简介：目的探讨S波段高功率微波（Sbandhighpowermicrowave,S-HPM）辐射对大鼠睾丸Toll样受体（Toll-likereceptor,TLR）表达的影响。方法60只雄性Wistar大鼠经0mW/cm2、30mW/cm2和100mW/cm2的S-HPM辐射20min,通过TUNEL方法检测辐射后6h、3d、7d和14d睾丸生精细胞的凋亡。采用Real-timePCR和Westernblotting检测辐射后睾丸组织TLR2、TLR3、TLR4和TLR5的mRNA和蛋白水平的表达变化。结果经30mW/cm2和100mW/cm2的S-HPM辐射后,生精细胞凋亡率随时间延长而增加,100mW/cm2组重于30mW/cm2组（P〈0.05）;S-HPM辐射后6h、3d和7d可见TLR2-5mRNA表达不同程度的增加（P〈0.05,P〈0.01）;辐射后7d可见TLR2-5蛋白表达的增加,100mW/cm2与30mW/cm2组相比无显著差异。结论S-HPM辐射可致睾丸生精组织凋亡,TLR2-5的表达上调,参与了微波辐射致生精细胞凋亡的病理生理过程。

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简介：摘要目的评价数字心电图机ECG-1230S的自动测量和诊断功能及临床应用价值。方法入选受试者100例，分别用惠州科美思医用仪器有限公司的数字心电图机ECG-1230S和武汉中旗的数字式多道心电图机iMAC120对受试者进行对照试验，对比分析4个心电测量参数和诊断结果。结果数字心电图机ECG-1230S和武汉中旗心电图机所测量的4个心电参数的数据无明显差异，一致性较好，心电诊断符合率达90%。结论数字心电图机的ECG-1230S的性能与武汉中旗产品基本等效，值得应用于临床推广。

简介：Genisteiniseffectiveagainstamyloid-βtoxicity,buttheunderlyingmechanismsareunclear.Wehypothesizedthatgenisteinmayprotectneuronsbyinhibitingthemitochondrialapoptoticpathway,andtherebyplayaroleinthepreventionofAlzheimer'sdisease.AratmodelofAlzheimer'sdiseasewasestablishedbyintraperitonealinjectionofD-galactoseandintracerebralinjectionofamyloid-βpeptide(25–35).Inthegenisteintreatmentgroups,a7-daypretreatmentwithgenistein(10,30,90mg/kg)wasgivenpriortoestablishingAlzheimer'sdiseasemodel,for49consecutivedays.Terminaldeoxyribonucleotidyltransferase-mediateddUTPnickendlabelingassaydemonstratedareductioninapoptosisinthehippocampusofratstreatedwithgenistein.Westernblotanalysisshowedthatexpressionlevelsofcapase-3,Baxandcytochromecweredecreasedcomparedwiththemodelgroup.Furthermore,immunohistochemicalstainingrevealedreductionsincytochromecandBaximmunoreactivityintheserats.MorriswatermazerevealedasubstantialshorteningofescapelatencybygenisteininAlzheimer'sdiseaserats.Thesefindingssuggestthatgenisteindecreasesneuronallossinthehippocampus,andimproveslearningandmemoryability.Theneuroprotectiveeffectsofgenisteinareassociatedwiththeinhibitionofthemitochondrialapoptoticpathway,asshownbyitsabilitytoreducelevelsofcaspase-3,Baxandcytochromec.

简介：腋臭医学上称之为"局部性臭汗症",是一种常见病,夏季时症状最明显,尤其令许多中青年男女患者产生自卑、孤僻消沉等不良情绪,严重影响工作生活.而以手术切除根治效果佳.自2002年初至2003年底我院共以"S"形切口修剪皮瓣法腋臭根治术治疗了92例患者,术后疗效好,报告如下:

简介：摘要目的探索不同乙肝标志物适用对象。方法对我院2009年1月至2009年10月2012例体检和乙肝患者PreS1抗原和其他相关项目结果进性回顾性分析。结果体检人群中HBsAg阳性率为8.3%，而“大三阳”仅为1.2%，PreS1抗原阳性率为1.4%。在HBV-DNA超过103copies/ml时，HBeAg特异性为75.6%，灵敏度为84.4%，而PreS1抗原特异性为57.6%，灵敏度为86.7%。结论PreS1抗原与HBeAg有一定的互补性，但可能存在过高假阳性。健康体检人群可以只查肝表面抗原（HBsAg），PreS1抗原应根据表面抗原的结果而定；而HBeAg仍然是评估慢性乙肝复制的常用指标，但针对HBeAg阴性患者有必要进行PreS1抗原检查。但是PreS1抗原检查也不能取代HBeAg。

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简介：AbstractPurpose:The poor prognosis in patients with floating knee injuries is mainly contributed to articular involvement (Fraser's type II). This study aims to evaluate and compare the functional outcomes among different Fraser's type II floating knee injuries after surgical management.Methods:Twenty-seven patients with Fraser's type II floating knee injuries (54 fractures) between September 2014 and December 2015 were enrolled prospectively in this study and were distributed according to Fraser's floating knee classification into three different groups as type IIA (ipsilateral femoral shaft and tibial intra-articular involvement, n = 11), type IIB (ipsilateral tibial shaft and femoral intra-articular involvement, n = 9) and type IIC (both femoral and tibial intra-articular involvement, n = 7). The differences among the groups were evaluated and compared. The functional outcomes of these injuries at one year were analyzed using Knee Injury and Osteoarthritis Outcome Score (KOOS) which covers 5 subscales of pain, other symptoms, activities of daily living, sports and recreation, and quality of life. The result was also compared with standardized age-sex matched healthy population using paired samples t-test.Results:All the patients were male, and the injury mechanism was solely roadside accident. The mean age was 29.8 years and injury severity score 17.9 (comparable in all the three groups). Most injuries were observed on the right side (20 cases, 74.1%). Based on paired samples t-test, the KOOS score of patients with Fraser's type IIA was found to be better than that of type IIB and type IIC. Compared with the reference age-sex matched control group, patients with Fraser's type IIB and IIC fractures had significantly lower mean score in all KOOS subscales (all p < 0.01). However, Fraser's type IIA only revealed significant difference regarding the subscales of activities of daily living (p < 0.0001), sports and recreation (p < 0.0001), and quality of life (p < 0.0001).Conclusion:The results of this study show that patients with Fraser's type IIA fractures had a better functional outcome as compared to those with type IIB and IIC fractures. This might be due to the open intra-articular involvement of the distal femur of the latter two fracture types.

简介：Addison’s病，全称为慢性原发性肾上腺皮质功能低下症，是由Addison最先描述并予以报道。本病最常见的病因为肾上腺结核和自身免疫性肾上腺炎，其他病因有肾上腺皮质受损等。在20世纪50年代以前，结核是引起Addison’s病的最常见原因，占70％～80％。随着结核发病率的下降和诊治水平的提高，据文献报道，目前在欧美国家，自身免疫性肾上腺炎已成为Addison’s病的首要病因，约占75％～80％，而结核引起者则已降至20％。而目前在我国，结核仍是引起Addison’s病的最常见原因。因此，当有Addison’s病的临床表现时，我们首先应想到肾上腺结核存在的可能。

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简介：摘要目的探索“6S”管理在医院现场管理中的的可行性和有效性。方法在全院37个科室开展“6S”管理活动，对实施“6S”管理模式前、后3个月护理综合质量和患者满意度进行比较。结果实施6S管理后，护理综合质量和患者满意度较实施前明显改善。结论医院实施“6S”管理模式，不仅改善了医院环境和物品仪器管理，在提高工作质量和效率、提升医护人员和患者的综合满意度方面也起到了重要作用，该管理模式提供医院高效现场管理思路，值得推广和应用。

简介：摘要目的探讨5S在医院感染控制管理中的作用。方法2015年7月开始将5S管理工具运用到医院感染控制管理中。结果5S管理前后科室清洁消毒合格率由46.50%升到87.33%、无菌物品储存合格率由64.00%升到100.00%、医疗废物分类合格率由77.00%升到94.50%、输血袋规范处置率由20.00%上升至71%、污衣存放合格率由19.42%上升至75.47%。结论5S管理能加强医院感染环节管理，可营造整齐、清洁、美观、安全、舒适的工作环境,减少浪费,减少库存,提高工作效率,增加患者的安全感,确保了医疗质量和医疗安全1，增强患者对医院的满意度,提高医院在医疗市场中的竞争力。

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简介：Tosupportthescientificbasisforrapididentificationofpathogenicbacteriaandotherstud-ies,thesequencesofhsp60geneinmajor34speciesof16genusofpathogenicbacteriaweresearchoutinGenBankandaproperpairofuniversaldegenerateprimerwasdesignedbymeansofthemolecu-larbiologicalsoftwawePrimer5.0andOligo6.0.ThisprimerwasthenusedinthePCRamplification,andthehsp60genefragmentsoftheselectedpathogenicbacteriacouldbeamplifiedusingthisdegener-ateprimer.Bywayofbioinformationalanalysis,theconservation,variationandtheinterspeciesphylo-geneticrelationsofthehsp60genesequencewereanalysed.Fromtheresultsofthecomparativestudyonsequences,itwasdemonstratedthatthehsp60genewascharacterizedbyconservationandvaria-tion,inwhichtheconservedandmutantregionsco-existedandseparatelydistributedwithmanysmallmutantregionsdistributedamongtheconservedregions,justlikethemosaic.Thephylogenetictreeamongdifferentpathogenicbacteriadrawnfromthehsp60geneanalysiswasprovedtobeconsistentwiththosefrom16SrRNAand23SrRNA.Itisconcludedthatthesequencedistributionofhsp60genewouldprovideasolidbasisfortherapididentificationofpathogenicbacteriaandthedevelopmentofadiagnosticmicroarray.

简介：<正>Paget’s病也称变形性骨炎或畸形性骨炎,是一种以慢性进行性原因不明的局部骨组织病变为特点的疾病。主要表现为骨的过度吸收,同时伴有骨的过度形成,但新生骨失去正常形态结构。本病以欧美国家发病率较高,我国发病率较低。吴恩惠等曾有报告,而颅

简介：Cancerisamajorhealthconcernandleadingburdenoneconomyworldwide.Anincreasingeffortisdevotedtoisolationanddevelopmentofplant-deriveddietarycomponentsaseffectivechemo-preventiveproducts.Phytochemicalcompoundsfromnaturalresourcessuchasfruitsandvegetablesareresponsiblefordecreasingtheriskofcertaincancersamongtheconsumingpopulations.Apigenin,aflavonoidphytochemicalfoundinmanykindsoffruitsandvegetables,hasbeenshowntoexertsignificantbiologicaleffects,suchasanti-oxidant,anti-inflammatoryandmostparticularlyanti-neoplasticproperties.Thisreviewisintendedtosummarizethemostrecentadvancesintheanti-proliferativeandchemo-preventiveeffectsofapigeninindifferentcancermodels.Analysisofthedatafromthestudiedcancermodelshasrevealedthatapigeninexertsitsanti-proliferativeeffectsthroughmultipleandcomplexpathways.Thisguidedustodiscoversomecontroversialresultsabouttheexactroleofcertainmolecularpathwayssuchasautophagyintheanticancereffectsofapigenin.Further,therewerecumulativepositiveevidencessupportingtheinvolvementofcertainpathwayssuchasapoptosis,ROSandDNAdamageandrepair.Apigeninpossessesahighpotentialtobeusedasachemosensitizingagentthroughtheup-regulationofDR5pathway.Accordingtothesepreclinicalfindingswerecommendthatfurtherrobustunbiasedstudiesshouldconsiderthepossibleinteractionsbetweendifferentmolecularpathways.

简介：摘要目的总结Mile’s术腹膜外隧道式造口的护理。方法对2004年3月-2010年3月133例直肠癌及肛管癌行Mile’s术腹膜外隧道造口的患者做好术前护理心理护理及造口的护理。结果133例患者住院期间未发生造口并发症，提高患者术后的生活质量。结论腹膜外隧道式造口能降低造口并发症的发生，科学有效的护理能提高术后疗效，提高患者对医疗护理服务的满意度。

简介：摘要目的探讨运用克氏针预固定、重建钢板最终固定的改良术式治疗锁骨骨折。方法2012～2014年间采用切开复位改良术式重建钢

简介：<正>标记具有手性碳原子的化合物的构型,通常是根据IUPAC确定的R/S构型标记法来判断其构型的。该法的难点在于随着分子立体结构的不同表示法,观察者要相应改变观察方向以确定其构型。对于一对对映体,观察者要从相反的两个方向去观察判断。如乳酸对映体的构型标记(见图1),若用图2的表示式,观察者的观察位置就很难确定了。由于观察者没有固定的观察方向,这对于缺乏立体概念的学生来说,常感困惑不解,难于掌握。特别是