简介:[Co3(1,2-S2C6H4)3(PPh3)3][CoBr3(DMF)].sol(1,sol=CHCl3,O(C2H5)2,H2O)wasobtainedfromthereactionofCoBr(PPh3)3withNa2(S2C6H4)inchloroform.TheCo3coreinthecationof1exhibitsametal-metalbondedisoscelestriangle,inwhichthetwolongerCo-CobondsarebothbridgedbyS2C6H4ligandsontwosidesofthetriangleplanerespectively,whilethebottomshortCo-CobondisbridgedbythethirdbidentateS2C6H4ligand.Aseriesofpolynu-clearcobaltclustercompoundswithphosphine,thiolateand/orsulphurligandswerepreparedbylowoxidationstateCo+withthiolatesinorganicsolvents.Thesetri-,tetra-,hexa-,heptanuclearclustercompounds1-8withvarioustypesofcrystalstructurescanbeviewedasthecondensedpolynuclearcobaltcomplexesthatthecobaltatomframeworkswithsulphurbridgedwerebuiltthroughthesmalltriangularunitsof[Co3S3nL3](n=1,2)withorwithout[CoL](L=PR3,Br,Cl,5-C5H5)fragments.
简介:目的采用核磁(1H.NMR)代谢组学技术初步探讨四氯化碳(CCl4)致大鼠急性肝损伤后血清代谢物变化规律。方法SD大鼠随机分为空白、模型两组,模型组ip40%CCl4植物油溶液造成急性肝损伤模型,空白组注射等体积的植物油,造模24h后股动脉取血,常规肝脏病理切片,取血前12h禁食不禁水。比色法测定血清丙氨酸氨基转移酶(ALT)含量,600MHzNMR波谱仪分析血清中小分子代谢物代谢轮廓,并对数据进行多元统计分析。结果模型组中ALT的含量显著升高,肝脏病理切片显示模型组大鼠的肝组织内形成很多空泡,肝细胞变大,坏死严重,有出血和大量中性粒细胞浸润:血清1H-NMR代谢图谱中共鉴定了20个代谢物,并确认了6个与CCl4致肝损伤相关的标志物。结论常规血生化指标和肝病理切片结果与代谢组学的多元统计分析结果相一致,且代谢组学可发现引起肝损伤的代谢标志物。
简介:1IntroductionLetA∈Cn×n,B∈Cn×n.WesayBisasquarerootofAifA=B×Bi.e.A=B2.Itiswell-knownthatanysymmetricpositivedefinitematrixexistsoneandonlyonesquarerootwhichisasymmetricpositivedefinitematrix,too(e.g.see[5]).Higham[4]studiedcarefullytherelationofarealnonsingularmatrixbetweenitsrealsquarerootsanditseigenvalues.AlefeldandSchneider[1]pointedoutthatforanynonsingularM-ma-trixthereisoneandonlyoneM-matrixasitssquareroot.Inthispaper,westudyona
简介:AbstractObjective:The aim of this work was to explore the feasibility of in vivo and non-invasive monitoring of deuterium/hydrogen (2H/1H) exchange at the metabolic level upon exposure to heavy water (2H2O).Methods:The healthy female mice were randomly assigned to two groups after day 0 when both mice received standard drinking water. The treated mouse was fed with 2H2O (80%, v/v) and the control mouse fed with standard drinking water (H2O) over next 13 days. Real-time mass spectrometric analysis of volatile metabolism emitted through breathing and the skin was performed on days 1, 2, 3, 10, 12, and 13. Animal experiment was approved by the Laboratory Animal Ethics Committee of Jinan University (approval No. 20161117163322) on October 29, 2021.Results:We observed a replacement of 1H by 2H in 52 mass spectral features (60 2H/1H isotopologue pairs) for the mouse fed with 2H2O, but not for the control mouse. These included pyruvic acid and lactic acid, lysine and methyl-lysine as well as short-chain fatty acids comprising acetic acid, propionic acid, butyric acid and valeric acid.Conclusion:Secondary electrospray ionization-high resolution mass spectrometry allows monitoring in vivo2H-incorporation of metabolites in a non-invasive and real-time setup and opens new opportunities to use 2H tracing to extend current metabolic studies, especially those with a focus on anaerobic glycolysis, lysine methylation and gut microbiome via monitoring of short-chain fatty acids.