简介：AIMToexpressandpurifyLipoprotein-associatedphospholipaseA2(Lp-PLA2),andtoestablishascreeningmodelforLp-PLA2inhibitorsthroughtherecombinantLp-PLA2.METHODSThefull-lengthgeneofLp-PLA2wasclonedfromthedifferentiatedTHP-1cellsbyRT-PCRandPCR.TheLp-PLA2genewassubclonedintothePichiaexpressionvectorpPIC9andintroducedasequenceencodingaC-terminalstretchofsixhistidineresiduesatthesametime.TherecombinantplasmidwastransformedintoPichiapastorisGS115byspheroplastingandthegenewasthenintegratedintotheGS115genome.Lp-PLA2wasexpressedintheyeaststrainGS115byinducingwith0.5%methanol.
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