简介：ＴＨＥＥＦＦＥＣＴＯＦＯＸＹＧＥＮＡＰＰＬＩＥＤＴＨＲＯＵＧＨＤＲＩＤＳＨＩＰＣＯＮＳＴＡＮＴＨＥＡＤＶＥＳＳＥＬＴＯＤＬＡＬＹＳＡＴＥＯＮＤＩＡＬＹＳＩＳＨＹＰＯＸＥＭＩＡＺｈｅｎｇｕｏＭｉ，ＹｕｓｈｅｎｇＢａｏ，ＣｈｕｎＬｉｕ，ＸｉＣａｏ，Ｊｉａ...

简介：Tomakecleartheprecisehemodynamicmechanismunderlyingtheanti-atherogenesisbenefitofenhancedexternalcouterpulsation(EECP)treatment,andtoinvestigatetheproperroleofsomeimportanthemodynamicfactorsduringtheatheroscleroticprogress,acomprehensivestudycombininglong-termanimalexperimentandnumericalsolvingwasconductedinthispaper.AnexperimentallyinducedhypercholesterolemicporcinemodelwasdevelopedandthechronicEECPinterventionwassubjected.Basichemodynamicmeasurementwasperformedinvivo,aswellasthearterialendothelialsampleswereextractedforphysiologicalexamination.Meanwhile,anumericalmodelwasintroducedtosolvethecomplexhemodynamicfactorssuchasWSSandOSI.TheresultsshowthatEECPtreatmentresultedinsignificantincreaseoftheinstantlevelsofarterialWSS,bloodpressure,andOSI.DuringEECPtreatment,theinstantOSIlevelofthecommoncarotidarteriesovercardiaccyclesraisedtoameanvalueof8.58×10-2±2.13×10-2.Meanwhile,thechronicinterventionofEECPtreatmentsignificantlyreducedtheatheroscleroticlesionsinabdominalaortasandtheendothelialcellularadherence.ThepresentstudysuggeststhattheuniquebloodflowpatterninducedbyEECPtreatmentandtheaugmentationofWSSlevelincardiaccyclesmaybethemostimportanthemodynamicmechanismthatcontributetoitsanti-atherogenesiseffect.Andasoneoftheindicesthatcausegreatconcernincurrenthemodynamicstudy,OSImaynotplayakeyroleduringtheinitiationofatherosclerosis.更多还原

简介：Toinvestigatethehistologicalchangeandtheexpressionsofepidermalgrowthfactor(EGF)andepidermalgrowthfactorreceptor(EGFR)inatrophicgastricmucosainratssoastoappraisetheeffectoftheseregulatorsontheformationofatrophicgastritis,tostudytheexpressionsofEGFandEGFRinratswithchronicatrophicgastritis(CAG)aftertheirradiationofHe-Nelaser,andtoinvestigatetherelationsofHe-Nelaserandprecancerouslesionorapoptosis.Theratsweredividedintonormalgroup,modelgroupandlasergroup.ThemodelsofCAGratswereestablishedwithenemawhichwasthemixedliquorconsistedofsodiumsalicylateandalcohol,combinedwithirregularfastingandcompulsivesportingaspathogenicfactors.He-Nelaser(3.36J/cm2)wasusedtoirradiateCAGrats,onceadayfor7min,20daysasacourseoftreatment;theexpressionsofEGFandEGFRweredetectedwithimmunohistochemicalmethod.Duringtheprocessofantralatrophy,theexpressionsofEGFandEGFRincreased,theywerehigherinmodelgroupthanthoseinnormalgroup(P<0.05).AftertheirradiationofHe-Nelaser,theexpressionsofEGFandEGFRwereobviouslylowerthanthoseinmodelgroup(P<0.05).Wedrawtheconclusionasfollows:gastricmucosaofmodelratisinahyper-proliferationstatus,withhighproteinexpressionsofapoptosissuppressorEGFandEGFR.CAGhassomecorrelationwiththeimbalancebetweencellproliferationandapoptosis.He-Nelaser(3.36J/cm2)canreducetheexpressionsofEGFandEGFR,whichisaneffectivephysiostimulatortostimulatethegastricmucosaofrat.TheappropriatesecretionsofEGFandEGFRarepropitioustotherepairandregenerationforthegastricmucosatissues,thuspreventingCAGtocanceration.

简介：客观：击倒链球菌mutans(S.mutans)的全部勒克司基因经由相应再结合和构造的UA159紧张S的删除勒克司的变异的紧张。Mutans。学习S的酸抵抗之间的差别。MutansIngbrittC国际标准紧张和勒克司变异的紧张的酸抵抗。方法：二DNA碎裂定位在勒克司上面、下游基因被放大，在他们之间的PJT10的抗生素的一种抵抗基因被设计进PUC19为构造再结合的plasmidplasmidpUCluxKO。S.mutans房间与的ElectrotransformationpUCluxKO变异导致了抗生素的一种的隔离抵抗S。Mutanstransformants，它被聚合酶链反应，V.harveyiBB170光生物鉴定和定序的分析识别。S的答案。有一样的密度的Mutans标准紧张和勒克司变异的紧张被做并且在pH有教养为一样的period.Terminal生长状况的3.5～7.0BHI液体compared.Firstly在pH被酸化5.5BHI液体，二紧张在pH是有教养的3.0BHI液体。二紧张的酸忍耐回答是compared.Results:限制endonuclease分析证明那pUCluxKO变异的向量成功地被重新结合。S.mutans异种的删除勒克司的地位被PCR与为勒克司和抗生素的一种抵抗的基因特定的教材证实。S.mutans异种不能导致生物体之发光，indiating异种成功地被重新结合。在文化的二十代以后，构造中国S.mutans异种被证实稳定。aciduricity的重要差别在S.mutans标准紧张和标准紧张的抵抗是的勒克司变异的strain.The酸之间被观察比二紧张两个都显示了的勒克司变异的strain.The的强壮酸忍耐回答的能力。结论:S.mutans基因突变而产生之遗传的交换plasmid校正地被构造并且一S.mutans的勒克司否定的异种被构造，它能帮助推进在S.mutans的致病学习勒克司的角色。勒克司变异的紧张对酸忍耐回答的酸inactivation，而是能力更敏感仍然存在。

简介：Objective:Tostudytheeffectofthreedifferentmethodssuchasmedicine,acupunctureandlaseracupunctureonthelevelsofinterleukin2(IL-2),interleukin6(IL-6)andtransforminggrowthfactor-β1(TGF-β1)inrat’sserumwithchronicatrophicgastritis.Methods:9ratswererandomlyselectedfrom60ratsasnormalcontrolgroup,andtheotherratswereusedtoreplicatetheanimalmodelofchronicatrophicgastritiswithcomprehensivemethod,5ratswererandomlyinspectedatthe8thweekintheprocessofcreatingmodelforgastricmucosapathologicalexamination;whengastricmucosashowsCAGsymptomssuchasvariousdegreeofcongestion,bleeding,atrophy,metaplasia,themodelratswererandomlydividedintomodelgroup,medicinegroup,acupuncturegroupandlaseracupuncturegroup.TheratsofmedicinegroupwerechronicallyadministeredwithyanshenjianweicapsuleandKangfuxinLiquid;thegroupsofacupunctureandlaseracupunctureselectedZusanliacupunctureasacupuncturepoints,afterthetreatmentfor14d,bloodwasremoved,doubleantibodysandwichenzymelinkedimmunosorbentassay(ELISA)wasusedtodetectthelevelsofIL-2,IL-6andTGF-β1inserum.Results:Comparedwithnormalgroup,thecontentsofIL-2,IL-6andTGF-β1inserumofmodelratsincreased(P<0.05orP<0.01).Medicine,acupunctureandlaseracupuncturecouldreducethecontentsofIL-2,IL-6andTGF-β1,IL-2inserumofthemedicinegroupandlaseracupuncturegroupwassignificantlydecreased(P<0.05);IL-6inacupuncturegroupandlaseracupuncturegroupweresignificantlydecreased(P<0.05orP<0.01);andthemostsignificantislaseracupuncturegroup(P<0.01).Medicine,acupunctureandlaseracupuncturecouldsignificantlyreduceTGF-β1contentinserum(P<0.05orP<0.01),andtheparticularlysignificantwaslaseracupuncture(P<0.01).Conclusion:Laseracupuncturehasthefunctionofreducingtheinflammatoryresponseandadjustingimmunefunction.ItcaneffectivelyreducetheexpressionlevelofIL-2,IL-6andTGF-β1inserumofratswithchronicat

简介：Interfacedebondingbetweenprosthesesandabutmentswasthemostfrequentfailuremodeofresin-bondedfixedpartialsdentures(RBFPDs)inclinic.ThepurposeofthisstudywastoinvestigatetheeffectofaccessoryretentionformsonthebondstrengthofRBFPDs.Threetypesof3Dfiniteelementmodelswereconstructed.ThemodelofposteriormetalplateRBFPDwithspoon-shapedocclusalrestseatsservedasthecontrol.Theremainingtwotypesofmodelsbasedonthecontroladdedtheretentionformdesignofthepinholeandaxialgrooverespectively.Theaxialorbuccolingualloadof150Nwasappliedontheprosthesis,firstpremolarandfirstmolarrespectively.Themaximumprincipalstressesoftheadhesivelayerindifferentmodelswerecalculated.Undertheloadofthesamemagnitude,thestressduetothebuccolingualloadwassignificantlyhigherthanthatduetotheaxialloadintheadhesivelayer.Theproximalshoulder,occlusalrestseatswallandtheproximalmarginadjacenttotheshoulderwerethehighriskregionwheretheadhesivelayerdamagedeasily.Comparedwiththecontrolmodel,thepinandgroovemodelscouldslightlydecreasethestressintheadhesivelayeraftertheaxialloading,whilethestressintheadhesivelayerdrasticallydecreasedafterhorizontalloadingandreducedby22%and31%respectively.TheseresultsindicatethatthehorizontalocclusalforcehasamoreseriousharmtothedebondingofRBFPDs.Inaddition,theaccessoryretentionforms(e.g.pinandaxialgrooveretentionforms)candecreasethestresslevelintheadhesivelayer,whichareconducivetoincreasetheload-bearingcapacityofRBFPDs.