简介:为分离筛选能够降解TSNAs的微生物,探讨其降解特性,本研究采用替代底物平板稀释法分离和靶标底物点接法筛选相结合的分离筛选策略,筛选到能够利用NNK为唯一碳源和氮源而生长的细菌05-5402菌株,鉴定为短小芽孢杆菌(Bacilluspumilus)。白肋烟浸提液经05-5402菌株处理后,TSNAs含量降低了22.2%,其中NNK和NAT的含量分别降低了47.4%和55.7%。晾制的烟叶经05-5402菌株处理后,TSNAs降低17.3%,其中,NAB的含量降低了52.2%。发酵过程中的烟丝经05-5402菌株处理,TSNAs含量下降12.2%。05-5402菌株能实现烟草特有亚硝胺的高效定向降解,具有较大的应用潜力。
简介:Leafshapesarenotonlytheusefulindicatorsinplanttaxonomy,butalsotheimportantfactorsaffectingenergyandmaterialexchangeinleaves.Inthispaper,wecollectedandscannedtheleavesofNitrariatangutoruminDengkouofInnerMongoliaAutonomousRegion(themeanannualprecipitation145mm)andMinqinofGansuProvince(themeanannualprecipitation115mm)andN.sphaerocarpainDunhuang,andthenanalyzedleafshapeparameterswithImage-ProPlus6.0imageprocessingsoftwareandleafδ13CvaluesintheisotopelaboratoryoftheChineseAcademyofForestry.Theresultshowedthat:1)asleafareaincreasedwithincreasingwateravailabilitytheincreasesintheleaflengthandwidthwereasynchronously;2)withthesameleafwidth,the1eavesofN.tangutorumandN.sphaerocarpaweresignificantlylongerinhighwateravailableconditions;and3)althoughthereweresignificantlydifferencesinwateravailabilitybetweenDengkouandMinqin,aswellasbetweenthebottomandmiddleofthealluvialfanneartheEastLakeinDunhuang,theleafδ13CvaluesofN.tangutorumorN.sphaerocarpaweresimilarindifferentwaterconditions(P>0.05).Ourresultssuggestedthattheratioofleafperimetertoareawouldbeanimportantfactorwhichlinkedleafshapetoplantwaterphysiology.Duringgrowingprocedureofleafarea,leaflengthincreasewaspriortoitswidthtoalleviatethereductioninratioofperimetertoareaandmaintainwateruseefficiencyoftheplant.
简介:Aerobicstaticpilecomposting(mechanicalturningevery3days)ofpigmanurewaspreparedat8m3windrowheaps.Sawdustwasusedasthebulkingagenttoprovideadditionalcarbonandtoincreasetheporosityofthesubstrate.TwotreatmentsatinitialC/Nratiosof30and15,respectively,weredesignedinthestudy.Dissolvedorganiccarbon(DOC),solubleNH4+-N,C/Nratiosinsolidandaqueousphases,E4/E6ratios,andseedgerminationindex(GI)weredeterminedtoevaluatethematurityoftheco-composts.Seedgerminationindex,abiologicalparameter,wassuggestedasoneofthemostreliablematurityindicatorsfororganiccompost.TheresultsshowedthatthetreatmentattheinitialC/Nratioof30reachedmaturityafter49daysofcomposting;however,thetreatmentattheinitialC/Nratioof15shouldrequirecompostingtimeoflongerthan63daystoobtainmaturation.Chemicalmulti-indicatorevaluationwasnecessary,andtheGImeasurementwastherecommendedapproachformaturityevaluationinthestudy.
简介:维生素C,又称丙种维生素,VitaminumC,抗坏血酸(Ascorbicacid),简称维C或Vc。维生素C是一种酸性己糖衍生物。化学名称为L—3—氧带苏己糖醛酸内酯。分子式为C_6H_8O_6,分子量为176.1。1维生素C的性状外观为白色结晶粉末,有酸味,久置色渐变微黄色,易溶于水,水溶液呈酸性反应。稍溶于乙醇,微溶于甘油,不溶于乙醚和氯仿。熔点为190℃。抗坏血酸钠为白色或微黄色结晶粉末,略带酸味,只溶于水,基本上不溶于乙醇、乙醚等。在220℃时分解。维生素C是动物的重要营养成分之一,是维持机体正常代谢和生理机能不可缺少的物质。维生素C参与动物体内氧化还原过程,影响核酸的形成,又是某些酶和辅酶的组成部分。它是增进机体非特异性免疫反应的辅助药物。它具有多方面的作用与功能。
简介:GPS12C/XLC应用于森林调查是今后森林调查的发展方向,对今后森林资源一类,二类调查时广泛使用GPS12C/XLC提出建议。
简介:为了使基于人工模拟降雨装置的水土保持试验更可靠,采用人工降雨试验方法,通过研究QYJY-503C人工模拟降雨装置的降雨特性,探讨该装置在水土保持科学试验中的适用性。结果表明:1)雨滴达到终速时雨滴-色斑直径的关系为d=0.3839D0.709;2)QYJY-503C人工模拟降雨装置有效降雨均匀度超过80%;3)在试验范围内,雨滴直径随降雨强度增大而增大,雨滴中数直径与降雨强度呈d50=0.5595I0.2805的幂函数关系,相同降雨强度条件下的雨滴直径较天然雨滴偏小20%~70%;4)降雨动能与降雨强度呈E=0.0042I-0.021的线性关系,可通过控制降雨强度实现降雨动能与天然降雨的相似性;5)QYJY-503C人工模拟降雨装置性能稳定、可控性强,可适用于室内土壤侵蚀的研究。
简介:Thepromoterregionofadroughtandabscisicacid(ABA)induciblegene,osr40c1,wasisolatedfromasalt-tolerantindicaricevarietyPokkali,whichis670bpupstreamoftheputativetranslationstartcodon.Insilicopromoteranalysisofresultedsequenceshowedthatatleast15typesofputativemotifsweredistributedwithinthesequence,includingtwotypesofcommonpromoterelements,TATAandCAATboxes.Additionally,severalputativecis-acingregulatoryelementswhichmaybeinvolvedinregulationofosr40c1expressionunderdifferentconditionswerefoundinthe5′-upstreamregionofosr40c1.TheseareABA-responsiveelement,light-responsiveelements(ATCT-motif,BoxI,G-box,GT1-motif,Gap-boxandSp1),myeloblastosisoncogeneresponseelement(CCAAT-box),auxinresponsiveelement(TGA-element),gibberellin-responsiveelement(GARE-motif)andfungal-elicitorresponsiveelements(BoxEandBox-W1).Aputativeregulatoryelement,requiredforendosperm-specificpatternofgeneexpressiondesignatedasSkn-1motif,wasalsodetectedinthePokkaliosr40c1promoterregion.Inconclusion,thebioinformaticanalysisofosr40c1promoterregionisolatedfromindicaricevarietyPokkaliledtotheidentificationofseveralimportantstress-responsivecisactingregulatoryelements,andtherefore,theisolatedpromotersequencecouldbeemployedinricegenetictransformationtomediateexpressionofabioticstressinducedgenes.
简介:以便揭示在二CCDD染色体种,Oryzaalta和Oryzalatifolia之间的起源和进化关系,在situ杂交(鱼)的荧光被采用从O与C0t-1DNA分析二种的染色体。alta作为一根探针。Karyotype比较地也在O之间被分析。alta和O。latifolia基于他们杂交的类似的乐队模式发信号。在O之间有高相同和靠近的关系。alta和O。然而,在杂交之间的区别表明的latifolia也是清楚的。C0t-1DNA被证明是种类--并且染色体类型特定。C0t-1DNA鱼能是更有效的分析在不同种类之间的genomic关系,这被建议。根据在二allotetraploidy种之间的高度并且中等重复的DNA序列的比较分析,O。alta和O。latifolia,可能的起源和Oryza的allotetraploidy的进化机制被讨论。
简介:Thechloroformfumigation-incubationmethowasusedtomeasurthesoilmicrobialbiomassC(SMBC)andN(SMBN)in16loessialsoilssampledfromAnsai,YongshouandYanglinginShaanxiProvince.TheSMBContentsinthesoilsrangedfrom75.9to301.0μgCg^-1withanaverageof206.μgCg^-1,accountingfor1.36%-6.24%ofthetotalsoilorganicCwithanaverageof3.07%,andtheSMBNcontentsfrom0.51to68.40μgNg^-1withanaverageof29.4μgNg^-1,accountingfor0.20%-5.65%ofthetotalNinthesoilswithanaverageof3.36%.AcloserelationshipwasfoundbetweenSMBCandSMBN,andtheybothwerepositivelycorrelatedwithtotalorganicC,totalN,NaOHhydrolizableNandmineralizableN.Theseresultsconfirmedthasoilmicrobialbiomasshadacomparativeroleinnutrientcyclesofsoils.
简介:本研究以二倍体马铃薯C为母本,E为父本的杂交F1群体共90个株系,构建了一张包含12个连锁群和78个SSR标记,总长度1141.99cM,标记间平均距离14.64cM的遗传图谱。结合2015年和2016年鉴定的与块茎建成相关性状表型数据,采用QTLIciMappingV4.1软件的完备区间作图法(ICIM)进行QTL定位和效应估计。共检测14个与株高、根长、匍匐茎长、匍匐茎数、微型薯数相关QTL。表型变异解释率为3.05%~19.19%。有5个QTL在2个环境中被重复检测到,其中在5号染色体上有2个(qTN5-1,qTN5-2)与微型薯相关的QTL,遗传贡献率为9.64%和9.67%。在9号染色上有3个(qSL9-1,qSL9-2,qSL9-3)与匍匐茎长相关的QTL,遗传贡献率为3.05%~14.17%。