简介：Combinedmulti-bodydynamicswithstructuraldynamics,anewdiscreteelementwithflexibleconnector,whichisapplicablefor3-Dbeamstructures,isdevelopedinthispaper.Boththegeneralizedelasticcoefficientmatrixoftheflexibleconnectorandthemassmatrixofdiscreteelementmaybeoff-diag-onalinageneralcase.Thezero-lengthrigidelementisintroducedtosimulatethenodeatwhichmultipleel-ementsarejointedtogether.Itmayalsobeeffectivewhentheaxesofadjacentelementsarenotinthesameline.Theexamplesforeigenvaluecalculationshowthatthemodelissuccessful.Itcanbeextendedtothegeometricnonlinearresponseanalysis.

简介：在视口数据检索之后,视口重建效率成为了影响地形数据三维重建和显示速度的主要因素,视口重建与可利用的开发平台资源密切相关.在剖析Direct3D硬件访问方式的基础上,提出了一套完整的DEM快速重建方法.实验结果表明在相同的条件下,基于Direct3D的视口重建具有明显的速度优势.

简介：这篇论文为基于的自我类似论述一个便宜方法由使用高度和反照率地图真实世界的3D表面质地编辑。编辑的基于的2Dtexture接近仅仅做变化到象素颜色或紧张的不同自我类似珍视，这种技术也允许表面几何学和捕获的3D表面质地的反射到用原版不同于那些的照明条件和视场角是ited和relit。在一个给定的地点的单个编辑操作影响所有类似的区域并且在在不同条件下面显示的样品的所有图象上生产变化。后来，表面高度和反照率地图能被用来描述海床地形学和地质的特征，它在许多海洋的进程起重要作用，建议方法能有效地关于海洋的现象的可视化和模拟在应用程序被采用。

简介：Atime-domainmethodisappliedtosimulatenonlinearwavediffractionaroundasurfacepiercing3-Darbitrarybody.ThemethodinvolvestheapplicationofTaylorseriesexpansionsandtheuseofperturbationproceduretoestablishthecorrespondingboundaryvalueproblemswithrespecttoatime-independentfluiddomain.AboundaryelementmethodbasedonB-splineexpansionisusedtocalculatethewavefieldateachtimestep,andthefreesurfaceboundaryconditionissatisfiedtothesecondorderofwavesteepnessbyanumericalintegrationintime.Anartificialdampinglayerisadoptedonthefreesurfacefortheremovalofwavereflectionfromtheouterboundary.Asanillustration,themethodisusedtocomputethesecond-orderwaveforcesandrun-uponasurface-piercingcircularcylinder.Thepresentmethodisfoundtobeaccurate,computationallyefficient,andnumericallystable.

简介：柱体的垂直摆动与不同直径比率Dd/Dc和厚度比率td/Dc被3D学习(三维)在这份报纸开发的数字模型，并且通过2D与结果相比获得了(二维)数字模型。高顺序的迎风的计划被使用稳定计算，并且集中被多格子方法加速。2D和3D模拟结果之间的差别的质、量的分析与一个依附底部的磁盘在流动地特征和垂直地震荡的柱体的水动力学系数上揭示3D效果。液体领域上的3D效果主要在三个流旋涡的模式的意义被反映：x相对在2D模拟产生的旋涡在锋利的边附近在流动地上有更大的效果。在沿着轴的取向的片，沿着光线的轴的y比沿着环绕的方向的x，更在速度上显示光线的效果在磁盘的锋利的边附近比环绕的效果读了。旋转相互作用在水平飞机的液体的z在举运动期间是不足道的。基于2D和3D模拟结果，分开增加的集体系数和抑制比率的增加的政体的转弯的点被识别。直径比率Dd/Dc和厚度比率td/Dc详细被讨论。

简介：Astochastictwo-dimensionalFornasini-Marchesini'sModelⅡ(2-DFMMⅡ)withmultiplicativenoiseisgiven,andafilteringalgorithmforthismodel,whichisoptimalinthesenseoflinearminimum-variance,isdeveloped.Thestochastic2-DFMMⅡwithmultiplicativenoisecanbereducedtoa1-Dmodel,andtheproposedoptimalfilteringalgorithmforthestochastic2-DFMMⅡwithmultiplicativenoiseisobtainedbyusingthestateestimationtheoryof1-Dsystems.Anexampleisgiventoillustratethevalidityofthisalgorithm.

简介：Inthisstudy,thecombinedactionsofwavesandtidalcurrentsinestuarineandcoastalareasareconsideredanda2Dmathematicalmodelforsedimenttransportbywavesandtidalcurrentshasbeenestablishedinorthogonalcurvilinearcoordinates.Non-equilibriumtransportequationsofsuspendedloadandbedloadareusedinthemodel.Theconceptofbackgroundconcentrationisintroduced,andtheformulaofsedimenttransportcapacityoftidalcurrentsfortheOujiangRiverestuaryisobtained.TheDouGuorenformulaisemployedforthesedimenttransportcapacityofwaves.SedimenttransportcapacityintheformofmudandtheintensityofbacksiltingarecalculatedbyuseofLuoZaosen'sformula.Thecalculatedtidalstagesareingoodagreementwiththefielddata,andthecalculatedvelocitiesandflowdirectionsof46verticallinesfor8crosssectionsarealsoingoodagreementwiththemeasureddata.Onsuchabasis,simulationsofbacksiltingafterexcavationofthewaterwaywithasandbarundercomplicatedboundaryconditionsinthenavigationchannelinducedbysuspendedload,bedloadandmudbywavesandtidalcurrentsarediscussed.

简介：羟丙基壳聚糖（惠普壳聚糖）已被证明具有在广泛领域的应用前景由于其良好的生物相容性，生物降解性和生物活性，特别是在生物医学和制药领域。然而，它的药代动力学和生物降解性能，这是至关重要的，其临床应用尚不清楚。为了进一步开发和应用奠定基础，我们在这里进行荧光强度分析和GPC测定异硫氰酸荧光素标记的惠普壳聚糖的药代动力学模式（FITC惠普壳聚糖）及其生物降解性研究结果表明，在剂量为每只大鼠腹腔注射后，FITC标记的壳聚糖能迅速吸收，惠普分布于肝，肾和脾的血。结果表明，壳聚糖能有效利用FITC惠普，和惠普的FITC标记的壳聚糖88.47%可经尿排泄在11天内与分子量小于10kDa。此外，我们的数据表明，有一个明显的降解过程发生在肝脏（＜1024h）。综上所述，壳聚糖具有良好的生物利用度和生物降解性，惠普，表明羟丙基壳聚糖潜在的应用在药物缓释材料，组织工程和生物医学领域。

简介：Cytarabine(1-β-D-arabinofuranosylcytosine,Ara-C),isolatedfromaCaribbeanspongespeciesTethyacrypta,isthefirstantitumordrugfromamarineresource.In1980,theUSFoodandDrugAdministrationapprovedthisdrugforthetreatmentofdifferenttypesofleukemia.Thisdrughasashortplasmahalf-life,lowstability,limitedbioavailability,andseveresideeffects.Toimprovestabilityandbioavailability,wesynthesizedninenovelderivativesbyblockingthecytarabinemetabolicsitesandimprovinglipophilicity.ThecLogPvaluesofthenewlysynthesizedcompoundswerecalculated.Allthesynthesizedcompoundsweremorelipophilicthancytarabine,resultinginmembranepermeabilityandbioavailabilityimprovement.TheantitumoractivitiesagainstleukemiacelllineHL-60wereevaluatedbyusingtheMTTassay.ThebioassayresultsrevealedthattheIC50valuesofcompounds5,8and9were0.080,0.090and0.057μmolL-1,respectively,whichwassimilarwiththatofcytarabine(0.056μmolL-1).Incomparison,compound4withaphosphategroupatO5’wasinactive.Becausephosphoesterbondsareeasilyhydrolyzedbyalkalinephosphataseandarecommonlyusedinproducingprodrugstrategies,compound4mightalsobemetabolizedinvivoandgeneratecompound3orevencytarabinethroughamulti-stepreaction.Thus,compound4mightbeapromisingcompoundtobedevelopedasaprodrug.

简介：Inthispaper,thefocusedwavegroupswithdifferentparametersandtheiractionsonaverticalcylinderareexperimentallystudied.Theharmonicwavecharacteristicsofthefocusingwavesareanalyzedbytheadditionandsubtractionofthecrestandtroughfocusingwaves.Theanalyzedresultsshowthathigherorderharmonicscanbegeneratedbecauseoftheinteractionofcomponentwaves.Nonlinearityincreaseswiththeinputtedwaveamplitudeandthefrequencywidthincrement.Further,thewaverun-uparoundtheverticalcircularcylinderisexperimentallystudied.Itincreaseswiththewavesteepnessandtherelativecylinderdiameterincrease.However,thevariationsofwaverun-uparoundthecircularcylinderaredifferent.Theresearchesprovideareferenceforfurthernumericalstudies.

简介：HC-90D型地磁日变站系统的主体是高精度光泵磁力仪.比较详细地介绍了HC-90D型地磁日变站工作原理,阐述了架设日变站的方法和注意事项.

简介：通常一个维(1-D)实验咸度侵入模型被限制到自然冲积河口。然而，这研究试图调查它的能力在马来西亚为Terengganu河的一个掩盖的冲积河口建模。在河的嘴的构造防波提从公海的直接影响掩盖河口。沿着河口的咸度密度被收集在期间湿并且为在构造防波提前后的情形的旱季。而且，淡水分泌物，潮汐的举起和深测术数据也作为模型输入被测量。一好合适在模仿并且观察的变量之间被表明，也就是为两种情形的咸度分发和侵入长度。因此，结果显示出那1-D实验咸度模型能在Terengganu河口为掩盖的河口状况被利用，但是与一个起始的点的一颗适当决心。而且，在学习区域的咸度侵入大部分依赖于淡水分泌物而非潮汐的举起变化，这被观察。在学习区域的咸度侵入长度的规模与the-1/2力量的河分泌物成正比。实验咸度模型和分泌物驱动的1-D的二根线基于适合很好到对方的方程，这被显得，与150的平均预言的最小的淡水分泌物，m3/s将被要求在水吸入车站附近在高水位线呆滞(HWS)期间维持可接受的咸度层次，它从河嘴在10.63km被定位。

简介：Astheexplorationandexploitationofoilandgasproliferatethroughoutdeepwaterarea,therequirementsonthereliabilityofdynamicpositioningsystembecomeincreasinglystringent.Thecontrolobjectiveensuringsafetyoperationatdeepwaterwillnotbemetbyasinglecontrollerfordynamicpositioning.Inordertoincreasetheavailabilityandreliabilityofdynamicpositioningcontrolsystem,thetripleredundancyhardwareandsoftwarecontrolarchitecturesweredesignedanddevelopedaccordingtothesafespecificationsofDP-3classificationnotationfordynamicallypositionedshipsandrigs.Thehardwareredundantconfigurationtakestheformoftriple-redundanthotstandbyconfigurationincludingthreeidenticaloperatorstationsandthreereal-timecontrolcomputerswhichconnecteachotherthroughdualnetworks.Thefunctionofmotioncontrolandredundancymanagementofcontrolcomputerswereimplementedbysoftwareonthereal-timeoperatingsystemVxWorks.Thesoftwarerealizationoftaskloosesynchronization,majorityvotingandfaultdetectionwerepresentedindetails.Ahierarchicalsoftwarearchitecturewasplanedduringthedevelopmentofsoftware,consistingofapplicationlayer,real-timelayerandphysicallayer.ThebehavioroftheDP-3dynamicpositioningcontrolsystemwasmodeledbyaMarkovmodeltoanalyzeitsreliability.Theeffectsofvariationinparametersonthereliabilitymeasureswereinvestigated.Thetimedomaindynamicsimulationwascarriedoutonadeepwaterdrillingrigtoprovethefeasibilityoftheproposedcontrolarchitecture.

简介：降雨在海表面上有二重要效果，包括咸度减少并且表面变得更不平，它在L乐队海表面放射率上有进一步的影响。用宝瓶座和TRMM3B42配对数据集的调查显示检索的海表面咸度(SSS)被现在的宝瓶座算法与数字模型产量相比低估，特别在高雨率的情况下。例如，当雨率是25mm/h时，在观察卫星的SSS之间的偏爱和数字模型SSS是约2。偏爱能被财务为导致雨的粗糙消除，它被产生雨的戒指波浪光谱通常建模。雨光谱将是进为雨影响的海表面放射率的模拟的小斜坡近似(SSA)模型的输入。与理论模型一起的比较显示对更合适的雨spectrumis的实验模型在模拟被使用。进一步，雨光谱的系数被与2year宝瓶座和TRMM配对数据集的观察适合模拟修改。计算证实海表面放射率以风速度和雨率增加。雨率导致的增加在低雨率和低风速度的情况中是快速的。最后，包括雨光谱的海表面放射率的一个修改模型被在2014年5月使用配对数据集建议并且验证。与观察相比，导致雨的海表面放射率的偏爱近似由修改modelis模仿了1e4,和RMSE比1e3稍微大。与使用更多的配对数据，在模型之间的thebias检索了海表面咸度和observationsmay进一步被改正，并且RMSE可以在低雨率和低风速度的情况中被归结为不到1。

简介：Outerdensefibersarespecificsubcellularcomponentsofmammalspermflagella,apartfromtheaxonemeanditsassociatedproteins.Asoneoftheouterdensefiberscomponents,ODF3isessentialfortheformationofmammalspermflagella.Inthepresentstudy,weisolatedtheOdf3gene,1033bpinlength,fromZhikongscallopChlamysfarreri,whichwasnamedasCf-Odf3.ItwaspredictedthatCf-Odf3encodesaproteinof240aminoacidswhichcontains5discontinuousPro-Gly-Prorepeats.TheCf-Odf3transcriptsweredetectedinbothtestisandovaryofC.farreriatvariousstagesthroughqRT-PCR.TheCf-ODF3proteinsynthesizedbyprokaryoteswaspurifiedusingNi2+-NTAaffinitychromatographyandusedtoproduceitspolyclonalantibody.TheantibodyproductwaseffectiveandspecificaswasexaminedbyWesternblotting.TheCf-Odf3transcriptandCf-ODF3proteinwerelocatedinintragonadalsomaticcells(ISCs),spermatogoniaandspermatocytesofC.farreritestes,whilenotranscriptionofCf-Odf3andsynthesisofCf-ODF3weredetectedinspermatidsandspermatozoa.ThelocationofOdf3expressionisdifferentfromthatofvertebrateswhereODF3issynthesizedspecificallyinspermatidsandspermflagella.Moreover,theCf-Odf3expressionwasalsodetectedinISCs,oogoniaandoocytesofthescallopovaries.OurfindingrevealedadifferentcharacteristicofOdf3expressionbetweenscallopandvertebrates,whichimpliedthatCf-Odf3playedaroleinthegametogenesisofC.farreri.

简介：EvolutionaryevidencesuggeststhatSox3,amemberofthehigh-mobility-group(HMG)familyoftranscriptionfactors,isanancestralprecursorofSryandisinvolvedinsexdeterminationsimilartoSry.However,thereislimitedinformationregardingtheSOX3geneoftheblackrockfish(Sebastesschlegeli).Inthisstudy,wefirstisolatedSOX3genefromthegonadsofS.schlegelibyhomologycloning.Thefull-lengthofS.schlegeliSOX3(SsSOX3)cDNAwas1386bp,comprisinga906-bpopenreadingframe,whichencodesapeptideshowing93.6%and93.9%homologywiththeSox3proteinsofEpinepheluscoioidesandOryziaslatipe,respectively.ComparisonofthecDNAsequenceoftheSsSOX3genewiththecorrespondinggenomicDNAfragmentrevealedthattheSsSOX3geneconsistsofasingleexon.PhylogeneticanalysisdemonstratedtheevolutionaryrelationshipofSsSOX3withotherknownSOXB1genesinfishandtetrapods.ThepromoterregioncontainsbindingsitesofseveraltranscriptionalfactorsthatmightparticipateintheregulationofSsSOX3expression.Quantitativereal-timePCRanalysisindicatedthatSsSOX3wasexpressedinalltheinvestigatedlarvaldevelopmentalstagesfrom1to35daysafterbirthandthelevelofexpressiongraduallydecreasedasthedevelopmentproceeded.SsSOX3exhibitedsexuallydimorphicexpressioninadultgonads,withhighexpressionintheovarybutlowexpressioninthetestis.InsituhybridizationrevealedthatSsSOX3wasstronglyexpressedinoocytesandfollicularcellsofovariesbutslightlyexpressedingermcellsoftesticulartissues.Therefore,thisstudysuggeststhatSsSOX3playsanimportantroleinoogenesisandovarydifferentiationinS.schlegeli.

简介：我们曾报道了短梗霉菌产生的高纤维素酶产量98。在这项研究中，羧甲基纤维素酶（CMCase）在培养的细胞。短梗霉98的纯化至均一，与酶的最大产量为4.51U（mg蛋白）-1。SDS-PAGE分析表明，纯化的酶的分子量为67.0kda。具有相当的敏感性为40℃纯化的酶的最适温度，比从其他真菌的cmcases低得多。该酶的最佳pH值为5.6，和活动的个人资料被稳定在一个范围内的酸度（pH5,0-6.0）。这种酶被激活Na＋，Mg2＋，Ca2＋，K＋，Fe2＋和Cu2＋，然而，它是由Fe3＋，Ba2＋，Zn2＋，Mn2＋和银离子抑制。公里和纯化的酶的Vmax值4.7mgml-10.57pmolL-1min-1（mg蛋白）1，分别。只有大小不同的低聚糖，羧甲基纤维素（CMC）释放与纯化的酶水解后。该基因编码的酶是A.霉98个克隆，其中包含一个开放阅读框（eu978473）意义。推导的蛋白质含有酶超家族的保守结构域（糖基水解酶家族5）。的N-末端氨基酸序列的纯化的酶是m-a-p-h-a-e-p-q-s-q-t-t-e-q-t-s-s-g-q-f，这与从克隆的基因推导一致。这表明，纯化的酶是由克隆纤维素酶基因在酵母编码。

简介：AbundantevidencesofhighersealevelsfromJiangsuandFujiancoastshaveprovedamarinetransgressioneventduring30–40kaBP,suggestingthattherewasastagewithhighsealevelandawarmclimatewhenicesheetsshrankintheNorthernHemisphere.Thedurationof30–40kaBPspannedaperiodinthelateMarineIsotopeStage3(MIS3)andwasinnatureaninterstadialepochduringtheLastGlacialperiodoftheQuaternary.Differentfromtheglacialperiodwithacoldclimate,thismarinetransgressionconsideredasapenultimatehighersealevelduringtheQuaternaryremainsapuzzlethatwhytheevidenceiscontrarytotheQuaternaryglacialtheory.Itisimportanttounderstandsealevelrisefortheseareassensitivelyrespondingtotheglobalchangesinthefuture.Torecognizethekeyissuesonsealevelchanges,theeustaticsealevel(H_S)wasdefinedastheglaciation-climateforcedsealevels,andtherelativesealevelchange(H_R)wasdefinedasthatasealevelrecordwaspreservedinsedimentthatexperiencedmultiplesecondaryactionsoflandandseaeffects.Onthebasisasdefinedabove,weconstructedmulti-levelmodelsofclimate-drivenglacio-eustaticchangesandland-seasystems.Byintegratingdatasetsfromeightboreholecoresandprescribingtheboundaryconditions,wesimulatedthechangesofHSandHRintheEastChinaSeaandsouthernYellowSeaareasinthelateMIS3.Themarinetransgressionstratafromtheboreholecoredatawasidentifiedatca.30mbelowpresentsealevelasaresultofthecollectiveinfluenceoficemeltingwater,neotectonicsubsidence,sedimentcompactionandterrestrialsedimentfillingsinceca.35kaago,whereasthesimulatedrelativesea-levelsturnedouttobe–26.3––29.9ma.s.l.Thesmallerrorinvolvedinthesimulationresultsof±(2.5–4.5)mdemonstratedthecredibilityoftheresults.OurresultsindicatedthatsealevelchangeinthelateMIS3wasdominatedbyglacialeffects,inwhichtheeustaticsea-levelwasbetween–19.2�

简介：FattyacidcompositionsoftwostrainsofmarinediatomsNitzschiaclosterium(MACCB222)andChaetocerosgracilis(MACCB13)havebeenexamined.ThemicroalgaehavebeengrownatdifferentinitialNaNO3concentrations(75,375,750,1125,1500,1875mg/L)andharvestedatthelateexponentialphase.TheresultsbyonefactoranalysisofvarianceshowthattheNaNO3concentrationshavenosignificantinfluenceontherelativegrowthrateoftwostrainsofmarinediatoms;theinfluenceofNaNO3concentrationsonthefattyacidsdiffersfromspeciestospecies.Themajorfattyacidsoftwodiatomstrainsare14:0,16:0,16:ln-7and20:5n-3(EPA),B13alsohashighpercentageof20:4n-6(6.0～9.1%).

简介：有不同规模的很多海底的山崩在Liwan3-1气体地的海底的管道线路的峡谷区域被识别了。仍然有许多机会，海底的斜坡失败将发生，并且下列集体运动将介绍大风险给海底的管道。鉴于这，一个数字预言方法基于Eulerian-Eulerian二阶段的流动模型被介绍模仿潜在的海底的山崩的集体运动。滑动土壤和周围的水被Herschel-Bulkley流变学模型和牛顿的液体模型分别地模仿。骚乱用k-模型被模仿。与两个相比实验数据并且是结果，二阶段的流动模型显示出好精确性，并且它的结果是更多关门到实际状况；在土壤和周围的水之间的动态联合能有效地被模仿，hydroplaning和头分开的现象能被获得。最后，潜在的海底的山崩的土壤运动作为一个例子被模仿，根据在峡谷区域的地震侧面。结果证明hydroplaning发生在运动过程期间。二阶段的流动模型计算的刺杀距离是877m，它是27.1%比是大结果。然而，土壤的山峰前面速度是相对的小，与8.32m/s的最大的价值。是有一个简单、快速的过程的节目能被用于初步的评估，当二阶段的流动模型为一个精确评价是更适当的时。