简介:客户端的私有端点地址被NAT设备,2)服务器提供客户端端点地址消息,该数据报使得C2网络的NAT设备允许后续的以C1端点地址为源地址的UDP数据报进入其内部网络
简介:Objective:Toinvestigatethepost-transcriptionalregulationofp21WAF1/CIP1byp53.Methods:TheMDA-MB-468cellshaveendogenousmutantp53andtheMCF7cellslineshavewtp53.Recombinantp53expressionandp21WAF1/CIP1inductionweredetectedbyWesternblotanalysis.Northernblotanalysiswascarriedouttoexaminewhetherchangesinp21WAF1/CIP1proteinlevelsinMCF7cellstreatedwithAdCMVp53arereflectedatthemRNAlevel.FlowcytometricanalysisofMCF7cellsfollowingoverexpressionofrecombination.Results:Theratioofp53:p21WAF1/CIP1wasbelow1attheearlystagesofAdCMVp53infection,butincreasedto1.6byday3andto9.7byday5post-infection.Asexpected,p21WAF1/CIP1expressionwasnotdetectableinMDA-MB-468cellsdespitethepresenceofhighlevelsofmutantp53protein.TheG1/SratiosinuntreatedcontrolsandAdCMVβgalinfectedMCF7cellswere1.10and1.35,respectively.ByNorthernblotanalyzingthep21WAF1/CIP1:GAPDHratiosatdifferenttimepointsagainsttheratioattimepoint0,amaximum3-foldinductionofp21WAF1/CIP1mRNAexpressionrelativetountreatedcontrolwasobservedonday1post-infection.TheflowcytometricanalysisindicatedthatMCF7cellsinfectedwithAdCMVp53undergoG1arrestatbothtimepointsstudied,withG1/Sratiosrangingfrom5.54atday1to5.65atday7.TheG1/SratiosinuntreatedcontrolsandAdCMVβgalinfectedMCF7cellswere1.10and1.35,respectively.Conclusion:Thisstudydemonstratedthatp53couldregulatep21WAF1/CIP1geneexpressionatboththetranscriptionalandpost-transcriptionallevelsinMCF7cells.Thelattermechanismmaybeinvolvedinorberesponsiblefor,theinductionofcellcyclearrestbytranscription-defectivemutantsofp53.
简介:摘要随着网络技术不断提高与电子商务的迅猛发展,C/S模式将不能满足需要,一种新的网络应用模式P2P能促进电子商务的进一步发展,P2P电子商务将成为一种趋势。本文对P2P电子商务的必然性和可行性进行了分析探讨。
简介:Inthispaper,theLp-convergenceofGrünwaldinterpolationGn(f,x)basedonthezerosofJacobipolynomialsJ(α,β)n(x)(-1<α,β<1)isconsidered.Lp-convergence(0<p<2)ofGrünwaldinterpolationGn(f,x)isprovedforp·Max(α,β)<1.Moreover,Lp-convergence(p>0)ofGn(f,x)isobtainedfor-1<α,β≤0.Therefore,theresultsof[1]and[3-5]areimproved.
简介:Inthispaper,weintroduceaconditionweakerthantheLpdifferentiability,whichwecallCpcondition.Weprovethatifafunctionsatisfiesthisconditionatapoint,thenthereexiststhebestlocalapproximationatthatpoint.WealsogiveanecessaryandsufficientconditionforthatafunctionbeLpdifferentiable.Inaddition,westudytheconvexityofthesetofclusterpointsofthenetofbestappoximationsoff,{Pe(f)}ase→0.