简介：Domesticpig(Susscrofadomestica)isoneofthemostimportantmammalstohumans.Alternativesplicingisacellularmechanismineukaryotesthatgreatlyin-creasesthediversityofgeneproducts.Expressionsequencetags(ESTs)havebeenwidelyusedforgenediscovery,expressionprofileanalysis,andalternativesplicingdetection.Inthisstudy,atotalof712,905ESTsextractedfrom101differentnon-normalizedESTlibrariesofthedomesticpigwereanalyzed.TheseESTlibrariescoverthenervoussystem,digestivesystem,immunesystem,andmeatproductionrelatedtissuesfromembryo,newborn,andadultpigs,makingcontributionstotheanalysisofalternativesplicingvariantsaswellasexpressionprofilesinvariousstagesoftissues.AmodifiedapproachwasdesignedtoclusterandassemblelargeESTdatasets,aimingtodetectalternativesplicingtogetherwithESTabundanceofeachsplicingvariant.Mucheffortsweremadetoclassifyalternativesplicingintodifferenttypesandapplydifferentfilterstoeachtypetogetmorereliableresults.Finally,atotalof1,223geneswithaverage2.8splicingvariantsweredetectedamong16,540uniquegenes.Theoverviewofexpressionprofileswouldchangewhenwetakealternativesplicingintoaccount.

简介：Animprovedmethod,calledAlternativeSpectralRotation(ASR)measure,forpredictingproteincodingregionsinriceDNAhasbeendeveloped.ThemethodisbasedontheSpectralRotation(SR)measureproposedbyKotlarandLavner,anditsaccuracyishigherthanthatoftheSRmeasureandtheSpectralContent(SC)measureproposedbyTiwarietal.Inordertoincreasetheidentifyingaccuracy,wechosethreedifferentcodingcharacters,namelytheasymmetric,purine,andstop-codonvariablesasparameters,andanapprovingresultwaspresentedbythemethodofLinearDiscriminantAnalysis(LDA).