简介：Cochlearouterhaircells(OHCs)areinvolvedinamechanicalfeedbackloopinwhichthefastsomaticmotilityofOHCsisrequiredforcochlearamplification.Alternatively,amplificationisthoughttoarisefromactivehairbundlemovementsobservedinnon-mammalianhaircells.Wemeasuredthevoltage-evokedhairbundlemotionsinthegerbilcochleatodetermineifsuchmovementsarealsopresentinmammalianOHCs.TheOHCsdisplayedalargehairbundlemovementthatwasnotbasedonmechanotransducerchannelsbutbasedonsomaticmotility.Significantly,bundlemovementswereabletogenerateradialmotionofthetectorialmembraneinsitu.Thisresultimpliesthatthemotility-associatedhairbundlemotionmaybepartofthecochlearamplifier.

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简介：Phaeodactylumtricornutumisoneoftheimportantmarinediatomsforoceanicprimaryproduction.Itsreproductionhasprofoundsignificanceinthelifecycle;however,thenuclearbehaviorduringitssexualreproductionwasnotclear.Inthisstudy,weobservedthenucleartransitionanddetermineditscorrelationwithcellconjunction.Itwasfoundthattwocellsjointedattheirapicesfirstandswungandalignedeachotherimmediately,andnucleusfromonecellwasabletotransferintoanotheroneduringcellconjugation.Thecellpairsconjugatedfornucleartransitionweredifferentfromthoseformedinmitosisinhypovalvethicknessandcellulararrangement.OurfindingsprovedtheexistenceofsexualreproductioninP.tricornutum.

简介：Objective:ToconfirmwhetherBungarusmulticinctuscrudevenominducestheapoptosisofK562tumorcellsandtofindoutthecomponentsinducingapoptosisofK562cellsfromthecrudevenom.Methods:thecrudevenomseparatedandpurifiedbycationexchangechromatography,andtheeffectofvenomsonK562wasstudiedbyMTTmethodandflowcytometry.Results:ThecrudevenombegantokillK562cellsatthan8(103ng/ml(thesurvivalratewas82.5%)concentrationandtheeffectwasmoresignificantin24hwhenadministrating8(105ng/ml(thesurvivalratewas29.4%)crudevenom.ApoptoticbodieswereobservedintheK562tumorcellsbyfluorescentmicroscopyafteradministrationof5(g/mlcycloheximide(CHX)orthepeakVIsolutionatabout8(105ng/ml.Thesameresultsweredetectedbytheflowcytometry.Asub-G1peakappearedafteradministrationofCHXorthesixthpeaksolution.Conclusion:TheauthorsfoundthatthevenomcankillK562tumorcellsintime-anddose-dependentmanner.However,thekillingeffectofthevenomisnotapoptosis.What'smore,thepeakVIsolution,acomponentofthecrudevenomcaninducetheapoptosisofK562tumorcells.

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简介：AbstractBackground:Circular RNA (circRNA) is a type of closed circular noncoding RNA (ncRNA), mostly formed by back-splicing or alternative splicing of pre-messenger RNA (mRNA). The aim of this study was to explore the expression profile of circRNA in peripheral blood mononuclear cells (PBMCs) of patients with ankylosing spondylitis (AS) and discover potential molecular markers of AS.Methods:The circRNA microarray technology was used to detect the expression of circRNAs in the peripheral blood of 6 patients with AS and 6 healthy controls (HC). To screen the differentially expressed circRNAs by fold change (FC) and P value, these differentially expressed circRNAs were analyzed by bioinformatics. In 60 cases of AS and 30 cases of HC, 4 circRNAs were subjected to real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), and their correlation with various clinical indicators was analyzed. Finally, the receiver operating characteristic (ROC) curve was used to analyze their potential as AS diagnostic markers.Results:The microarray results showed that there were 1369 significantly differently expressed (P < 0.05, FC > 1.5) circRNAs between the AS and HC groups (675 upregulated and 694 downregulated). The results of bioinformatics analysis suggested that they were mainly involved in "enzyme binding," "adenosine ribonucleotide binding," "MAPK signaling pathway" , etc. The RT-qPCR results showed that the expressions of hsa_circRNA_001544 (U = 486.5, P < 0.05) and hsa_circRNA_102532 (U = 645, P < 0.05) were significantly different between the AS group and the HC group. The AS group was further divided into two subgroups: active AS (ASA) and stable AS (ASS). After analysis, it was found that compared with the HC group, hsa_circRNA_001544 was significantly increased in both ASA (U = 214, P < 0.05) and ASS groups (U = 273, P < 0.05), while hsa_circRNA_008961 (U = 250, P < 0.05) and hsa_circRNA_102532 (U = 295, P < 0.05) were only significantly increased in the ASA group. Furthermore, hsa_circRNA_012732 was significantly different between the ASA and ASS groups (U = 194, P < 0.05), and there was no statistical significance among the remaining groups. Correlation analysis results showed that hsa_circRNA_012732 was negatively correlated with Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), high-sensitivity C-reactive protein (hsCRP), and globulin (GLOB) and positively correlated with lymphocyte count (LY), mean corpusular volume, and albumin (ALB), and hsa_circRNA_008961 was negatively correlated with platelet (PLT) count. ROC curve analysis showed that hsa_circRNA_001544 (95% CI = 0.610-0.831, P < 0.05) and hsa_circRNA_102532 (95% CI = 0.521-0.762, P < 0.05) were statistically significant, and their area under curve (AUC) values were 0.720 and 0.642, respectively.Conclusions:There are differentially expressed circRNAs in PBMCs of AS patients, and they may be involved in the occurrence and development of AS. Among these differentially expressed circRNAs, hsa_circRNA_012732 has the potential to become an indicator of disease activity, and hsa_circRNA_001544 has the potential to become a molecular marker for AS diagnosis.

简介：AbstractThe effects of gestational diabetes mellitus (GDM) on offspring include macrosomia, hypoglycemia, respiratory distress syndrome, cardiovascular disease, neural and mental injury, etc. The effects of GDM on the health status of offspring are sustained although pregnancy has ended. It has been proposed that fetal reprogramming causes long-term consequences to metabolic health in offspring. An intrauterine high-glucose environment may lead to changes in the multi-differentiation proficiency of intracorporal stem cells, showing decreased proliferation and osteogenic ability, increased adipogenic ability, accelerated apoptosis, and occurrence of premature failure. This environment also reduces the mobilization of bone marrow stem cells, whereas it increases that of medullary cells. This results in pro-inflammatory conditions and sustained inflammation in the body, thereby increasing the risk of obesity, cardiovascular and neurological disorders, and metabolic abnormalities. Stem cells derived from the amniotic membrane, umbilical cord, or placenta may be a reliable predictor of the long-term effects of GDM on offspring. The levels of blood glucose during pregnancy should be effectively controlled to reduce harm to the neonate.

简介：在allogeneic干细胞移植(SCT)，缺乏他们的血缘的禁止的ligand的生来的杀手(NK)房间能导致graft-versus-leukemia回答，没有严重graft-versus-host疾病(GVHD)的正式就职。这个特征能为细胞的免疫疗法被利用。在这研究，我们检验了在整个人的外部血NK房间人口以内表示不同漂亮像免疫球蛋白的受体(KIR)的NK房间子集的选择扩大，面对HLA-Cw3(C1)或Cw4(C2)transfectedK562激发器房间。有C1或C2的KIR+NK房间的Coculture积极K562房间，面对IL-2+；IL-15，触发了错过了他们的血缘的ligand的NK房间的长出。这在整个NK房间人口以内导致了alloreactiveKIR+NK房间的增加的频率。在有缺乏他们的血缘的ligand的K562房间的preculture以后，当时，另外，我们观察到这张alloreactiveNK人口揭示了CD107+房间的更高的数字有相关K562HLA-Ctransfected目标房间的cocultured，作为与有untransfectedK562房间的coculture相比。这提高的反应作为目标用主要白血病的房间被证实。这研究证明那HLA一级表情能调停扭曲NK房间全部剧目并且向白血病的目标房间与提高的alloreactivity为房间充实人口。这个特征可以支持未来NK基于房间的免疫疗法的临床的应用。

简介：Objective:Toobservetheeffectsofthermalstressonproliferationofhumanvascularendothelialcells(VECs)andexploreitssignificance.Methods:ChangesofVECsproliferationwereinvestigatedwith3H-TdRincorporationmethodafterECV304wastreatedat43℃for2hours,whileexpressionsofintercellularadhesionmolecule-1(ICAM-1),inhibitorofdifferentiation-1(ID1),andP16andP21proteinsweredeterminedbyWesternBlotting.Results:TheeffectofinhibitionofVECsgrowthafterthermalstresswasdetectedby3H-TdRincorporationexperiment.WesternblottingshowedICAM-1,amarkerofactivatedendothelialcells,wasincreasedmarkedlyafterthermalstress.ExpressionofID1proteindeclinedgraduallywithincreasingexpressionsofitsdownstreamgenes,P16andP21followingthethermalstress.Conclusions:ThermalstresscouldstronglyactivateVECsandinhibitproliferationofVECsthroughID1,thusdownregulatingcyclin-dependentkinaseinhibitors,P16andP21,whichmightbeanessentialpathwayforrecoveryofVECsafterthermalstress.

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简介：Swelling-activatedCl-currents,I(Cl,swell),weremeasuredduringhyposmoticshockinwhiteLeghornembryonicchickheartcellsusingthewhole-cellrecordingofpatch-clamptechnique.Genistein,aninhibitorofproteintyrosinekinase(PTK),suppressedI(Cl,swell).Underisosmoticconditionphorbol12-myristate13-acetate(PMA),anactivatorofPKC,elicitedtheCl-currentsimilartothatinhyposmoticsolution,whereashyposmoticshockdidnotelicitI(Cl,swell)inchelerythrinechloride(aninhibitorofPKC)-treatedcells.Con-focalmicroscopyexperimentsusingFITC-phalloidinasafluorescentlabelofF-actinshowedthattheactinnetworkwasmovedfromcorticalregionofthecelltothecenterafterhyposmoticshockascomparedwiththeimageunderisosmoticcondition.WhenthecellsweretreatedwithcytochalasinB(CB)orcytochalasinD(CD)underisosmoticconditionthedisruptionoftheF-actinintegritywasobserved,andI(Cl,swell)wasnotelicited.WithcombinationtreatmentofCBwithPMA,hyposmoticsolutioncouldnotelicitedI(Cl,swell).TheresultssuggestedthattheroleofPTK,probablyreceptortyrosinekinase,forregulationofI(Cl,swell)appearedtobeatupstreamsiterelatedtotheroleofF-actin.ThenPKCsignalpathwaywasactivatedsomehowandfinallychangeinthepolymerizationstateofcytoskeletonledtoactivatetheswelling-activatedCl-channels.TheseresultsdemonstrateclearlythatPTK,PKCandF-actinareimportantfactorsforreg-ulationofI(Cl,swell),inembryonicchickheartcellsascomparedwithoftencontroversialresultsreportedindifferentcelltypes.

简介：Thesurfaceofpolypropylene(PP)membranewasmodifiedbylowtemperatureplasmawithammonia.Theeffectofexposuretimewasinvestigatedbymeansofcontactanglemeasurement.Theresultsshowthatlowtemperatureammoniaplcsmatreatmentcanenhanceitshydrophilicity.Chinesehamsterovary(CHO)cellsattachmentonthemodifiedmembranewasenhancedandthegrowthrateonthemembranewasfasterthanunmodifiedone.

简介：AbstractInnate lymphoid cells (ILCs) are a group of lymphocytes without diversified antigen receptors encoded by gene rearrangement on T and B cells. ILCs, which are tissue-resident innate immune cells, expressed particularly in the mucosa or the barrier surface, contribute to the formation of lymphoid organs, the maintenance of tissue homeostasis, and the regulation of antimicrobial defenses. It has been recently reported that ILCs were enriched at the maternal-fetal interface. During a successful pregnancy, the maternal immune system must tolerate a fetus as an allograft. With the new defined of ILCs, a number of studies have shown that three types of ILCs are involved in embryonic development and pregnancy maintenance as well as the occurrence and development of pregnancy-related complications. This article reviews the types and roles of ILCs in normal pregnancy and pregnancy-related diseases.

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简介：ＳＴＵＤＩＥＳＯＮＴＨＥＧＡＰＪＵＮＣＴＩＯＮＡＬＩＮＴＥＲＣＥＬＬＵＬＡＲＣＯＭＭＵＮＩＣＡＴＩＯＮＯＦＨＵＭＡＮＮＡＳＯＰＨＡＲＹＮＧＥＡＬＣＡＲＣＩＮＯＭＡ　ＣＥＬＬＳＡＮＤＴＨＥＥＦＦＥＣＴＯＦＲＩＩＨａｎＬｉｑｕｎ韩立群；ＧａｏＪｉｎ高进；...

简介：AseriesofsamplesdepositedbyVHF-PECVDatdifferentpressureswerestudied.Themeasurementresultsofphotosensitivity(photoconductivity/darkconductivity)andactivationenergyindicatednearthesamerulewiththechangeofthepressure.TheresultsmeasuredbyRamanscatteringspectra,X-raydiffractionandFTIRallprovedtheevidentcrystallizationofthematerials.Treatingthep/iinterfacebyhydrogenhasagreatimprovingeffectontheperformanceofthemicrocrystallinesilicon(μc-Si)p-i-nsolarcellsifthetreatmenttimewasappropriate.Anefficiencyof4.24%forμc-Sip-i-nsolarcellsdepositedbyVHF-PECVDwasfirstlyobtained.

简介：白血病是造血的房间开发的混乱并且被一房间增长和区别解开描绘。当常规处理经常有严重不利副作用，对为白血病治疗的新奇战术的发展有紧迫的需要。Tryptanthrin(6,12-dihydro-6,12-dioxoindolo-(2,1-b)-quinazoline)是从药用的靛青植物(Ban-Lan-Gen)的弄干的根孤立的自然地发生的、弱基本的碱。有被报导了各种各样生物并且药理学活动包括抗菌剂，反煽动性，immunomodulatory和反肿瘤效果。然而，它房间糟糕仍然是的myeloid上的调节效果和行动机制理解。在这研究，tryptanthrin被显示在一个剂量依赖者和时间依赖者举止压制鼠科的myeloid白血病WEHI-3BJCS房间的增长。它显著地也在syngeneicBALB/c老鼠在vivo减少了WEHI-3BJCS房间的生长。然而，它没在正常的鼠科的腹巨噬细胞上展出重要直接cytotoxicity。流动cytometric分析在G0/G1阶段显示出对待tryptanthrin的WEHI-3BJCS房间的明显的房间周期拘捕。cyclinD2的表示，D3，Cdk2，在WEHI-3BJCS房间的4和6基因被发现在由RT-PCR测量了的24h下面调整。词法、功能的研究表明tryptanthrin能在WEHI-3BJCS房间导致区别，由vacuolation的增加出现在对待tryptanthrin的房间的细胞的颗粒度和减少NBT活动。一起，我们的调查结果建议tryptanthrin可能由引起房间周期拘捕并且由触发房间区别在鼠科的myelomonocytic白血病WEHI-3BJCS房间上施加它的反肿瘤效果。

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简介：TheeffortonelectrochemicalreductionofCO2tousefulchemicalsusingtherenewableenergytodrivetheprocessisgrowingfastrecently.Inthisreview,weintroducetherecentprogressesontheelectrochemicalreductionofCO2insolidoxideelectrolysiscells（SOECs）.Athightemperature,onlyCOisproducedwithhighcurrentdensitiesandFaradicefficiencywhilethereactoriscomplicatedandabettersealingtechniqueisurgentlyneeded.Thetypicalelectrolytessuchaszirconia-basedoxides,ceria-basedoxidesandlanthanumgallates-basedoxides,anodesandcathodesareintroducedinthisreview,andthecathodematerials,suchasconventionalmetal–ceramics（cermets）,mixedionicandelectronicconductors（MIECs）arediscussedindetail.Inthefuture,togainmorevalue-addedproducts,theelectrolyte,cathodeandanodematerialsshouldbedevelopedtoallowSOECstobeoperatedattemperaturerangeof573–873K.Atthosetemperatures,SOECsmaycombinetheadvantagesofthelowtemperaturesystemandthehightemperaturesystemtoproducevariousproductswithhighcurrentdensities.