简介：Objective:ToinvestigatethemembranelocalizationfunctionoftheCX26proteinwhenits86thaminoacidisThr,SerorArg,anditsrelationstodeafness.Methods:CX26-GFPproteinwitheitherThr,SerorArgasthe86thaminoacidwasexpressedinmouseSGNcellsviatheGFPfusiontypelentivirusexpressionsystem.Themembranelocalizationofthefusionproteinwasobservedunderafluorescencemicroscope.Results:ThemutatedproteinofCX26T86Swaslocalizedtocellmembraneandformgapconjunctionstructures,showingnodifferencetothewildtypeCX26protein(withThrasthe86thaminoacid).However,thegapconjunctionstructuredisappearedwhenthemutationwasCX26T86A.Conclusion:TheseresultsindicatethattheCX26T86Rmutationmaybeacauseofhearingloss,butCX26T86Sasanon-pathogenicpolymorphismmutationdoesnotaffectfunctionsoftheCX26protein.Theresultsareinaccordancewiththeresultsofclinicalscreening.

简介：Durationisasalientfeatureofacousticsignalsincludingspeech.Durationtuningwasfirstreportedinfrogsandlaterinecholocatingbats.Morerecently,durationtuninghasbeenreportedinnon-echolocatingmammalsandappearstobeafundamentalencodingmechanismthroughouttheanimalkingdom.However,thedurationtuningreportedinthesenon-echolocatingmammalsappearstobemuchweakerthanthatinthepreviousstudiesonbats.Incontrasttothisfinding,ourrecentstudyreportedthatdurationtuningintheICinguineapigsappearedtobestrongwhenitwasmeasuredusinganappropriatetemporalwindow.Withsuchatemporalwindow,durationtuningwasfoundtobecompatiblewiththatofecho-locatingbats.Inthepresentreport,wefurtherdemonstratethatdurationtuningintheICofthisspeciesisestablishedbyinteractionbetweenexcitationandGABAergicinhibition.Inadditiontooverallincreaseinresponsiveness,applicationofbicuculline(BIC),aGABA-Areceptorantagonist,wasfoundtosignificantlyreduceoreliminatedurationselectivityin44outofthe67neuronsthatshowedcleardurationtuningfromasampleof340neurons.