简介：Objective:Tostudytheexpressionofactivatedepi-dermalgrowthfactorreceptor(EGFR)andtranscrip-tionfactorE2F(E2F)inCondylomaAccuminata(CA)patients.Methods:ImmunofluorescenttechniqueswereusedtoinvestigatetheexpressionofactivatedEGFRandE2FinCApatients.Results:TheexpressionofactivatedEGFRonthemembraneofepithelialcellsinCAlesionswassig-nificantlygreatercomparedtoexpressionleversinthecontrolgroup(P<0.01).Moreover,theco-expres-sionofactivatedEGFRandE2Fwassignificantlyin-creasedcomparedtothecontrolgroup(P<0.01).Conclusion:Ourobservationssuggestthatthein-creaseinactivatedEGFRexpressionmaystimulatehyperplasiainCApatientsthroughtheactivationoftranscriptionfactorE2F.

简介：Objective:Tostudytheexpressionlevelsofplatelet-derivedgrowthfactor(PDGF)andgranulocytecolony-stimulatingfactor(G-CSF)inperipheralbloodandtheirroleinthepathogenesisofCondylomaacuminatum(CA).Methods:Seraweretakenfrom70patientswithCondylomaacuminatumandcomparedwith35healthycontrols.PDGFandG-CSFinserumwerequantitatedusingadualantibodysandwichenzyme-linkedimmunoabsorbentassay(ELISA).Results:SerumconcentrationsofPDGFandG-CSFweresignificantlyincreasedinpatientswithCondylomaacuminatum(CA)comparedtocontrols(P<0.001andP<0.005respectively).SerumlevelsofPDGFandG-CSFcorrelatedwithclinicalseverityofCA,butnosignificantdifferencewasobservedbetweendifferentdurationofdiseasegroups.AsignificantpositivecorrelationwasnoticedbetweenneutrophilcountandG-CSFlevels(γ=0.38,P<0.001),andtheneutrophilcountshowednosignificantcorrelationwithPDGF.Conclusion:TheresultsindicatedthatincreasedexpressionofPDGFandG-CSFinperipheralbloodmightbeinvolvedinpathogenesisofCA.

简介：Objective:ToinvestigatetheexpressionofHPV16mRNAinnormalhumankeratinocytestransfectedwithpSV2-neo/16.Firsthumankeratinocyteswereculturedintheserum-freemediumM154.Second,theplasmidpSV2-neo/16wastransfectedintothehumankeratinocytesusingatransfectingreagent.Third,RT-PCRandSouthernBlottingwereusedtodetecttheexpressionofHPV16mRNAandDNAinthetransfectedkeratinocytes,respectively.Results:TheexpressionofHPV16mRNAwassuccessfullyamplifiedandan110bpwasditectedbyRT-PCR.A7.9kbfragmentwasconfirmedinthetransfectedkeratinocytesbySouthernBlotanalysis.Conclusion:HPV16mRNAandDNAweresuccessfullydetectedinthehumankeratinocytes.

简介：Summary:RiskfactorsforgenitourinaryChlamydiatrachomatis(Ct)infectionwereinvestigatedusinganSTDrelativeriskfactorquestionnaireamong176patientsandtheirregularsexualpartners.Twenty-fourindependentvariableswereselectedforanalysis,nineofwhichwereconfirmedasfactorsassociatedwithC.trachomatis.Fouroftheninefactorsweresignificantlycorrelatedtoinfectionusingamultifactorialunconditionallogisticregressionmodelwhichincluded:levelofeducation(oddsratios[ORs]:2.144forbelowjuniormiddleschool),numberofsexualpartners(ORs:4.503for≥5),numberofregularpartner'ssexualpartner(ORs:16.333for≥5),STDhistoryofregularpartner(ORs:18.417fortheirSTDhistory).Thesedatademonstratethatregularpartner'ssexualbehaviorsarealsoanimportantriskfactorforCtinfectionamong,STDclinicclients.

简介：Abstract:Thisstudyinvestigatedtherelationshipbetweenhumanpapillomavirus(HPV)genotypeandexpressionofp53andp21^WAH1.Expressionofp53andp21^WAH1in35casesofcondylomaacuminatumspecimensinfectedbyHPV6/llandHPV16/18werestudiedusingimmunohistochemicalstaining.Allspecimensofthecondylomaacuminatumcasewerepositiveforexpressionofp53andp21^WAH1.Theexpressionofp53incondylomaacuminatuminfectedbyHPV16/18wassignificantlylowerthanthatinspecimensinfectedbyHPV6/ll.However,expressionofp21wAHbetweenthetwogroupswasnotsignificantlydifferent.Expressionofp53incondylomaacuminatumislikelyrelatedtoHPVgenotype,expressionofp21^WAH1wasnotrelatedtoHPVgenotype.

简介：寻常狼疮是皮肤结核中最常见的类型,约占所有皮肤结核患者的半数。本病易侵犯儿童和青少年,好发于面部,影响容貌。我们用异烟肼、乙胺丁醇和利福平联合治愈寻常狼疮2例,报告如下。

简介：色素失禁症又称色素失调症（incontinentiapigmenti，IP）、Bloch—Sulzberger综合征、Bloch—Sie—mens综合征[1]、真皮变性黑变病（melanosiscoriadegenerarativa），为伴发眼、骨骼系统和中枢神经系统发育缺陷的一种复合性遗传性综合征。现报道我中心确诊的2例婴儿色素失禁症病例。

简介：由于人体在昏睡、昏迷期局部长期压迫导致的皮肤软组织缺血性损伤并不常见,我科先后接诊2例该病患者,报告分析如下。

简介：Inordertoacknowledgethemulti-infectiondataofSTDpatientsandimprovetheprophylaxisandtherapyforSTDs，297patientsand30healthypeoplewereexaminedusingtheHSV-2plasmaantibody．TheresultsshowedthatallkindsofSTDpatientswereinfectedbyHSV-2indifierentratios．thehighestratiooccurringinsyphilispatients．

简介：Objective:TounderstandthechangeofTh1/Th2andTc1/Tc2balanceinpatientswithsecondaryearlysyphilis.Methods:PeripheralbloodThandTccellsubpopulationswereexaminedbythree-colorflowcytometryin30patientswithsecondaryearlysyphilisand20healthysubjects.Results:PercentagesofCD3^+,CD4^+TcellsandtheratioofCD4^+/CD8^+andNKcellsinsyphiliticpatientsweresignificantlylowerthaninthecontrolgroup(P<0.001),butnostatisticaldifferencewasfoundinpercentagesofCD8^+andCD19^+cellsbetweenthetwogroups(P>0.05).ThepercentageofTh1cellsandratiosofThl/Th2andTcl/Tc2insyphiliticpatientswereclearlydecreasedascomparedtothoseincontrois(P<0.001-0.01),whereaspercentagesofTc0andTc2cellsinsyphiliticswashigherthanthoseincontrols(P<0.001).Conclusion:Therecouldbeadisturbanceofcellularimmunityandtherelativelyintactfunctionofhumoralimmunityinsecondarysyphilis.ItispostulatedthatthepolarizationtowarddevelopmentofTh2andTc2cellsandtheirassociatedcytokinesmightbeinvolvedintheincompleteclearanceoftreponemesandthedevelopmentofchronicinfection.

简介：报告2例女性外阴血管角皮瘤,患者分别为58岁和69岁,临床表现均为大阴唇出现多个紫红色、蓝黑色类圆形丘疹。病理表现均为表皮角化过度,棘层不规则增生,真皮浅层可见扩张的血管腔,内充满红细胞。诊断：血管角皮瘤。

简介：Objective:Toconstructarecombinantplasmidcontainingtheoutermembraneprotein2(Omp2)geneofChlamydiatrachomatisandexpressOmp2inE.coli.Methods:Theomp2geneofC.trachomatisserovarDwasclonedintopQE30vectorfollowingPCRamplificationfromgenomicDNA.E.coliM15transformantswereinducedtoexpressthefusionproteinbyIPTGandtheproductwasidentifiedbySDS-PAGEandWesternblot.Results:ConfirmedbyenzymecleavageanalysisandDNAsequencing,acorrectrecombinantplasmidpQE30/omp2wasconstructed.Thefusionproteinfromthetransformantswasapproximately60kDainsizeinSDS-PAGEanalysis,whichcouldspeciallyreactwithanti-6×HismousemonoclonalIgGantibodies.Conclusion:WesuccessfullyexpressedOmp2inE.coilM15,providinganefficientandsimplesystemforassayingtheimmunologicalpropertiesofOmp2.

简介：Objective:Tostudythecellularimmunitystatusofpatientswithrecurrentgenitalherpes.Methods:Serumlevelsofinterlukin-2anditssolublereceptorandinterlukin-6weremeasuredbyELISAin34patientswithrecurrentgenitalherpes.Results:SerumlevelsofIL-2andIL-6weresignificantlydecreasedinpatientscomparedtohealthycontrols(P<0.01),andthelevelofsIL-2Rwassignificantlyincreasedinpatientswithrecurrentgenitalherpes(P<0.01).Therewerenosignificantdifferencesinallvariablesamongstpatientsregardingrelapsestageandremissionstage(P>0.05).Conclusion:Therewasacellularimmunedeficiencyinpatientswithrecurrentgenitalherpes.

简介：例1,男,37岁。面颈、双上肢多发暗红色丘疹伴瘙痒5个月。皮肤科情况:面部、颈部、双上肢泛发孤立红色丘疹,粟粒至黄豆大小,质韧。例2,女,53岁。臀部红色丘疹2年。皮肤科情况:面部、颈部、肘部、臀部皮肤可见散在红色、暗紫色丘疹、斑块,约豆粒至钱币大小,有浸润感,质韧。两例患者皮肤镜下均可见橘黄色无结构区、白色线条及白色瘢痕样色素减退、线状血管及线状分支状血管;皮损组织病理检查:真皮可见大量上皮样细胞组成的'裸结节',结节周围少量淋巴细胞浸润,散在的多核巨细胞。诊断:皮肤结节病。

简介：ObjectivesTocomparetheefficiencyoffourcommercialELISAkitsindetectingtype-specificHSV-2IgGantibodies.MaterialsandMethods:Atotalof125subjects,including105withgenitalulcers,and20controlswithoutanyhistoryofSTDswererecruitedfromtheSTDclinicfordetectionoftype-specificHSV-2IgGantibodywithdifferentkits.FourkindsofcommerciallyavailableELISAkits,includingQuidaHSV2IgGELISA(AifulangBiochemCo.Ltd.,Hangzhou),TORCH-HSV2IgG(JingmeiBiotechCo.Ltd.,Shanghai),Captia^TMHSV2IgG(Trinitybiotech,USA)andHerpeSelectT^TM2ELISAlgG(Focustechnologies,USA)wereusedforevaluation.WesternBlotassaywasperformedasagoldstandard.Results:ComparedtoWesternBlotresults,thesensitivityandspecificityofthekits(QuidaHSV2,TORCHHSV2,Captia^TMHSV2andHerpeSelect^TM2)were13.1%and98.4%,7.5%and100%,100%and11.1%,87.7%and96.7%,respectively.Thepositivepredictivevalue(PV)andnegativePVofthefourkitswere88.9%and54.3%,100%and55.5%,55.6%and100%,96.2%and89.2%,respectively.TheareasundertheROCcurveofthreekits(QuidaHSV2,Captia^TMHSV2andHerpeSelect^TM2)were0.885(0.822-0.948),0.825(0.747-0.902),0.974(0.950--0.998),respectivelyConclusion:TheperformanceofHerpeSelect^TM2isthebestamongthefourkits.Theresultsalsoindicatethatthecommerciallyavailablekitsfordetectionoftype-specificHSV2antibodyshouldbere-evaluatedintermsoftheirvalidity.

简介：Objective:Toinvestigatetherolesofinterleukin-2(IL-2)andinterleukin-10(IL-10)inpathogenesisofearlysyphilis.Methods:TheserumlevelsofIL-2andIL-10in48patientswithearlysyphilisweredetectedbyABC-ELISA.Results:(1)ThelevelofIL-2inthepatientswithearlysyphiliswassignificantlyhigherthanthatinhealthycontrols,whilethatofIL-10waslower(P<0.001andP<0.001).(2)ThelevelsofIL-2andIL-10werealmostidenticalinpatientswithprimaryandsecondarysyphilis(P>0.05),aswellasbetweendif-ferentRPRtiters(P>0.05).(3)Aftertherapy,thelevelofIL-2decreasedmarkedly(P<0.05),whilethatofIL-10increase(p>0.05).(4)AsignificantcorrelationwasfoundbetweentheserumlevelsofIL-2andIL-10(r=0.5385P<0.05).Conclusions:Th1up-regulationoccursinpatientswithearlysyphilis,andplaysanactiveroleinfightingagainstTPinfection.

简介：大疱性类天疱疮（bullouspemphigoid，BP）是一种好发于老年人的表皮下大疱病。作者近来诊治的2例肾移植术后并发大疱性类天疱疮，均为中青年患者，现报告如下：

简介：乳房外Paget病（EMPD）是一种罕见的，发生于乳房外的一种表皮内存在Paget细胞的湿疹样癌。可发生于外阴、阴囊、腹股沟、阴茎、肛周等大汗腺丰富的部位，常常与表皮内肿瘤和内脏肿瘤有关，由于表现多种多样易出现误诊。在病理上应与Bowen病，浅表型恶性黑色素瘤区别，发生于外阴的乳房外Paget病在临床上更易误诊为湿疹，脂溢性皮炎。本文介绍了2例被误诊的Paget病，同时复习了国内外有关本病的一些诊断和治疗方面的进展。

简介：水痘和带状疱疹分别是水痘-带状疱疹病毒（VZV）原发感染和再活化引起不同临床表现的2种疾病。水痘传染性强，而带状疱疹的传染性则尚未十分明确。本文报告一组带状疱疹患者家庭内2名密切接触者2周内相继患水痘的病例。

简介：报告2例结节型皮肤肥大细胞增生症患儿。2例患儿均为出生后不久发病,表现为躯干和四肢散发的结节性皮损,部分皮损表面可出现水疱。皮肤组织病理表现为真皮浅层或中层弥漫肥大细胞浸润,Giemsa染色可见肥大细胞内紫红色异染颗粒。结合病史、临床表现和组织病理学改变诊断为结节型皮肤肥大细胞增生症。