简介:UsingXenonlamplightstoovercomethestronginterferencefromtheweldingarc,acomputer-basedsystemisdevelopedtosenseandvisualizethemetaltransferinGMAW.Thissystemcombinesthrough-the-arcsensingoftheweldingcurrentandarcvoltagewithhighspeedimagingofthemetaltransfer.Itcansimultaneouslydisplaythemetaltransferprocessesandwaveformsofelectricalweldingparametersinreal-timeThemetaltransfervideosandwaveformsofelectricalweldingparameterscanberecorded.Metaltransfersundervariousweldingconditionshavebeeninvestigatedwiththesystemdeveloped.
简介:Abicentralpolymer-supportedphasetransfercatalyst,polystyrene-supportedpolyethyleneglycolandpyridiniumsalt(PS-Py-PEG-400),synthesizedwithchloromethylatedpolystyreneassupporteronwhichPEGandpyridiniumsaltwereimmobilizedsuccessively.ItscatalyticactivitywastestedforthereactionofsolidpotassiumacetateandbenzylbromidebyGCanalysis.ItwasfoundthatthebicentralcatalystperformedhigheractivitythanthemonocentralPS-PEG-400andPS-Py.
简介:ObjectiveTopresentanexperimentalmethodthatallowsisolationofgreaterepithelialridge(GER)andlesserepithelialridge(LER)cellsfrompostnatalratcochleaeusingacombinatorialapproachofenzymaticdigestionandmechanicalseparationandtoinvestigatearetrovirus-mediatedgenetransfertechniqueforitspossibleutilityinimmortalizationoftheGERandLERcelllines,inanefforttoestablishaninvitromodelsystemofhaircelldifferentiation.MethodsGERandLERcellsweredissectedfrompostnatalratcochleaeandimmortalizedbytransferringtheSV40largeTantigenusingaretrovirus.Theestablishedcelllineswereconfirmedthroughmor-phologyobservation,immunnocytochemicalstainingandRT-PCRanalysis.TheHath1genewastransferredintothecelllinesusingadenovirus-mediatedtechniquestoexploretheirpotentialtodifferentiateintohaircells.ResultsTheestablishedcelllineswerestablymaintainedformorethan20passagesanddisplayedmanyfeaturessimilartoprimaryGERandLERcells.Theygrewinpatchesandassumedapolygonalmorphology.ImmunostainingshowedlabelingbySV40largeTantigenandIslet1(aspecificmarkerforGERandLER).AllpassagesofthecelllinesexpressedSV40largeTantigenonRT-PCRanalysis.Thecellsalsoshowedthecapabilitytodifferentiateintohaircell-likecellswhenforcedtoexpressHath1.ConclusionRetrovirus-mediatedgenetransfercanbeusedinestablishingimmortalizedprogenitorhaircelllinesinnewbornrat,whichmayprovideaninvaluablesystemforstudyinghaircelldifferentiationandregenerationfornewtreatmentofsensoryhearinglosscausedbyhaircellloss.
简介:三个米饭变化,Zhonghan3,Shanyou63和Aizizhan,当在检测微分活跃甲基的材料骑车并且响应干旱应力转移相关基因表示,被使用。实验被在干旱的条件下面的微分显示器技术与10%PEG6000答案模仿了的基因薄片和mRNA执行。结果显示甲基周期能在Zhonghan3和Shanyou63的叶子被激活,但是在干旱应力下面在Aizizhan的叶子禁止了。而且,干旱应力能导致很多methyltransferase基因的表示,特别Rubisco蛋白质methylation的抄写联系了基因,它是有益的因为Rubisco蛋白质氧化和降级的预防,和干旱应力能禁止DNAmethyltransferase基因和histonemethyltransferase基因的抄写。这结果证实活跃甲基骑车并且转移相关基因涉及米饭干旱抵抗。
简介:Cross-bordertransferofthecompanyseatformorethandecadewasandstillishotandunresolvedtopicinEUCompanyLaw.FreedomofestablishmentisindisputablecornerstoneofEUcompanylawandInternalMarketenshrinedinArticles49and54oftheTreatyontheFunctioningoftheEuropeanUnion(TFEU)butstillthecompany'scrossbordermobilityisunsolved.ThecompanytransferoftheseatiscloselyconnectedwithdifferentMemberStateslegaltraditioninrelationwiththetwodifferent"seattheories"thatapply.BythepracticeoftheEuropeanCourtofJusticebothseattheories"therealseattheory"and"theincorporationtheory"areproclaimedasinlinewiththeEUlaw.Nevertheless,theprocessofcrossbordertransferoftheseatoccursascompellingproblemforthecross-bordercompanymobility.InmostMemberStatesatthemomentthecrossbordertransferofthecompanyseatrequiresthewinding-upofthecompanyandestablishingthenewoneintheotherMemberState.Thisprocedureleadstothelossoflegalandbusinesscontinuityandnationalapproachestothisissuediffer.
简介:Horizontalgenetransfer(HGT)haslongbeenconsideredasaprincipalforceforanorganismtogainnovelgenesingenomeevolution.Homologysearch,phylogeneticanalysisandnucleotidecompositionanalysisarethreemajorobjectiveapproachestoarguablydeterminetheoccurrenceanddirectionalityofHGT.Here,21genesthatpossessthepotentialtohorizontaltransferwereacquiredfromthewholegenomeofMagnaporthegriseaaccordingtoannotation,amongwhichthreecandidategenes(correspondingproteinaccessionnumbersareEAA55123,EAA47200andEAA52136)wereselectedforfurtheranalysis.AccordingtoBLASThomologyresults,wesubsequentlyconductedphylogeneticanalysisofthethreecandidateHGTgenes.Moreover,nucleotidecompositionanalysiswasconductedtofurthervalidatetheseHGTs.Inaddition,thefunctionsofthethreecandidategenesweresearchedinCOGdatabase.Consequently,weconcludethatthegeneencodingproteinEAA55123istransferredfromClostridiumperfringens.AnotherHGTeventisbetweenEAA52136andacertainmetazoan'scorrespondinggene,butthedirectionremainsuncertain.Yet,EAA47200isnotatransferredgene.
简介:Thegrowthandcollapsebehaviorsofasinglecavitationbubblenearaheatedwallanditseffectontheheattransferarenumericallyinvestigated.Thepresentstudyisdesignedtorevealthemechanismofcavitationenhancedheattransferfromamicroscopicperspective.Inthesimulation,thetime-dependentNavier-Stokesequationsaresolvedinanaxisymmetrictwo-dimensionaldomain.Thevolumeoffluid(VOF)methodisemployedtotracktheliquid-gasinterface.Itisassumedthatthegasinsidethebubbleiscompressiblevapor,andthesurroundingliquidisincompressiblewater.Masstransferbetweentwophasesisignored.Thecalculatedbubbleprofileswerecomparedtotheavailableexperimentaldata,andagoodagreementwasobtained.Then,therelationshipamongbubblemotion,flowfieldandsurfaceheattransfercoefficientwasanalyzed.Onthisbasis,theeffectsofsuchfactorsastheinitialdistancebetweenthebubbleandthewall,theinitialvaporpressureandtheinitialbubblenucleussizeontheheattransferenhancementarediscussed.Thepresentstudyishelpfultounderstandtheheattransferphenomenoninpresenceofcavitationbubbleinliquid.
简介:Inthispaper,itisdiscussedandresearchedonthetransferemploymentissuesofruralsurpluslaborforceintheprocessofthenew-typeurbanizationconstructionpenetratingly.FacingthetransferemploymentproblemsofruralsurpluslaborforceencounteredintheprocessofurbanizationconstructioninChina,itispointedoutthatthefundamentalwaytosolvethetransferemploymentproblemsofruralsurpluslaborforceeffectivelyisthatthenew-typeurbanizationconstructionispromotedsteadilywiththecoordinateddevelopmentofurbanization,industrialization,agriculturalmodernization,andthebasicguaranteetosolvethetransferemploymentproblemsofruralsurpluslaborforceisthatthesupportingpoliciesandmeasuresofurbanizationarelaunchedandimprovedgraduallyinthecoordinateddevelopment.
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简介:Thefilmdiffusionmass-transferprocessofadsorptionofphenolonmacroporouspolystyreneresinwasinvestigatedindetail.InordertorevisetheBoydfilmdiffusionkineticsequation,theout-surfacestructureofthemacroporousresinandthatofgel-typeion-exchangeresinwascomparedandthenewfilmdiffusionequationwasalsosuggested.Theseresultsshowedthatthefilmdiffusionwasinfluencedbyporosityofthemacroporousresingreatly,whichdifferedfromthefilmdiffustionbehaviorofion-exchangeresinobviously.
简介:Inthepresentwork,theheattransferstudyfocusesonassessmentoftheimpactofbedtemperatureonthelocalheattransfercharacteristicbetweenafluidizedbedandverticalrifledtubes(38mm-O.D.)inacommercialcirculatingfluidizedbed(CFB)boiler.Heattransferbehaviorina1296t/hsupercriticalCFBfurnacehasbeenanalyzedforGeldartBparticlewithSautermeandiameterof0.219and0.246mm.TheheattransferexperimentswereconductedfortheactiveheattransfersurfaceintheformofmembranetubewithalongitudinalfinatthetubecrestunderthenormaloperatingconditionsofCFBboiler.AheattransferanalysisofCFBboilerwithdetailedconsiderationofthebed-to-wallheattransfercoefficientandthecontributionofheattransfermechanismsinsidefurnacechamberwereinvestigatedusingmechanisticheattransfermodelbasedonclusterrenewalapproach.ThepredictedvaluesofheattransfercoefficientarecomparedwithempiricalcorrelationforCFBunitsinlarge-scale.
简介:Ethanol-waterbinarymixtureswith7differentmolefractionsofethanolrangingfrom0to1wereadoptedastestingliquidsintheexperiment.Theverticalheatingtubewasinsertedinporousmatrixcomposedoffivewellsortedglassbeadswhisediametersrangefrom0.5to4.3mm.Duetotheeffectofcomposition,thetrendofcombinationofvaporbubbleswasreduced.resultingintheincreaseofpeakheatfluxofbinarymixture,Withtheincreaseofethanolmolefraction,0.5mmdiameterbeadofpeakheatfluxofbinarymixture.withtheincreaseofethanolmolefraction.0.5mmdiameterbeadhadlowervalueofpeakheatflux,whileforpureliquidthecriticalstateisdifficulttoappear,withgivendiameterofglassbead,thereexistedanoptimumvalueofmolefractionofethanol,whichwasdecreasedwiththeincreaseofbeaddiameter,Adimensionlessheattransfercoefficientwaspredictedthroughtheintroductionofadimensionlessparameterofporousmatrixwhichagreedwiththeexperimentalresultssatisfactorily.
简介:WeproposeaprotocoltoimplementthenonlocalBell-statemeasurement,whichisnearlydeterminatewiththehelpofweakcross-Kerrnonlinearitiesandquantumnon-destructivephotonnumberresolvingdetection.BasedonthenonlocalBell-statemeasurement,weimplementthequantuminformationtransferfromoneplacetoanother.Theprocessisdifferentfromconventionalteleportationbutcanberegardedasanovelformofteleportationwithoutentangledchannelandclassiccommunication.
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简介:AnewkindofsimpleandflexibleCO_2weldingsystemwasdevelopedtocarryoutwaveformcontrol.ThesystemconsistedofIGBTinverter,PWMcircuitandmicroprocessorunit(MPU),inwhichtheoutputcurrentofconstantcurrent(CC)powersupplycouldbechangedaccordingtotransientphysicalstate,andthevariabledownsloperatecontrolcouldbeusedtoensureastableweldingprocess.Theweldingexperimentresultsprovedtheeffectivenessofthiscontrolapproach.