificadventitialfatstainingaroundaortaandaorticrootsectiontissuedistortion.