简介：Osteoblastsofratculturedinvitrowerestimulatedwithpulsed50Hzelectromagneticfieldandbasicfibroblastgrowthfactor(bFGF).TheMTTmethod,flowcytometryandhistochemistrystainingwereusedtodetectcellproliferation,cellcycleandalkalinephosphatase.Theresultsindicated:afterstimulatedby1mTelectromagneticfield,thecellsaremoreabundant,havemoreSphasepercentages,2mTelectromagneticfieldhavenoevidenteffectoncells'growth;comparedwithelectromagneticfield,thecellsstimulatedbybFGFaremoreabundantandhavelargerSphaseratios.ElectromagneticfieldandbFGFhavenoeffectoncells,alkalinephosphatase.Therefore,weconcludedthatelectromagneticfieldcanenhanceosteoblastsgrowthlikesomegrowthfactorsuchasbasicfibroblastgrowthfactor,andtheosteoblasts',characteristicswasnotchanged.

简介：INTRODUCTIONThedisease,damageorlossoftissueandorgancanseriouslyaffectthehumanlife,evenleadstomaimedordeath,thatitshouldnotbeignored.Todate,autograftsandallograftstransplantationarethemostusuallyusedmethodstorepairdefectsoftissuesandorgans.

简介：客观：在牛的心包的非细胞组成的新鲜标本在endothelial细胞(Ecs)的粘附和增长上调查脉管的endothelial生长因素(VEGF)和它的效果的绑定和版本，它被heparinization.Methods修改:牛的心包的Cross-linked非细胞组成的新鲜标本是由三个方法的heparinized:(1)heparinizedN-(3-dimethylaminopropyl)-N'-ethylcarbodiimidehydrochloride(EDC)对待非细胞组成的织物样品；(2)(ethyleneimine)(PEI)heparinizedpoly对待

简介：Guidedtissueregenerationisanewapproachinthereconstructivesurgeryofperipheralnerves.Biomimeticconductswereconstructfromtheexpandedveinonwhoseinnersurfacecompositedwithamnionfilaments(cf.Fig1).

简介：IntroductionAsamemberofthebonemorphogeneticprotein(BMP)family,BMP-2playsimportantrolesnotonlyinboneregenerationandbonerepairbutalsoincellproliferation,apoptosis,differentiationandmorphogenesis.TheBMP-2remarkableabilitytostimulatenewbonegrowthresultsinthedevelopmentofanoveltherapystrategyforbonemassdefectduetoaccidentsordiseases.BecausetheBMP-2itself,inconjunctionwithasuitablematrix,issufficienttostimulategenesisofnewbone,thegeneticallyengineeredBMP-2hasgoodappliedprospects.

简介：Objective:ThisstudyaimstoexploretheassociationbetweenthedensityofHimalayanmarmot(Marmotahimalayana)andclimaticfactorssuchastemperature,precipitation,humidity,vapourpressure,sunshinepercentage,windvelocity,whicharecloselyassociatedwithglobalclimatechange,andtoprovideareferenceforplaguepreventionandcontrol.Methods:WeconductedaregressionanalysistofindthepossibleclimatefactorsassociatedwiththedensityofHimalayamarmot,andanalyzedtheresponsecharactersofHimalayanmarmottoclimatechange.Results:Dailyprecipitationdays(>=0.1mm)andsunshinepercentageweresignificantlyassociatedwiththedensityofHimalayanmarmot(p<0.01).Conclusion:Climatechangewasassociatedwiththeriskofplague.ThisphenomenonisvaluableforHimalayanmarmotandplagueprevention.MorestudiesareneededtounderstandtheimpactofclimatechangeonHimalayanmarmotandplague.

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简介：Thestressanalysisofthehumanfemurisconsiderablycomplicated.Inadditiontothemotionalpostureandtheloadingconditionofthefemur,itisgreatlyrelatedtoitsgeometryandbonystructureindifferentparts.Inpreviousresearches,themeansoffemoralshapeusinginthefinite-element-analysiswasobtainedbymeansofapply-ingtheradiographswhichweretheninputincomputer.Obviously,theresultswereneitherrealnorprecise.Inthispaper,thecomputerimageprocessingwasusedtogetthegeometryandthesizeofthehumanfemur,whichcouldmakethefinite-ele-

简介：Theergonomicdigitaldesignandevaluationofman-machinesystemisthemainapplicationfieldsofvirtualhumantechnology.Becauseofthecomplexityofphysicalpersonanduncertaintyoffatiguegenerationmechanism,itisdifficulttoexpressandevaluatetheVirtualHuman'sfatiguestate.Thispapersummarizesthecharacteristicsofvirtualhuman'sfatigueevaluation,suchasundeterminable,invisible,theconstraintconditionscanbeenused.Viewedfromthestrengthsandweaknessesofpreexistingmodels,metabolicenergyexpendituremodel,dynamicmusclefatiguemodelandamodeltoassessfatigueatjoint-levelusingthehalf-jointconcept,thispaperputsforwardthefuturedevelopmenttrendthatthecombinationofsubjectiveandobjective,diversificationoftheanalyzablemotion,real-timeandvisualexpression.

简介：ＤＩＥＬＥＣＴＲＩＣＰＲＯＰＥＲＴＩＥＳＯＦＨＵＭＡＮＢＲＥＡＳＴＣＡＲＣＩＮＯＭＡＡＮＤＳＵＲＲＯＵＮＤＩＮＧＴＩＳＳＵＥＳＤＩＥＬＥＣＴＲＩＣＰＲＯＰＥＲＴＩＥＳＯＦＨＵＭＡＮＢＲＥＡＳＴＣＡＲＣＩＮＯＭＡＡＮＤＳＵＲＲＯＵＮＤＩＮＧＴＩＳＳＵＥ...

简介：Objective:Toachieveanoptimizedmethodforsolubleexpressionofhumancarboxylesterase1(hCE-1)inescherichiacoilandpurificationbyNi2+-NTAagaroseaffinitychromatography,togetimprovedproteinyieldandpurityforfurtherdevelopmentofhepatocellularcarcinoma(HCC)diagnosisELISAkits.Methods:ThebestantigenepitopesofhCE1werepredictedbycomparingsecondarystructure,flexibleregions,hydrophilicity,antigenicindexsurfaceprobabilityofresidues.Afterwards,pET-42a(+)withaHis-tagandaGST-tagwasappliedtoformrecombinantplasmidpET-42a(+)/hCE1,whichfacilitatedpurificationwhenusingNi2+-NTAagaroseaffinitychromatography.ProteinqualitywasmeasuredbySDS-PAGEandBCAproteinassay.Western-blotidentificationwasalsoperformedtoensurethecorrectexpressionofhCE1protein.Results:Theresiduesfrom500to567nearC-terminalofhCE1proteinwereconsideredthebestepitopeswhichexhibitedhighhydrophilicityandhighsurfaceprobabilityandrelativelyflexiblesecondarystructureandlowhomologycomparedwithhCE2andhCE3.His-hCE1500-567fusionproteinwasachievedbyIPTG-inductedexpressionwithanexpectedmassof42kDa.Afterpurification,thefinalproductwasspeciallyidentified,whichreachedover95%purityandmorethan10mg/Lofmicrobialculture.InWesternblot,thepurifiedfusionproteinwasrecognizedbyanti-hCE1monoclonalantibody,alongwithprevioussequencingvalidation,whichdemonstratedthecorrectpreparationofsolublehCE1protein.Conclusion:ThisisanefficaciousandaffordablestrategytogeneratefusionhCE1ofhighqualityinEcoli,whichfacilitatespreparationofhCE1monoclonalantibodyandfurtherHCCdiagnosisresearch.

简介：Theapplicationofhumanhomografthasbeenpopularincardiovascularsurgeryandshownperfecteffects.Butthedifficultytogetgraftgreatlylimiteditsclinicalap-plication.Thethawedhomograftcannotbeusedinsomecasessinceitisnotclearwhetheritcanbere-cryopreservedwithoutdamage.Weconductedthepresentstudytoknowthechangesinviabilityofthehomograftafterrefreezinginsixhumanpul-

简介：ＰＲＥＬＩＭＩＮＡＲＹＲＥＳＥＡＲＣＨＯＦＨＵＭＡＮＨＥＰＡＴＯＣＹＴＥＣＥＬＬＬＩＮＥＡＳＴＨＥＢＩＯＬＯＧＩＣＡＬＭＡＴＥＲＩＡＬＯＦＥＬＡＤＰＲＥＬＩＭＩＮＡＲＹＲＥＳＥＡＲＣＨＯＦＨＵＭＡＮＨＥＰＡＴＯＣＹＴＥＣＥＬＬＬＩＮＥＡＳＴＨＥＢＩＯ...

简介：Withaself-madenear-infraredanalyticalinstrumenttobloodandoxygenparametersinhumanbrain,80casesinwhich20arehealthypersonsand30areanaesthetisedcasesandothersarepatientswithheartfunctionlackistakentoexamine,andthedataofbloodandoxygeninbraintissuewerecollectedandanalyzedbythemethodofpowerspectrumandcorrelationfunction.Theresultsindicatethat:(1)Theaveragebrainoxygensaturationofhealthypersonsandanaesthetisedcasesisabout80%,inaccordwithnormalparameterofphysiology.Contrastively,theaveragebrainoxygensaturationofpatientswithheartfunctionlackis72.8%,whichisobviouslylessthanthatofhealthypersonsandanaesthetisedcases.Theprobabilityofmedicalstatisticsislessthan0.01.(2)Theshapesofwaveofbrainbloodandoxygenforthehealthypersonandtheanaesthetisedcaserevealsmallperiodicalfluctuationswithstableshapeandbaseline,andthetrendofincreaseordecreaseofbloodandoxygenparametersinbraintissueissynchronousandaphasereversal,butforthepatientwithheartfunctionlackinabrainoxygenlackstate,theshapesofwaveareirregular.Thisisahintthatnearinfraredlightpassingthroughtissuecanreflecttheintuitionisticchangeofbrainbloodandoxygenparameters.(3)Thepowerspectraofbrainbloodandoxygenforthehealthypersonandtheanaesthetisedcasehasaclearmainpeak,narrowbandwidthandperfectsuperpositioneachother,butthepowerspectraforthepatientwithheartfunctionlackinabrainoxygenlackstateisonthecontrary.(4)Theaveragecrosscorrelationcoefficientofbrainbloodandoxygenforhealthypersonsandanaesthetisedcasesis-0.9825±0.1027closeto-1.Buttheaveragecrosscorrelationcoefficientforpatientswithheartfunctionlackinabrainoxygenlackstateismerely-0.8923±0.1035whichisobviouslygreaterthan-1andtheprobabilityofmedicalstatisticsislessthan0.01.Theclinicexperimentshaveprovedthattheshapes

简介：Thecompressedsensing(CS)ofaccelerationdatahasbeendrawingincreasingattentioningaittelemonitoringapplication.Insuchapplication,therestillexistsomechallengingissuesincludinghighenergyconsumptionofbody-worndeviceforaccelerationdataacquisitionandthepoorreconstructionperformanceduetononsparsityofaccelerationdata.Thus,thenovelschemeofcompressivesensingofaccelerationdataisneededurgentlyforsolutionsthatarefoundtotheseissues.

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简介：ＥＦＦＥＣＴＳＯＦＡＰＡＴＩＴＥＣＥＲＡＭＩＣＳＯＮＣＥＬＬＧＲＯＷＴＨＳＡＮＤＤＮＡＳＹＮＴＨＥＳＥＳＩＮＨＵＭＡＮＯＳＴＥＯＢＬＡＳＴＬＩＫＥＣＥＬＬＥＦＦＥＣＴＳＯＦＡＰＡＴＩＴＥＣＥＲＡＭＩＣＳＯＮＣＥＬＬＧＲＯＷＴＨＳＡＮＤＤＮＡＳＹＮＴＨ...

简介：ＬＩＧＨＴＳＣＡＴＴＥＲＩＮＧＰＲＯＰＥＲＴＹＯＦＨＵＭＡＮＢＬＯＯＤＡＴＴＨＥＷＡＶＥＬＥＮＧＴＨＯＦ８１０ＮＭＬＩＧＨＴＳＣＡＴＴＥＲＩＮＧＰＲＯＰＥＲＴＹＯＦＨＵＭＡＮＢＬＯＯＤＡＴＴＨＥＷＡＶＥＬＥＮＧＴＨＯＦ８１０ＮＭＪｉａｎＺｈｏｎｇ；Ｄ...

简介：MatthiasLtitolfandhisPhDstudent,twoscientistsattheEPFL＇sInstituteofBioengineering,havedevelopedanewtechniqueforusinglaserstocarvepathwaysinhydrogelscaffoldsina3Dcellcultureenvironmenttocontrolthegrowthoftheculture.

简介：Osteochondrosisdissecans(OD)ofthehumankneeisanasepticprocessofsub-chondralbonenecrosiswhichoftenleadstothepartialorcompleteseparationofasubchondralfreeparticlecoveredwitharticularcartilage.Epidemiologicalstudieshaverevealedthatincidentalpeakofageconcentrateintheseconddecadeoflife,es-peciallyinhighlyexercisedpopulationsuchasathletes.Thelocationoftheinvolved