简介：在18根细胞质的男无菌的线和11根restorer线之中的基因距离被分析，分子的标记源于收益相关的功能的基因。在父母基因距离和杂种优势之间的关联被分析47联合的表演调查。结果证明基因距离显著地与收益杂种优势被相关(r=0.29*)，然而并非显著地为另外的特点与杂种优势相关，例如有效圆锥花序的数字每植物，种子背景率，1000谷物重量，每圆锥花序和理论产量谷物数。然而，关联系数是那么小的父母基因距离不能被用来预言杂种优势。

简介：

简介：Themainpathophysiologyofcerebralischemiaisthestructuralalterationintheneurovascularunit,coincidingwithneurovascularmatrixdegradation.Resveratrolhasbeenreportedtobeoneofthemostpotentchemopreventiveagentsthatcaninhibitcellularprocessesassociatedwithischemicstroke.Matrixmetalloproteinases(MMPs)hasbeenconsideredasapotentialdrugtargetforthetreatmentofcerebralischemia.Toexplorethis,wetriedtoinvestigatetheinteractionofresveratrolwithMMPsthroughmoleculardockingstudies.At30minutesbeforeand2hoursaftercerebralischemia/reperfusioninducedbyocclusionofthemiddlecerebralartery,40mg/kgresveratrolwasintraperitoneallyadministered.Afterresveratroladministration,neurologicalfunctionandbrainedemaweresignificantlyalleviated,cerebralinfarctvolumewassignificantlyreduced,andnitriteandmalondialdehydelevelsinthecorticalandstriatalregionsweresignificantlydecreased.ThemoleculardockingstudyofresveratrolandMMPsrevealedthatresveratroloccupiedtheactivesiteofMMP-2andMMP-9.Thebindingenergyofthecomplexeswas–37.848672kJ/moland–36.6345kJ/molforMMP-2andMMP-9,respectively.IncaseofMMP-2,Leu164,Ala165andThr227wereengagedinH-BondingwithresveratrolandincaseofMMP-9,H-bondingwasfoundwithGlu402,Ala417andArg424residues.ThesefindingscollectivelyrevealthatresveratrolexhibitsneuroprotectiveeffectsoncerebralischemiathroughinhibitingMMP-2andMMP-9activity.

简介：<正>ZSM-5/MCM-41compositemolecularsievewaspreparedbythenano-assemblingmethod.TheZSM-5molecularsieve,theMCM-41molecularsieve,theZSM-5/MCM-41mechanicalmixtureandtheZSM-5/MCM-41compositemolecularsievewerecharacterizedbyX-raypowderdiffractometry,N2adsorptionisotherms,temperatureprogrammeddesorptionofammoniaandscanningelectronmicroscopyandtheirpropertieswereanalyzed.UsingFCCgasolineasthefeed,activitiesofdifferentmolecularsievesforreducingolefincontentwereinvestigatedinacontinuoushigh-pressuremicro-reactorunitunderthefollowingconditions:areactiontemperatureof400℃,areactiontimeof2h,aweighthourlyspacevelocityof3h-1,andareactionpressureof2.0MPa.TheresultsshowedthattheHMCM-41molecularsievehadlowreactionperformance,andtheHZSM-5molecularsievedemonstratedhigharomatizationactivity,whiletheZSM-5/MCM-41compositemolecularsieveexhibitedabestolefin-reducingperformancebecauseofitshighisomerizationactivityandmoderatearomatizationactivity.Withalargestolefin-reducmgcapabilityandareasonabledistributionofproducts,thecompositemolecularsievewasmoresuitableforFCCgasolineupgradingcomparedtootherthreecatalysts.

简介：ThekeyforriceplantsurvivalunderNaClsaltstressismaintainingahighK+/Na+ratioinitscells.Selectionforsalttolerancericegenotypesbasedonphenotypicperformancealonewilldelayinprogressinbreeding.Useofmolecularmarkersintandemwithphysiologicalstudieswillhelpinbetteridentificationofsalttolerantriceaccessions.EightriceaccessionsalongwiththecheckDongjinwerescreenedusing1/2Yoshidasolutionwith50mmol/LNaClattheseedlingstage.TheaccessionsIT001158,IT246674,IT260533andIT291341wereclassifiedassalttolerantbasedontheirK+/Na+ratios.SeventeenSSRmarkersreportedtobeassociatedwithK+/Na+ratiowereusedtoscreentheaccessions.FiveSSRmarkers(RM8053,RM345,RM318,RM253andRM7075)coulddifferentiateaccessionsclassifiedbasedontheirK+/Na+ratios.BandingpatternoftheaccessionswasscoredcomparedtothebandingpatternofDongjin.ThestudydifferentiatedaccessionsbasedontheirassociationofK+/Na+ratiowithmolecularmarkerswhichareveryreliable.Thesemarkerscanplayasignificantroleinscreeninglargesetofricegermplasmsforsalttoleranceandalsohelpinidentificationofhigh-yieldingvarietieswithbettersalttolerance.Thesalttolerantaccessionscanbetakenforwardintodevelopingbettervarietiesbyconventionalbreedingandexploringgenesforsalttolerance.

简介：烟碱的醋胆素受体(nAChRs)是cys环的成员门ligand的离子隧道(cysLGIC)总科，调停的快synaptic在在昆虫的中央神经系统的胆碱能的传播。昆虫nAChRs是经济地重要的杀虫药剂的分子的目标，例如neonicotinoids和spinosad。在米饭的nAChR基因家庭的鉴定和描述有斑纹的茎borer，Chilosuppressalis，能关于这个重要受体基因家庭提供有益的信息并且为现在和将来的化学控制策略贡献杀虫药剂行动和电阻的分子的模式的调查。我们寻找了我们的C。在本地强风搜索的suppressalistranscriptome数据库使用BombyxmorinAChR序列并且获得了经由反向的抄写聚合酶链反应(RT-PCR)和cDNA的快速的扩大的互补DNA(cDNAs)结束的通常认为的nAChR子单元方法。类似于B。mori，C。suppressalis拥有12个nAChR子单元，包括九种类型和三个类型子单元。量的RT-PCR分析在各种各样的纸巾揭示了nAChR子单元的表示侧面，包括大脑，subesophageal中心，胸的中心，腹的中心，血球，胖身体，前肠，midgut，hindgut并且肾小体小管。发展表示分析在整个C显示出nAChR子单元的清楚的微分表示。suppressalis生命周期。在这研究的nAChR子单元的鉴定将为调查nAChRs在C起的多样的作用提供一个基础。suppressalis并且为为控制农业害虫的化学药品探索特定的目标地点当时sparing有益的种类。

简介：

简介：HavingstudiedthebiomarkercompositionandmaturityofdissolvedhydrocarbonsfromOrdovicianformationwaters,theauthorspresentedmoleculargeochemicalevidenceforthecontroversialoriginofnaturalgasesincentralOrdosBasin.ThedissolvedhydrocarbonsinWellShan12andWellShan78arerelativelyhighinabundanceoftricylicterpane,pregnaneseriesanddibenzothiopheneseriesandlowinPr/Ph(＜2)andhopane/steraneratios,indicatingthesourceinputofmarinecarbonates.Incontrast,thedissolvedhydrocarbonsinWellShan81arefreefromtricyclicterpaneandpregnaneseries,withtracedibenzothiopheneseriesandhighPr/Ph(3.27)andhigherhopane/steraneratios,whicharethetypicalfeaturesofterrestrialorganicmatter.Furthermore,WellShan37andWellShan34arebetweenthetwosituations,havingamixedsourceofmarinecarbonateandterrestrialorganicmatter.Thematurityofbiomarkersalsosupportstheabovesuggestions.Theseresultsareconsistentwiththegeologicalbackgroundandsourcerockdistributioninthisregion.

简介：TheternaryCu(Ⅱ）complexwith2,2′-bipyridyl(bipy)andL-methioninte(L-Met)hasbeensynthesizedandcharacterizedbyelementalanalysis,molarconductivity,UV-Visspectra,IRspectraandpH-potentiometrictitrationmethods.Thestructureofthecomplex[Cu(L-Met)(bipy)(H2O)]ClO4·3/8H2OwascharacterizedbytheX-raydiffractionanalysis.Itcrystallizesinthetriclinicsystem,spacegroupP1withfourmoleculesinaunitcellofdimensions,a=0.7656(2)nm,b=1.3142(3)nm,c=2.0596(4)nm,α=97.70(3)°,β=97.96(3)°,γ=94.33(3)°，V=2.0244(8)nm^3,R1=0.0441andwR2=0.0678,Thecrystalcontainsfourcrystallographicallyindependent[Cu(L-Met)(bipy)(H2O)]^+complexes(Cu1-Cu4),havingadistortedsquare-pyramidalgeometrywiththesamecoordinatedatomsaroundeachcoppercenter.Thebaseplaneisoccupiedbytwonitrogenatomsofonebipy,theaminonitrogenatomandonecarboxylateoxygenatomfromeachindependentL-Metmoiety,andonewateroxygenatanaxialposition.CulandCu3areessentiallyenantiomersofCu2andCu4.Thefourmoleculesarepackedwitheachotherbyintermolecularhydrogen-bondingandaromatic-ringstackinginteractions.

简介：

简介：AIM:Toinvestigatetheeffectsofthefreeradicalscavengerbis(1-hydroxy-2,2,6,6-tetramethyl-4-piperidinyl)decandioate(IAC)inthedextransodiumsulphate(DSS)experimentalmodelofulcerativecolitis.METHODS:ColitiswasinducedinSpragueDawleymaleratsbyadministrationof5%DSSindrinkingwater.IAC(30mg/kg,lipophilicorhydrophilicform)wasadministereddaily(orallyorip)for6duntilsacrifice.Colonicdamagewasassessedbymeansofindirect(DiseaseActivityIndexscore)anddirectmeasures(macroscopicandmicroscopicscores)andmyeloperoxidase(MPO)activity.NeutrophilinfiltrationwithinthetissueandglutathioneS-transferaseactivitywerealsoinvestigated.RESULTS:DSS-inducedcolitisimpairedbodyweightgainandmarkedlyincreasedallinflammatoryparameters.Six-daytreatmentwithlipophilicIACsignificantlyreducedintestinaldamagecausedbyinflammation,inducedadown-regulationinMPOactivity(0.72±0.12and0.45±0.12withlipophilicIACpoandip,respectively,vs1.10±0.27inuntreatedDSScolitisanimals)andminimizedDSS-inducedneutrophilinfiltration,whilehydrophilicIACadministeredorallydidnotameliorateDSS-induceddamage.CONCLUSION:TheseresultssupportthehypothesisthatreactiveoxygenmetabolitescontributetoinflammationandthattheradicalscavengerIAChastherapeuticpotentialininflammatoryboweldisease.

简介：Inthisstudy,weevaluatethevaluesoflatticethermalconductivityκLoftypeIIGeclathrate(Ge34)anddiamondphaseGecrystal(d-Ge)withtheequilibriummoleculardynamics(EMD)methodandtheSlack'sequation.ThekeyparametersoftheSlack'sequationarederivedfromthethermodynamicpropertiesobtainedfromthelatticedynamics(LD)calculations.TheempiricalTersoff'spotentialisusedinbothEMDandLDsimulations.Thethermalconductivitiesofd-Gecalculatedbybothmethodsareinaccordancewiththeexperimentalvalues.ThepredictionsoftheSlack'sequationareconsistentwiththeEMDresultsabove250KforbothGe34andd-Ge.Inatemperaturerangeof200-1000K,theκLvalueofd-GeisaboutseveraltimeslargerthanthatofGe34.

简介：Insitucompatibilizationoflowdensitypolyehylene(LDPE)(30%)andnylon-6(70%)blendsthroughone-stepreactiveextrusionusingt-BuOOHasaninitiatorandlowmolec-ularweightinterfacialagentsascompatibilizerswasstudied.Thecompatibilizercontainedalongchainbydrocarbon,doublebondandtwopolarfunctionalgroupswhichwascapableofreactingwithbothLDPEandnylon-6inthepresenceofinitiatortoformacopolymerattheinterfaceofthetwopolymerphases.Theextrudedblendsexhibitedsignificanten-hancementintheircompatibilitybasedonmorphological,thermalanalysisandmechanicalstudies.Theeffectofthehydrocarbonchainlengthandstructureofthefunctionalgroupofthecompatibilizerwasalsoexamined.Itwasfoundthatblendspreparedbyusingthecompatibilizercontaininglongerhydrocarbonchainandamidegrouphadbettermechanicalproperties.

简介：

简介：为包括在多孔的媒介的分子的散开和分散的一种可压缩的能溶合的排水量问题的联合近似被学习。混合有限元素方法被用于流动方程，和运输一个人被对称的内部惩罚解决不连续的Galerkin方法(SIPG)。避免截止操作员的不方便在[3，21]，与在不同的一些正式就职假设[6]被使用。基于插值设计性质，一个priorihp错误估计被获得。与仅仅处理散开盒子的存在错误分析作比较，当前的工作更复杂、更重要。[从作者抽象]

简介：WeproposeapromisingschemetodecelerateaCWmolecularbeambyusingared-detunedquasi-cwsemi-Gaussianlaserbeam(SGB).WestudythedynamicalprocessofthedecelerationforaCWdeuteratedammonia(ND3)molecularbeambyMonte-Carlosimulationmethod.OurstudyshowsthatwecanobtainaND3molecularbeamwitharelativeaveragekineticenergylossofabout10%andarelativeoutputmolecularnumberofmorethan90%byusingasinglequasi-cwSGBwithapowerof1.5kWandamaximumopticalwelldepthof7.33mK.

简介：尽管有他们在减少害虫人口的积极效果，异国情调的通晓各方面知识经验丰富的人食肉动物有时变得侵略并且在一样的营养的水平贡献土生土长的种类的排水量。尽管实验室实验连接了intraguildpredation(IGP)到这些相互作用，IGP的地证据和quantification是仍然为大多数系统缺乏。异国情调的Harmoniaaxyridis(智慧女神雅典娜)的最近的建立(翘目：Coccinellidae)在意大利，加薪关于瓢虫能在本国的coccinellids上有的有害效果担心。这里，我们估计了，在2个本国的瓢虫的鸡蛋的实验室条件，可接受程度和适用性下面，AdaliabipunctataL。并且Oenopiaconglobata(L.)，为H的猎物项目。axyridis幼虫。然后，我们开发了教材让分子的勇气内容分析由H检测predation。2个瓢虫上并且在蚜虫EucallipterustiliaeL上的axyridis。种类特定的16S教材为3种类被开发，实验室喂审判被进行确定在H的内脏的猎物DNA故障的率。axyridis。而且，评估教材回答，H。axyridis第4中间形态(n=132)系统地在北意大利从菩提树树被收集并且为猎物DNA的存在屏蔽了。百分之73块地和7%收集了H。axyridis分别地为蚜虫和coccinellidDNA是积极的。在蚜虫和A之上的Predation。如果密度依赖者消费被期望，bipunctata比预言低，当时在O之上的predation。conglobata显著地更高。这里，我们在H的凶猛的人口之中提供了IGP的第一条证据。axyridis和土生土长的瓢虫急速移动，发生在意大利。

简介：AbstractBackground:Infectious disease diagnostics often requires sensitive molecular assays that identify at both genus and species levels. For large scale screening, such as malaria screening for elimination, diagnostic assay can be a challenge, as both the throughput and cost of the assay must be considered. The requirement of nucleic acid extraction hampers the throughput of most molecular assays. Co-amplification of multiple species or multiplex identification either can result in missed diagnosis or are too costly for large-scale screening. A genus-and species-specific diagnostic assay with simplified procedure, high sensitivity and throughput is still needed. This study aimed to develop a sensitive and high-throughput approach for large-scale infectious disease screening.Methods:We developed multi-section Capture and Ligation Probe PCR (mCLIP-PCR) for the direct detection of RNA without extraction and reverse transcription. Multiple tailed sandwich hybridization probes were used to bind at genus-and species-specific sections of the target RNA to cooperatively capture the target onto a 96-well plate. After enzymatic ligation of the bound probes, a single-stranded DNA formed at each section with distinct tail sequence at the ends. They were separately PCR-amplified with primers corresponding to tail sequences for genus or species identification. We applied the method to the active screening of Plasmodium infections of 4,580 asymptomatic dried blood spot samples collected in malaria endemic areas and compared the results with standard qPCR using linear regression.Results:With multi-section cooperative capture but separate amplification strategy, we accurately identified genus Plasmodium and species P. falciparum and P. vivax without RNA extraction, with favorable sensitivities among the published reports. In the active screening, our method identified all 53 positive infections including two mixed infections, and two P. vivax infections that were missed by standard qPCR.Conclusions:mCLIP-PCR provides a sensitive and high-throughput approach to large-scale infectious disease screening with low cost and labor, making it a valuable tool for malaria elimination in endemic region.

简介：分子的动态模拟被执行在交流上调查事件精力的效果:从C和H的H电影生长原子流动。我们的模拟证明在低事件精力(1eV)的电影生长被H和C原子的吸附统治。在中等事件精力(10和20eV)，有在表面吸附的H原子的事件H原子的抽象反应变得重要。在高事件精力(30和40eV)，交流:H电影生长是一个二拍子的圆舞过程：一个人是吸附和C原子的浅培植，并且其它是H原子的深培植。[从作者抽象]

简介：Heterogeneousnuclearribonucleoproteins(hnRNPs)arespliceosomalmacromolecularassemblagesandthusactivelyparticipateinpre-mRNAmetabolism.Theyarecomposedofevolutionarilyconservedandtandemlyrepeatedmotifs,wherebothRNA-bindingandprotein-proteinrecognitionoccurtoachievecellularactivities.Byyetunknownmechanisms,theseribonucleoprotein(RNP)particlesaretargetedbyautoantibodiesandhenceplaysignificantroleinavarietyofhumansystemicautoimmunediseases.Thisfeaturemakesthemimportantprognosticmarkersintermsofmolecularepidemiologyandpathogenesisofautoimmunity.SinceRNPdomainisoneofthemostconservedandwidespreadscaffolds,evolutionalysesoftheseRNA-bindingdomainscanprovidefurthercluesondisease-specificepitopeformation.ThestudypresentedhereinrepresentsasequencecomparisonofRNA-recognitionregionsofrecentlyclonedandcharacterizedhumanhnRNPA3withthoseofotherrelevanthnRNPA/B-typeproteins.Theirimplicationsinhumanautoimmunityareparticularlyemphasized.