简介：Ginkgolicacids(GAs),primarilyfoundintheleaves,nuts,andtestaofginkgobiloba,havebeenidentifiedwithsuspectedallergenic,genotoxicandcytotoxicproperties.However,littleinformationisavailableaboutGAstoxicityinkidneysandtheunderlyingmechanismhasnotbeenthoroughlyelucidatedsofar.InsteadofGAsextract,therenalcytotoxicityofGA(15:1),whichwasisolatedfromthetestaofGinkgobiloba,wasassessedinvitrobyusingMDCKcells.TheactionofGA(15:1)oncellviabilitywasevaluatedbytheMTTandneutralreduptakeassays.Comparedwiththecontrol,thecytotoxicityofGA(15:1)onMDCKcellsdisplayedatime-anddose-dependentmanner,suggestingthecellsmitochondriaandlysosomesweredamaged.ItwasconfirmedthatGA(15:1)resultedinthelossofcellsmitochondrialtrans-membranepotential(ΔΨm).Inpropidiumiodide(PI)staininganalysis,GA(15:1)inducedcellcyclearrestattheG0/G1andG2/Mphases,influencingontheDNAsynthesisandcellmitosis.CharacteristicsofnecroticcelldeathwereobservedinMDCKcellsattheexperimentalconditions,asaresultofDNAagarosegelelectrophoresisandmorphologicalobservationofMDCKcells.Inconclusion,thesefindingsmightprovideusefulinformationforabetterunderstandingoftheGA(15:1)inducedrenaltoxicity.

简介：根据中药方剂研究特点,按正交设计原理及F值与t值的特定关系,提出“正交t值法”。数理上与正交设计F值法等价,原理清晰,计算简便,统计结论完全一致。实验分三步进行(主药分析,辅药交互分析,剂量选择),并改进了正交表形式,便于分析两药间的协同或拮抗作用,适用于中药较大方剂的研究。主要公式为:残差标准误自由度显著性检验二水平时预期药效二水平时

简介：Consideringthegreatpotentialofnaturalproductsasanticanceragents,thepresentstudywasdesignedtoexplorethemolecularmechanismsresponsibleforanticanceractivitiesofMesuaferreastembarkextractagainsthumancolorectalcarcinoma.BasedonMTTassayresults,bioactivesub-fraction(SF-3)wasselectedforfurtherstudiesusingHCT116cells.Repeatedcolumnchromatographyresultedinisolationoflessactiveα-amyrinfromSF-3,whichwasidentifiedandcharacterizedbyGC-MSandHPLCmethods.α-amyrinandbetulinicacidcontentsofSF-3weremeasuredbyHPLCmethods.Fluorescentassaysrevealedcharacteristicapoptoticfeatures,includingcellshrinkage,nuclearcondensation,andmarkeddecreaseinmitochondrialmembranepotentialinSF-3treatedcells.Inaddition,increasedlevelsofcaspases-9and-3/7levelswerealsoobservedinSF-3treatedcells.SF-3showedpromisingantimetastaticpropertiesinmultipleinvitroassays.Multi-pathwayanalysisrevealedsignificantdown-regulationofWNT,HIF-1α,andEGFRwithsimultaneousup-regulationofp53,Myc/Max,andTGF-βsignallingpathwaysinSF-3treatedcells.Inaddition,promisinggrowthinhibitoryeffectswereobservedinSF-3treatedHCT116tumourspheroids,whichgiveahintaboutinvivoantitumorefficacyofSF-3phytoconstituents.Inconclusion,theseresultsdemonstratedthatanticancereffectsofSF-3towardscoloncancerarethroughmodulationofmultiplemolecularpathways.

简介：介绍Cystone是为各种各样的尿混乱在印度使用的同意的Ayurvedicpolyherbal专卖药品，包括urolithiasis。试图在urolithiasis把Cystone的保护的效果与导致hyperoxaluria的氧化应力和钙盐水晶免职作比较。方法乙烯乙二醇(例如)(0.75%，V/V)在喝，水被给老鼠28天与Cystone(500和750mg/kg身体重量)的同时的处理导致urolithiasis，并且urolithiasis的各种各样的尿风险因素和抗氧化剂标记被估计。例如结果处理导致增加的尿体积和降低的尿pH与盐的增加的尿排泄一起，在未经治疗的动物的钙和磷酸盐。这些变化在未经治疗的老鼠的肾引起了广泛的钙盐水晶免职，增加的类脂化合物peroxidation和抗氧化剂酶(草皮，过氧化氢酶和GPx)的减少的活动。Cystone阻止了这些hyperoxaluric表明并且在两剂量在对待的老鼠禁止了钙盐水晶免职。结论Cystone治疗由改进肾的织物抗氧化剂地位和多尿对导致hyperoxaluria的氧化应力和钙盐水晶免职提供保护。

简介：Marsdeniaetenacissimaeextract(MTE),commonlyknownasXiao-Ai-PinginChina,isatraditionalChineseherbmedicinecapableofinhibitingproliferationandmetastasisandboostingapoptosisinvariouscancercells.However,littleisknownaboutthecontributionofMTEtowardstumorangiogenesisandtheunderlyingmechanism.ThepresentstudyaimedtoevaluatetheeffectsofMTEontheproliferationandapoptosisofhumanumbilicalveinendothelialcells(HUVECs)andthemolecularism.3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethoxyphenyl)-2-(4-sulfopheny)-2H-tetrazolium,innersalt(MTS)andPI-stainedflowcytometryassaysrevealedthatMTEdose-dependentlyreducedtheproliferationofHUVECsbyarrestingcellcycleatSphase(P<0.05).AnnexinV-FITC/PI-stainedflowcytometryconfirmedthatMTE(160μL·mL-1)enhancedtheapoptosisofHUVECssignificantly(P<0.001).Real-timequantitativeRT-PCRandWesternblotanalysesshowedanincreaseinBaxexpressionandasharplydeclineinBcl-2expression;caspase-3wasactivatedsimultaneouslyinadose-dependentmanner(P<0.05).Furtherstudyobservedthedose-dependentdown-regulationofvascularendothelialgrowthfactor(VEGF)receptor-2(VEGFR-2),P2Y6receptor(P2Y6R),andchemokine(C-Cmotif)ligand2(CCL-2),alongwiththeactivationofPKCδandup-regulationofp53inadose-dependentmannerinMTE-treatedselectedcells(P<0.05).Collectively,theresultsfromthepresentstudysuggestedthatMTEsuppressedtheproliferationbyattenuatingCCL-2-mediatedVEGF/VEGFR2interactionsandpromotedtheapoptosisthroughPKCδ-inducedp53-dependentmitochondrialpathwayinHUVECs,supportingthatMTEmaybedevelopedasapotentanti-cancermedicine.

简介：目的：探讨羧甲基茯苓多糖（CMP）对小鼠T淋巴细胞分泌IFN-γ、IL-10的影响。方法：CMP溶液给小鼠腹腔注射，10天后取外周血分离T细胞，体外将不同浓度的CMP与T细胞共培养，MTT法观察其对T细胞的毒性作用，ELISA法检测IFN-γ、IL-10的含量。结果：CMP在0～500μg／ml对小鼠T细胞无明显毒性作用，CMP能显著提高IFN-γ水平，降低IL-10含量。结论：CMP具有较好的调节Th1／Th2细胞因子分泌作用。

简介：目的：利用人脐静脉内皮细胞体外培养模型，探讨肾上腺素刺激状态下海带多糖L01对内皮细胞的保护作用，以及其对其分泌的组织型纤溶酶原激活物（t-PA）的影响，从内皮细胞抗血栓功能方面探讨海带多糖L01对心血管系统的保护作用。方法：体外培养人脐静脉内皮细胞系ECV-304，用肾上腺素刺激24h、48h、72h采样，ELISA法测定HUVECs培养上清液中的t-PA含量，逆转录多聚酶链反应（RT-PCR）检测HUVEC细胞内t-PAmRNA的表达，其扩增产物经电泳后密度扫描及半定量分析。结果：肾上腺素模型组HUVECs培养液中t-PA（24h、48h）含量明显增加，；单给海带多糖对HUVECst-PA分泌无影响。海带多糖L01在肾上腺素刺激下给药24h，48h后t-PA抗原分泌明显降低（P〈0．01），且具有一定的量效关系，但HUVECst-PAmRNA表达各组均无明显差异。结论：肾上腺素可刺激内皮细胞分泌t-PA抗原，海带多糖L01对血管内皮具有一定的保护作用，减少肾上腺素刺激下HUVECs分泌t-PA，而对t-PAmRNA表达无明显影响。