简介：ＴＨＥＰＲＯＰＥＲＴＩＥＳＯＦＬＥＵＫＯＣＹＴＥＡＮＤＥＲＹＴＨＲＯＣＹＴＥＡＤＨＥＲＥＤＴＯＥＮＤＯＴＨＥＬＩＡＬＣＥＬＬＩＮＦＬＯＷＦＩＥＬＤＴＨＥＰＲＯＰＥＲＴＩＥＳＯＦＬＥＵＫＯＣＹＴＥＡＮＤＥＲＹＴＨＲＯＣＹＴＥＡＤＨＥＲＥＤＴＯＥＮＤＯＴ...

简介：Objective:Thisstudyaimstoexploretheclinicopathologicfeaturesof112patientswithmantlecelllymphoma(MCL).Methods:Datafrom112MCLcaseswerecollected,andimmunohistochemicalassaywasconducted.Fluorescenceinsituhybridization(FISH)detectedabreakintheCCND1gene.Thet-testwasusedinthestatisticalanalysis.Results:Alltumorcellsinthe112casesexpressedBcell-relatedantigen,including1blastoidsubtypeand1polymorphicsubtype.Amongallcases,106expressedCD5and104expressedcyclinD1.AbreakintheCCND1genewasnotfoundin3caseswithCD5-MCL.IgH/CCND1polyploidwasobservedin2classiccases.Conclusion:MCLisatypeofspecialimmunophenotypicB-celllymphoma.Theprognosesofblastoidandpolymorphicsubtypesarepoor.Specialsubtypesshouldbeclassifiedduringdiagnosis.

简介：Stemcellshavetheremarkablepotentialtodevelopintomanydifferentcelltypes,essentiallywithoutlimittoreplenishothercellsaslongasthepersonoranimalisstillalive,offeringimmensehopeofcuringAlzheimer’sdisease,repairingdamagedspinalcords,treatingkidney,liverandlungdiseasesandmakingdamagedheartswhole.Untilrecently,scientistsprimarilyworkedwithtwokindsofstemcellsfromanimalsandhumans:embryonicstemcellsandnon-embryonic'somatic'or'adult'stemcells.Recentbreakthroughmakeitpossibletoconvertor'reprogram'specializedadultcellstoassumeastemstem-likecellswithdifferenttechnologies.Thereviewwillbrieflydiscusstherecentprogressesinthisarea.更多还原

简介：Objective:TodetectdifferentialproteinexpressioninmalignantandnormallivercelllinesinvitrousingtheSELDIProteinChipplatform,forinvestigatingthepathogenesisoflivercancer.Methods:Twocelllines,humannormallivercelllineL02andhepatomacelllineSMMC-7721wereculturedroutinely,harvestedingoodconditionandlysed.Afterquantification,thesupernatantofthelysatewastestedbyIMAC3(ImmobilizedMentalAffinityCapture)andWCX2(WeakCationExchange)chipsontheSELDI-TOF-MSProteinChipreader.Results:Proteinexpressiondifferedbetweenthemalignantandnormallivercelllines.Atotalof20differentiallyexpressedproteinswerefound,amongwhich,7werecapturedbytheIMAC3chipand14bytheWCX2chip.Peaksat5,419,7,979and11,265Dawerehigherandat8,103,8,492,10,160and11,304DalowerinSMMC-7721cellsbytheIMAC3chip;peaksat7,517,7,945and7,979Dawerehigherandat5,061,5,551,5,818,7,439,9,401,10,100,10,312,11,621,11,662,11,830and12,772DalowerinSMMC-7721cellsbytheWCX2chip.Interestingly,bothchipscapturedthe7,979Dapeak.Inaddition,the11,081DapeakcorrespondedpreciselywiththemolecularmassofthecalciumbindingproteinS100A10,whichmayparticipateintheformationoflivercancerinassociationwithp36.Conclusion:DetectingdifferentialproteinexpressioninmalignantandnormallivercelllinesusingtheSELDIProteinChipplatformwassimple,sensitiveandrepeatable.Theresultsweobtainedcanserveasabasisforinvestigatingthepathogenesisoflivercancerandaidthediscoveryofnewtherapeutictargets.

简介：Itiswellestablishedthatstemcellscandifferentiateintocelltypesoftheorganinwhichthesearetransplanted.However,theprocessisveryslowduetolackofunderstandingofsignalsimportantfortheirsurvivalanddifferentiation,mostoptimalstemcellsandtheirplasticity.Limitationsandadvantagesofvariouscellsubtypeswillbedescribed.Therateofstemcellsmobilizationandtheirsurvivalintheischemicenvironmentaremajorobstaclesinengraftmentanddifferentiationofstemcellsformeaningfulrepairoftheinfarctedmyocardium.Manipulationofstemcellswithischemicpreconditioning,combinedgeneandcelltherapytogetherwithsimultaneousactivationofdiversesignalingpathwaysformassivestemcellmobilization®enerationhassignificantimpactontherepairprocessbystemcells.Theseandotherdifficultiesencounteredinefficientuseofvariousstemcellshaveresultedininventionofinducedpluripotentstemcellswhichcouldrevolutionizethestemcellbasedtherapyandtheirapplicationsforunderstandingofhumandiseaseanddrugscreeninginthenearfuture.ReprogrammingofadultcellsintoiPScellswithouttheuseofviralvectorsisamajorchallengetowardsgettingiPScellswithoutviralintegrationintocells.Tomeetthischallengewehaverepro-grammedskeletalmyoblastsintoiPScellswithhighefficiencyusingepigeneticmodifiers.TransplantationofiPScellsderivedpurecardiacprogenitorsintoinfarctedmyocardiumledtoextensiverepopulationofscarareawithfullydevelopedmyocyteswithouttumorformationandresultinginmarkedimprovementincardiacfunction.Reprogrammingwithpurechemicalmeanswillmaketherapeuticuseofthesecellsmoresafer.Targetingtheinducedpluripotentstemcellstowardscardiacprogenitorsandtheirapplicationtowardstransplantationisamajorstepforwardinenhancingthemyocardialrepaircapacitybythesecells.

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简介：AbstractHistorically, breast cancer has been regarded as an immunogenic "cold" tumor. However, the discovery of immune checkpoint inhibitors has made immunotherapy becoming an emerging new treatment modality for breast cancer. This review discusses the immune system, immune features of breast cancer, and the programmed cell death protein-1/programmed cell death protein ligand-1 (PD-1/PD-L1) inhibitors used in the treatment of breast cancer. High T lymphocyte infiltration and mutation burden were observed in triple-negative breast cancer and human epidermal growth factor receptor 2 positive breast cancer. Increasing breast cancer immunogenicity and modulating the tumor microenvironment has been reported to improve the therapeutic efficacy of immunotherapy. Recent clinical trials involving PD-1/PD-L1 inhibitors monotherapy in breast cancer has revealed little efficacy, which highlights the need to develop combinations of PD-1/PD-L1 inhibitors with chemotherapy, molecularly targeted therapies, and other immunotherapies to maximize the clinical efficacy. Collectively, the immunotherapy might be a promising therapeutic strategy for breast cancer and several clinical trials are still on-going.

简介：　　小刘跟主任到W市出差,抽空到服装市场去转了转.W市的服装市场远近闻名,不光是因为这个市场大,而是因为这里的服装全,听人介绍,这里各式各样的世界名牌服装都有.……

简介：A在与人的交往之中,有时会出现一个奇怪的规律。比如一个时期结识的朋友都姓李,这些北鞑南蛮的大李小李彼此不认识,但神秘地有着一刀切齐的共性:穷、倒霉、命不好。害得我——可是我又能有多大本事帮别人呢?害得我费了不少想帮人的心思。过了些年,有一天无意中掐指一数,咦,朋友变了!

简介：本期我们来认识另一组辅音－/t/＆/d/。发这两个音时，先将双唇微微张开，舌尖抵住上齿龈，然后稍稍用力将舌尖弹开。

简介：摘要：CPR核电机组大修期间，根据机组安全相关系统和设备定期试验监督大纲的要求，需要执行T RIS 001以及T EIE 001试验，以分别验证整个安全注入顺序和安全壳隔离阶段A顺序运行良好、整个安全壳喷淋顺序和安全壳隔离阶段B顺序的运行符合要求。本文对T RIS 001以及T EIE 001试验进行了简单介绍，探讨了试验优化的需求和优化方案。

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简介：AIMTo在角膜的endothelial房间(CEC)并且到的增长上探索调节媒介的效果比较不同调节媒介(厘米)的效率.METHODSRatCEC，角膜的stromal房间(CSC)，骨头导出髓的endothelial祖先房间(BEPC)，并且骨头导出髓的间充质的干细胞(BMSC)被孤立并且在vitro有教养。厘米从CSC，BEPC，和BMSC被收集。CEC在不同文化媒介被栽培。房间形态学被记录，并且基因和蛋白质表示是为5d在厘米种的analyzed.RESULTSAfter，在每个试验性的组的CEC仍然保持多角形，在像鹅卵石的单层安排。Immunocytofluorescence揭示了Na+/K+-ATP,aquaporin的积极表示1(AQP1)，并且zonulaoccludens1(ZO-1)。把分析基于量的聚合酶链反应(qPCR)，在CSC厘米的Na+/K+-ATP表示是尤其是由1.3褶层的upregulated(±；0.036)(P<；0.05，n=3)。ZO-1的表示层次，神经原特定的enolase(NSE)，Vimentin，配对的homebox6(PAX6)，并且procollagen类型VIII(COL8A1)是尤其是在每个试验性的组的upregulated。每厘米在CEC增长上有积极效果，并且CSC厘米在proliferation.CONCLUSIONCSC厘米，BEPC厘米，和BMSC厘米上有最强壮的效果不仅刺激了CEC的增长，而且坚持说特征区分了为endothelial功能必要的显型。CSC厘米在CEC增长上有最著名的效果。

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简介：AIM:Heatshockprotein(HSP)70isover-expressedinhumangastriccancerandplaysanimportantroleintheprogressionofthiscancer.WeinvestigatedtheeffectsofantisenseHSP70oligomeronhumangastriccancercelllineSGC-7901,anditspotentialroleingenetherapyforthiscancer.METHODS:HumangastriccancercelllineSGC-7901wastreatedinvitrowithvariousconcentrationsofantisenseHSP70oligonucleotidesatdifferentintervals.Growthinhibitionwasdeterminedaspercentagebytrypanbluedyeexclusiontest.ExtractedDNAwaselectrophoresedonagarosegel,anddistributionofcellcycleandkineticsofapoptosisinductionwereanalyzedbypropidiumiodideDNAincorporationusingflowcytometry,whichwasalsousedtodetecttheeffectsofantisenseoligomerpretreatmentonthesubsequentapoptosisinducedbyheatshockinSGC-7901cells.ProteinswereextractedforsimultaneousmeasurementofHSP70expressionlevelbySDS-PAGEWesternblotting.RESULTS:Thenumberofviablecellsdecreasedinadoseandtime-dependentmanner,andladder-likepatternsofDNAfragmentswereobservedinSGC-7901cellstreatedwithantisenseHSP70oligomersataconcentrationof10μmol/Lfor48hor8μmol/Lfor72h,whichwereconsistentwithinter-nucleosomalDNAfragmentation.Flowcytometricanalysisshowedadose-andtime-dependentincreaseinapoptoticratebyHSP70antisenseoligomers.ThisresponsewasaccompaniedwithadecreaseinthepercentageofcellsintheG1andSphasesofthecellcycle,suggestinginhibitionofcellproliferation.Inaddition,flowcytometryalsoshowedthatpretreatmentofSGC-7901cellswithHSP70antisenseoligomersenhancedthesubsequentapoptosisinducedbyheatshocktreatment.WesternblottingdemonstratedthatHSP70antisenseoligomersinhibitedHSP70expression,whichprecededapoptosis,andHSP70wasundetectableattheconcentrationof10μmol/Lfor48hor8μmol/Lfor72h.CONCLUSION:AntisenseHSP70oligomerscanabrogateHSP70expressioninSGC-7901cells,wh

简介：ThepresentstudyisaimedatstudyingthegeneforTIMP-3,amammaliantissueinhibitor,byconstructingarecombinanteukaryoticcellvectorforgenetherapyinhumanbreastcancer.WeobtainedtheTIMP-3genefromthehumanplacentbyRT-PCR.TIMP-3genewassubclonedintopcDNA3.1vetorfrompMD18TvectorbymeansofgenecloningtoconstructpcDNA3.1recombinantvector.HumanbreastcancercelllineMDA-MB-453wastransfectedwithpcDNA3.1-TIMP3recombinantvectorusinglipofectaminereagent.ThentheexpressionofTIMP-3andtheeffectonthemetastasisofMDA-MB-453wereexamined.ThecorrectconstructionofpcDNA-TIMP3wasidentifiedbymeansofrestrictionenzymeanalysis,PCRamplicationandnucleotidesequencing.WesternblottingshowedthatthetransfectedcellswereabletoexpressTIMP-3,indicatingthatourconstructionofthepcDNA-TIMP3eukaryoticexpressionvectorwasconstructedsuccessfully.OurexperimentsfurtherindicatedthatthepotentialofmetastasiswassignificantlyreducedforthetransfectedcelllineMDA-MB-453.

简介：讨论了方程ｘ（ｔ）＝－ｘ＾３（ｔ－１）－ｘ＾３（ｔ－２）周期解的结构。

简介：TheanalysisofcelllossratesinanATMMuxwithlossprioritiesisanimportantprobleminthe-studyoftrafficcontrolinATMnetworks.Inthispaper,thelossratesofthecellswithdifferentprioritiesinanATMMuxareanalyzedbyapproximatingtheactualinputprocesswithtwo-stateMMDPandfluidflowtechnique,andtheanalyticalexpressionsoftherelationbetweenthelossratesandthebuffersizeareobtained.Simulationshowsthattheapproachissullicientlyaccurateforapplications.

简介：Objective:Signetringcellcarcinomaisararesubtypeofcolorectalcarcinoma(CRC)withanassociatedBRAFV600Emutation.WeinvestigatedfrequenciesofBRAFmutationin28CRCscontainingvariablesignetringcellcomponentandtheirrelationwithclinicopathologicparameters.Methods:Accordingtothepresenceofsignetringcellcomponent,tumorswerecategorizedintogroupsasfollows:0%–9%,10%–24%,25%–49%,and>50%.GenomicDNAwasisolatedandanalyzedforBRAFV600Egenemutationbypolymerasechainreaction-restrictionfragmentlengthpolymorphism.Elevenof28cases(39.3%)showedBRAFV600Emutation,whichwasalsoconfirmedbySangersequencing.Toelucidatetheimportanceofexistenceofsignetringcellcomponentatthemolecularlevel,weseparatedcasesintotwogroupswithcut-offlevelsof10%and50%,whichpertaintopercentagesofsignetringcells.Results:Sevenof19cases(36.8%)underthethresholdof50%andfourofninecases(44.4%)overthisthresholdvaluedemonstratedBRAFmutation.Threeof7cases(42.8%)featuring<10%signetringcellcomponentandeightoutof21cases(38.1%)showing>10%wereBRAFmutated.Conclusions:BRAFmutationmustbecloselyassociatedwiththepresenceofmalignantsignetringcellsregardlessoftheirpercentages.

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