简介:Aseriesof5-aminolevulinicacidanditsalkylestermethanesulfonateswasexploitedtophotodynamictherapy(PDT)ofhumanlymphocyticcells,U-937invitro.ThePDTefficiencyisinfluencedbytheconcentrationandincubationtime.Generally,forALAanditsalkylestermethanesulfonates,thecellsurvivalratedecreasesandtheaccumulationabilityofPpIXincreaseswiththeconcentrationandincubationtime.Wefoundthatthelongercarbonchainmethanesulfonates(C5-S,C6-S,C8-S)exhibitbetterPDTeffectthanALAmethanesulfonate.ThispossiblyprovidesapromisingroutetotheclinicalapplicationofPpIX-mediatedPDTtocancercell.
简介:4,4-Dialkyl-2-oxazolin-5-onesarepreparedbyonestepreactionof2-oxazolin-5-onewiththemagnesiummethylcarbonate(MMC)andalkyIhalides.Itisanovelandconvenientmethodforsynthesisofα,Υ-disubstitutcdα-aminoacidbyhydrolysisof4,4-dialkyl-2-oxazolin-5-ones.
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简介:酸不稳定的硫化物(AVS)在影响二价的猫的毒性起一个重要作用,是众所周知的在缺氧的沉积以内的离子的金属。在学习在Jiaozhou海湾以内从潮滩收集的沉积岩心试样,中国,我们发现AVSconcentration逐渐地与深度增加并且从高潮滩减少到低潮滩区域。我们基于同时提取的金属(SEM)和AVS的臼齿的比率在潮滩评估了化学活动和重金属的简历可获得性。SEM/AVS的价值通常除了表面层在这个区域是不到1,它建议重金属仅仅在表面层举办化学活动。SEM最高度在氧化还原作用层的边界被集中。SEM在整个潮滩有类似的深度分布。低潮滩沉积的Theaeration显示SEM逐渐地经由孔隙水搬到更深的地点。
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简介:ThedynamiccompetititveadsorptionbehaviorsofdifferentbinaryorganicvapormixturesonACF-Psunderdifferentoperationconditonswereinvestigatedbygaschromatographyinthispaper,Thestudiedmixtruesincludedbenzene/toluene,toluene/xylene,benzene/isopropylbenzeneethlyacetate/tolueneandbenzene/ethylacetate.ExperimentalresultsshowthatvariousACF-Ps,aswithACF-W,canremovebothvaporsinbinaryvapormixtureswithover99%ofremovalefficiencybeforethebreakthroughpointofthemoreweaklyadsorbedvapor,Indynamiccompetitiveadsorption,themoreweaklyweakleadsorbedvapornoeonlypenetratesearly,butalsowillbedisplacedanddesorbedconsequentlybystrongeradsorbateandthereforeproducesarollingupinthebreakthroughcurve,TheACF-Pspreparedatdifferenttemperatuershavesomewhatdifferentadsorptionselectivity,Thefeedconcentrationratioofvapros,thelength/diameterratioandthethickofbedhaveeffectoncompetitiveadsorption.Thecompetitiveadsorptionabilityofavaporismainlyrelatedtoitsboilingpoint.Usually,thehighertheboilingpoint,thestrongerthevaporadsorbedonACF-P.
简介:Objective:ToinvestigatethemembranelocalizationfunctionoftheCX26proteinwhenits86thaminoacidisThr,SerorArg,anditsrelationstodeafness.Methods:CX26-GFPproteinwitheitherThr,SerorArgasthe86thaminoacidwasexpressedinmouseSGNcellsviatheGFPfusiontypelentivirusexpressionsystem.Themembranelocalizationofthefusionproteinwasobservedunderafluorescencemicroscope.Results:ThemutatedproteinofCX26T86Swaslocalizedtocellmembraneandformgapconjunctionstructures,showingnodifferencetothewildtypeCX26protein(withThrasthe86thaminoacid).However,thegapconjunctionstructuredisappearedwhenthemutationwasCX26T86A.Conclusion:TheseresultsindicatethattheCX26T86Rmutationmaybeacauseofhearingloss,butCX26T86Sasanon-pathogenicpolymorphismmutationdoesnotaffectfunctionsoftheCX26protein.Theresultsareinaccordancewiththeresultsofclinicalscreening.
简介:Amethodofmulti-beamfemtosecondlaserirradiationcombinedwithmodifiedHF-HNO_3-CH_3COOHetchingisusedfortheparallelfabricationofall-siliconplano-concavemicrolensarrays(MLAs).Thelaserbeamissplitbyadiffractiveopticalelementandfocusedbyalenstodrillmicroholesparallelyonsilicon.AnHF-HNO_3-H_2SO_4-CH_3COOHsolutionisusedtoexpandandpolishlaser-ablatedmicroholestoformmicrolenses.ComparedwiththeHF-HNO_3-CH_3COOHsolution,thesolutionwithH_2SO_4caneffectivelyreducetheetchedsurfaceroughness.ThemorphologiesofMLAsatdifferentlaserpowersandpulsenumbersareobserved.Theimagearrayformedbythesiliconmicrolensesisalsodemonstrated.
简介:Thecruxoftissueengineeringistoconstructhighlyporousthree-dimensionalscaffoldwithdesiredcellsonit.Becausethescaffoldservesasbothaphysicalsupportandanadhesivesubstrateforisolatedcellsduringinvitrocultureandsubsequentimplantation,thematerialsforscaffoldshouldbenon-toxicandbiocompatible,highlyporousandprocessableintodevicesofvariousshapes.
简介:Acidsulfatesoils(ASS)containconsiderableamountsofreducedsulfurcompounds(mainlypyrite)whichproducesulfuricacidupontheiroxidation.ASS-derivedenvironmentaldegradationwidelyoccursinthecoastallowlandsaroundtheworld,especiallyinthetropicalandsubtropicalareas.ThepresenceofASSiuntheSouthChinahasbeenrecognizedbuttheirdistributionmaybelargelyunderestimatedbecausethesoilsurveydataconcerningASSarebasedonunreliablemethodsandtechniques.ASSintheSouthChinahavebeentraditionallyusedforricecultivationandthispracticehasbeenprovedsustainableifappropriateimprovementmeasuresareadopted.Recently,therapideconomicgrowthintheregionhasresultedinintensifiedcoastaldevelopmentwhichfrequentlyinvolvesactivitiesthatmaydisturbASS,Constructionofroads,foundationsandaquaculturepondsmaycausetheexposureofASStoairandbringaboutsevereenvironmentalacidificvation.Thereiscurrentlyinsufficientawarenessoftheproblemsamongtheresearchers,policy-makersandlandmanagersintheSouthChina.MoreattentionmustbepaidtothepossibleASS-dervivedenvironmentaldegradationinordertoensureasustainabledevelopmentofthecoastallowlandsintheSouthChinaregion.
简介:ThisstudysoughttoidentifydifferentiallyexpressedproteinsinSH-SY5Ycellstreatedwithvalproicacid,usingtwo-dimensionaldifferencegelelectrophoresisanalysis.Threeproteinswereunambi-guouslyidentified:theeukaryotictranslationinitiationfactor4Aisoform1andATP6V1B2proteinweredownregulated,whiletheheterogeneousnuclearribonucleoproteinKwasupregulated.Moreover,allthreeproteinsareassociatedwithalteredexpressionduetooxidativestress.Ma-trix-assistedlaserdesorption/ionization-timeofflightmassspectrometryandproteinimmunoblottingassayconfirmedthedifferentialexpressionofeukaryotictranslationinitiationfactor4Aisoform1.Theresultsindicatethatvalproicacidexertsanantioxidationeffectbyregulatingtheexpressionofeukaryotictranslationinitiationfactor4Aisoform1.