简介：Cerebralischemianotonlycausespathologicalchangesintheischemicareasbutalsoinducesaseriesofsecondarychangesinmoredistalbrainregions(suchasthecontralateralcerebralhemisphere).Theimpactofsupratentoriallesions,whicharethemostcommontypeoflesion,onthecontralateralcerebellumhasbeenstudiedinpatientsbypositronemissiontomography,singlephotonemissioncomputedtomography,magneticresonanceimaginganddiffusiontensorimaging.Inthepresentstudy,weinvestigatedmetabolitechangesinthecontralateralcerebralhemisphereaftersupratentorialunilateralischemiausingnuclearmagneticresonancespectroscopy-basedmetabonomics.Thepermanentmiddlecerebralarteryocclusionmodelofischemicstrokewasestablishedinrats.Ratswererandomlydividedintothemiddlecerebralarteryocclusion1-,3-,9-and24-hourgroupsandtheshamgroup.~1Hnuclearmagneticresonancespectroscopywasusedtodetectmetabolitesintheleftandrightcerebralhemispheres.Comparedwiththeshamgroup,theconcentrationsoflactate,alanine,γ-aminobutyricacid,cholineandglycineintheischemiccerebralhemispherewereincreasedintheacutestage,whiletheconcentrationsofN-acetylaspartate,creatinine,glutamateandaspartateweredecreased.Thisdemonstratesthatthereisanupregulationofanaerobicglycolysis(shownbytheincreaseinlactate),aperturbationofcholinemetabolism(suggestedbytheincreaseincholine),neuronalcelldamage(shownbythedecreaseinN-acetylaspartate)andneurotransmitterimbalance(evidencedbytheincreaseinγ-aminobutyricacidandglycineandbythedecreaseinglutamateandaspartate)intheacutestageofcerebralischemia.Inthecontralateralhemisphere,theconcentrationsoflactate,alanine,glycine,cholineandaspartatewereincreased,whiletheconcentrationsofγ-aminobutyricacid,glutamateandcreatinineweredecreased.Thissuggeststhatthereisadifferenceinthemetabolitechangesinducedbyischemicinjuryinthecontral

简介：Theangiotensin-convertingenzymegeneisacandidategeneofstroke.Thepresentstudyinvolved62healthyvolunteersand148patientswithmiddlecerebralarterystenosisasconfirmedbybraincolorultrasoundfromaHanpopulationinNorthChina,anddeterminedtheperipheralbloodangiotensin-convertingenzymegenotypeusingPCR-restrictionfragmentlengthpolymorphismanalysis.TheresultsshowedthatthefrequenciesoftheDDgenotypeandDallelewereincreasedinpatientswithmiddlecerebralarterystenosis,butthedifferencewasnotstatisticallysignificantcomparedwithhealthycontrols.ThefindingsofthisstudyontherelationshipbetweenstrokegenesandmiddlecerebralarterystenosisindicatenosignificantcorrelationbetweenthefrequenciesoftheDDgenotypeandDalleleofangiotensin-convertingenzymeandmiddlecerebralarterystenosisinthisHanpopulationfromNorthChina.Inthefuture,studieswillbecarriedouttoinvestigatecorrelationsbetweenmultiplestrokecandidategenesynergyandmiddlecerebralarterystenosistoprovideafoundationforthedevelopmentofgenetherapy.

简介：CurrentevidenceshowsthatapolipoproteinE(APOE),apolipoproteinCI(APOC1)andlowdensitylipoproteinreceptor-relatedprotein(LRP)variationsarerelatedtolate-onsetAlzheimer’sdisease.However,itremainsunclearifgeneticpolymorphismsinthesegenesareassociatedwithcognitivedeclineinlate-onsetAlzheimer’sdiseasepatients.Weperformeda30-monthlongitudinalcohortstudytoinvestigatetherelationshipbetweenAlzheimer’sdiseaseandAPOE,APOC1,andLRP.Inthisstudy,78ChineseHanpatientswithlate-onsetAlzheimer’sdiseasewererecruitedformGuangxiZhuangAutonomousRegioninChina.APOE,APOC1,andLRPgenotypingwasperformedusingpolymerasechainreaction-restrictionfragmentlengthpolymorphisms.TheMini-MentalStateExaminationandClinicalDementiaRatingScalewereusedtoassesspatients’cognitivefunction.Aftera30-monthfollow-upperiod,wefoundasignificantreductioninMini-MentalStateExaminationtotalscore,ahigherproportionofpatientsfulfillingcognitiveimpairmentprogressioncriteria,andahigherproportionofAPOC1H2carriersinAPOEε4carrierscomparedwithnon-carriers.Inaddition,theAPOEε4allelefrequencywassignificantlyhigherinthecognitiveimpairmentprogressiongroupcomparedwiththenon-cognitiveimpairmentprogressiongroup.Inconclusion,APOEε4playsanimportantroleinaugmentingcognitivedecline,andAPOC1H2mayactsynergisticallywithAPOEε4inincreasingtheriskofcognitivedeclineinChinesepatientswithlate-onsetAlzheimer’sdisease.

简介：Theselectionofelectro-acupunctureparametersremainspoorlyunifiedbetweenclinicalstudies.Thepresentstudyobservedtheeffectsofelectro-acupuncturingRenzhong(DU26)withdifferentstimulationparametersonmotorfunctionrecoveryfollowingmiddlearteryocclusioninjuryinrats.ResultsshowedanoptimalstimulationparameterforRenzhongelectro-acupuncturethatwaslowfrequencyandmildcurrent(2Hz,1mA)significantlyimprovedcorticalexcitabilityandconductivefunction,andpromotedrecoveryinaratmodelofmotorfunctioninmiddlearteryocclusion.Frequencyhadagreaterimpactthancurrentorinteraction,andplayedacriticalroleinelectro-acupuncturetherapy.