简介:Soilmicrobialbiomassandcommunitystructuresarecommonlyusedasindicatorsforsoilqualityandfertility.Ainvestigationwasperformedtostudytheeffectsoflong-termnaturalrestoration,cropping,andbarefallowmanagementsonthesoilmicrobialbiomassandbacterialcommunitystructuresindepthsof0–10,20–30,and40–50cminablacksoil(Mollisol).Microbialbiomasswasestimatedfromchloroformfumigation-extraction,andbacterialcommunitystructuresweredeterminedbyanalysisof16SrDNAusingpolymerasechainreaction-denaturinggradientgelelectrophoresis(PCR-DGGE).Experimentalresultsshowedthatmicrobialbiomasssignificantlydeclinedwithsoildepthinthemanagementsofrestorationandcropping,butnotinthebarefallow.DGGEprofilesindicatedthatthebandnumberintop0–10cmsoilswaslessthanthatindepthof20–30or40–50cm.Thesesuggestedthatthemicrobialpopulationwashighbutthebacterialcommunitystructurewassimpleinthetopsoil.ClusterandprinciplecomponentanalysisbasedonDGGEbandingpatternsshowedthatthebacterialcommunitystructurewasa?ectedbysoildepthmoreprimarilythanbymanagements,andthesuccessionofbacterialcommunityasincreaseofsoildepthhasasimilartendencyinthethreemanagements.FourteenpredominatingDGGEbandswereexcisedandsequenced,inwhich6bandswereidentifiedasthetaxaofVerrucomicrobia,2bandsasActinobacteria,2bandsasα-Proteobacteria,andtheother4bandsasδ-Proteobacteria,Acidobacteria,Nitrospira,andunclassifiedbacteria.Inaddition,thesequencesof11DGGEbandswerecloselyrelatedtounculturedbacteria.Thus,thebacterialcommunitystructureinblacksoilwasstable,andthepredominatingbacterialgroupswereuncultured.
简介:在福建省东山县滨海沙地,对连栽条件下木麻黄林地根际和非根际土壤化学性质和酶活性进行了对比研究。结果表明:二代木麻黄林的根际和非根际土壤pH值均小于一代林,表明木麻黄连栽引起根际土壤酸化;根际土壤有机质、CEC值、水解性总酸度、全氮、水解氮、速效钾、交换性Mg^2+和ca^2+含量随着木麻黄连栽而降低,且心土层(20-40cm)差异更大,但土壤全磷、全钾变化不大;与一代林相比,二代木麻黄根际土壤磷酸酶和过氧化物酶活性减弱,多酚氧化酶活性增大,脲酶无明显变化。第二代木麻黄林地根际土壤养分的消耗、生物活性的降低和有毒物质的累积等,可能造成土壤肥力衰退,林木生长受阻。
简介:矿产资源开采造成了严重的重金属污染,为选择合理植被恢复模式以修复土壤重金属污染,以淄博市四宝山破坏山体不同植被恢复模式为对象,测定土壤有机质、全氮、碱解氮、有效磷、速效钾质量分数,pH值等主要化学性质,土壤多酚氧化酶、过氧化氢酶、过氧化物酶、脲酶活性和Cu、Zn、Cd等7种重金属质量分数,分析不同植被恢复模式对土壤重金属质量分数及土壤酶活性的影响。结果表明:1)实验区土壤中重金属Zn轻度污染,Cu和Cd重度污染,其他重金属均不构成污染;2)各人工植被恢复模式土壤重金属质量分数均低于灌草丛的,其中黑松林对土壤Cu、Cd的修复效果最好,侧柏林对土壤Zn的修复效果最好;3)土壤重金属全量在垂直分布上规律复杂,有效量均表现为下层高于上层,土壤有效Cu、有效Zn、有效Cd下层比上层依次高9.24%~18.94%、0.97%~20.09%和5.48%~35.51%;4)在影响土壤酶活性的各种因素中,破坏山体土壤重金属有效量与土壤化学性质的影响最为明显,处于同等重要的地位。本研究发现,Cu、Zn、Cd均对4种土壤酶活性表现出了抑制作用,建议用土壤多酚氧化酶、过氧化氢酶、脲酶活性作为破坏山体土壤重金属Cu污染的评价指标。