简介：Recentadvancesinstemcelltechnologieshaveopenednewavenuesforthetreatmentofanumberofdiseasesstilllackingeffectivetherapeuticoptions.Celltransplantationhasemergedasamongthemostpromisingclinicalinterventionfordisorderssuchasinjuries,diabetes,liver

简介：Traumaticinjuriestospinalcordelicitdiversesignalingpathwaysleadingtounselectiveandcomplexpathologicaloutcomes:deathofmultipleclassesofneuralcells,formationofcysticcavitiesandglialscars,disruptionofaxonalconnections,anddemyelinationofsparedaxons,allofwhichcancontributemoreorlesstodebilitatingfunctionalimpairmentsfoundinpatientswithspinal

简介：Redbloodcells(RBC)’flickeringpresentthedynamicpropertiesofthecytomembrane.Itscomplexitycouldbeusedforaginganalysisortheevaluationforthestoragequality.Theflickeringactivityisakindofreversibleperpendicularmotionofthespecifiedpixel.Therefore,thecomplexityanalysisdependsonthereliabledetectionoftemporalvariationforthegray-scalevaluesfromeachpixelofthecells.Inthispaper,weimprovedourpreviousworkonthescreeningofthehorizontaldriftedcellswithasurfacebasedoncellregistrationmethodandtheeffectofGSMexposuretothedynamicpropertiesoftheRBCsintermsofmulti-scalesampleentropywaspresentedinthepaper.

简介：Cancercelldormancyisthemaincauseofcancerrecurrenceandfailureoftherapyasdormantcellsevadenotonlytheanticancerdrugsbutalsothehostimmunesystem.Thesedormantcellsveilthemselvesfromdetectionbyimagingand/orusingbiomarkers,whichimposesanadditionalproblemintargetingsuchcells.Asimilarformofhibernationprocessknownasencystationisstudiedindetailforpathogenicunicellulareukaryoticmicroorganisms.Byexaminationusingmicroarraygeneexpressionprofiles,immunocytochemistrytools,andsiRNAsduringtheprocessofencystation,understandingthecovertfeaturesofcancercelldormancyasproposedcouldbepossible.Thisknowledgecanbeextendedtodormantcancercellstouncoverthemechanismsthatunderliethisghost,yetdangerousstateofhumancancers.Weproposeastrategytoinducedormancyandexitthisstatebyapplicationofknowledgegainedfromtheencystationinductionandretrievalprocessesinpathogeniceukaryoticmicroorganisms.Giventhatearlydetectionandcharacterizationofdormantmalignanttumorcellsisimportantasageneralstrategytomonitorandpreventthedevelopmentofovertmetastaticdisease,thishomologymayenablethedesignoftherapiesthatcouldeitherawakethedormantcellfromdormancytomakeitavailablefortherapiesorprolongsuchaphasetomakecancerappearasachronicdisease

简介：作为一名专职从事STEM教育项目／课程和本土实践研究的科研人员和一线课堂观察员，我从2012年投身STEM教育研宄至今。最初在西度搜索中输入关键词“STEM教育”，只能搜到“STEM干细胞”相关信息；而到2015年5月，再次在百度搜索中输入关键词“STEM教育”，可以获得9，510，000个相关结果，STEM教育这个源于美国国家教育战略的“舶来品”在我国也越来越受到广泛的关注。

简介：Receptor-ligandinteractionsinbloodflowarecrucialtoinitiatesuchbiologicalprocessesasinflammatorycascade,plateletthrombosis,aswellastumormetastasis.Tomediatecelladhesion,theinteractingreceptorsandligandsmustbeanchoredontotwoapposingsurfacesoftwocellsoracellandasubstratum,i.e.,two-dimensional(2D)binding,whichisdifferentfromthebindingofasolubleligandinfluidphasetoareceptor,i.e.,three-dimensional(3D)binding.Whilenumerousworkshavebeenfocusedon3Dkineticsofreceptor-ligandinteractionsintheimmunesystem,2Dkineticsanditsregulationshavebeenlessunderstood,sincenotheoreticalframeworkorexperimentalassayswereestablisheduntil1993.Notonlydoesthemolecularstructuredominate2Dbindingkinetics,buttheshearforceinbloodflowalsoregulatescelladhesionmediatedbyinteractingreceptorsandligands.Here,weprovideanoverviewofcurrentprogressin2Dbindingandregulations,mainlyfromourgroup.Relevantissuesoftheoreticalframeworks,experimentalmeasurements,kineticratesandbindingaffinities,andforceregulationsarediscussed.

简介：某些疾病被知道引起变化在物理并且房间的biomechanical性质。这些在其它之中包括癌症，疟疾，和镰刀房间贫血症。典型地，如此的物理性质变化能导致房间僵硬的几褶层增加或减少，它是重要的并且能导致严重病理和最终的灾难的故障身体功能。当有时开发了生物化学、生物的试金检测疾病的发作或存在，总是有需要开发更快速、精确、敏感的方法检测并且诊断疾病。Biomechanical性质变化能起一个重要作用在这方面。因此，对疾病biomechanics的研究不能仅仅给我们位于疾病前进下面的机制的深入的知识，但是能也为察觉和诊断用作一个强大的工具。这篇文章提供一些卓见进机会因为在疾病的发作或前进期间在细胞的机械性质换掉怎么重要，能为察觉和诊断作为有用工具被利用。我们将也展示已经被开发了执行如此的察觉和诊断的几种技术。

简介：许多癌症都依赖于EGFR（表皮生长因子受体）来维持旺盛生长。一些被期望通过关闭这一细胞信号接收位点来起作用的药物,却在大多数患者中没有能够阻止住癌症。在正常细胞中,EGFR发挥功能可使细胞受益。然而在大量的癌症中--从较常见的卵巢癌和皮肤癌到较少见的侵袭性癌症如大脑胶质母细胞瘤--控制EGFR的基因往往被重编程,并生成了太多的EGFR,由此导致了失控性的生长以及癌细胞扩散。

简介：Circulatingtumorcells(CTCs)areapopulationoftumorcellsmediatingmetastasis,whichresultsinmostofthecancerrelateddeaths.ThenumberofCTCsintheperipheralbloodofpatientsisrare,andmanyplatformshavebeenlaunchedfordetectionandenrichmentofCTCs.EnumerationofCTCshasalreadybeenusedasaprognosismarkerpredictingthesurvivalrateofcancerpatients.YetCTCsshouldbemorepotential.StudiesonCTCsatsinglecelllevelmayhelprevealingtheunderlyingmechanismoftumorigenesisandmetastasis.Thoughfarfromdeveloped,thisareaofstudyholdsmuchpromiseinprovidingnewclinicalapplicationanddeepunderstandingtowardsmetastasisandcancerdevelopment.

简介：Objective:Thisstudyaimstoexploretheclinicopathologicfeaturesof112patientswithmantlecelllymphoma(MCL).Methods:Datafrom112MCLcaseswerecollected,andimmunohistochemicalassaywasconducted.Fluorescenceinsituhybridization(FISH)detectedabreakintheCCND1gene.Thet-testwasusedinthestatisticalanalysis.Results:Alltumorcellsinthe112casesexpressedBcell-relatedantigen,including1blastoidsubtypeand1polymorphicsubtype.Amongallcases,106expressedCD5and104expressedcyclinD1.AbreakintheCCND1genewasnotfoundin3caseswithCD5-MCL.IgH/CCND1polyploidwasobservedin2classiccases.Conclusion:MCLisatypeofspecialimmunophenotypicB-celllymphoma.Theprognosesofblastoidandpolymorphicsubtypesarepoor.Specialsubtypesshouldbeclassifiedduringdiagnosis.

简介：Cell-celljunctionsinthecochleaarehighlycomplexandwellorganized.Theroleofthesejunctionsistomaintainstructuralandfunctionalintegrityofthecochlea.Inthisreview,wedescribeclassificationofcelljunction-associatedproteinsidentifiedwithinthecochleaandprovideabriefoverviewofthefunctionoftheseproteinsinadherentjunctions,gapjunctionsandtightjunctions.

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简介：AIMTo评估β的可靠性；在试验性的绿内障model.METHODSGlaucoma老鼠的一个网膜的中心房间(RGC)标记当模特儿的-III-Tubulin蛋白质被把聚苯乙烯microbeads注入C57BL/6J老鼠的前面的房间建立，在intraocular压力(IOP)被提高以后，当时，他们的视网膜是获得的14d和28d。网膜的扁平的山和节是由fluorogold(FG)和β的双labeled；-III-Tubulin抗体或由β标记单人赛；-III-Tubulin抗体，然后，RGC被认为并且比较注射眼睛的respectively.RESULTSIOP显著地被提高并且在22.8±到达了山峰；0.7公里Hg在白天14在注射以后，然后落下到11.3±；0.7公里Hg在白天28。RGC数字由标记的FG和β数了；-III-Tubulin抗体标记是64807±；4930和64614±；5054分别地在控制组织，没有重要差别。在白天14，RGC在里面试验性的组与控制组相比显著地减少了，但是标记数的FG和β之间没有重要差别；在试验性的组或在控制组把数的任何一个标记的-III-Tubulin抗体。结果在白天是类似的28，与进一步的RGCloss.CONCLUSIONOur，结果建议β；-III-Tubulin蛋白质没被IOP举起影响并且能在绿内障的试验性的模型为RGC被用作一个可靠标记。

简介：在这份报纸，我们使用任意的Riemann解答者，它不能满足Maire的要求，到Maire发展在的基于节点的Lagrangian计划[P.H。Maire等，暹罗J。Sci。Comput，29(2007)，1781-1824]。特别地，我们使用所谓的多液体隧道onAveraged体积(MFCAV)Riemann解答者和适应地把MFCAV解答者与另外的更消散的Riemann解答者相结合到Maire的计划的一个Riemann解答者。任何一个二个解答者都不满足Maire的要求，这被注意。数字实验被介绍证明二个Riemann解答者的应用程序是成功的。[从作者抽象]

简介：Objective:Patientswithheadandneckcanceroftensufferfrommalnutrition.Thisstudyaimstoinvestigatetheinfluenceofbodymassindex(BMI)ontheprognosisoflaryngealsquamouscellcarcinoma(LSCC).Methods:Atotalof473patientswithLSCCinitiallytreatedatSunYat-senUniversityCancerCenterbetweenJanuary2005andJuly2009wereretrospectivelyreviewed.SurvivalanalysiswasperformedbytheKaplan-MeiermethodandCoxregressionmodel.Results:LowBMIbeforetreatmentwassignificantlyassociatedwithpooroverallsurvivalinpatientswithLSCC(P<0.001).BMIwasanindependentprognosticfactorforpatientswithLSCC.Conclusion:LeannessbeforetreatmentwasassociatedwithpoorprognosisinpatientswithLSCC.GoodnutritionalstatusisfavorabletoimprovesurvivalinpatientswithLSCC.

简介：Peripheralnerveinjuries(PNI)areamajorclinicalproblem.Ingeneral,PNIresultsfrommotorvehicleaccidents,lacerationswithsharpobjects,penetratingtrauma(gunshotwounds)andstretchingorcrushingtraumaandfractures.ItisestimatedthatPNIoccurin2.8%oftraumapatientsandthisnumberreaches5%ifplexusandrootlesionsarein-

简介：Nafion-stabilizedPtnanoparticlecolloidalsolutionissynthesizedthroughethyleneglycolreduction.Pt/Nafionaddedwithcarbonblackaselectricconductionmaterial(labeledPt/Nafion-XC72)showsexcellentelectrochemicalpropertycomparedwithPt/C.Aftera300-cycledischargingdurabilitytest,thecellperformanceofmembraneelectrodeassembly(MEA)withthePt/Nafion-XC72andPt/Ccatalystsindicatesa29.9%and92.2%decrease,respectively.ThechargetransferresistancesofPt/Nafion-XC72andPt/Cincreaseby27.2%and101.9%,respectively.TheremainingelectrochemicallyactivesurfaceareaofPtisabout61.7%inPt/Nafion-XC72andabout38.1%inPt/Cafterthedurabilitytest.TheparticlesizeofPt/Cincreasesfromabout5.1nmtoabout10.8nmbutonlyfrom3.6nmto5.8nminthecaseofPt/Nafion-XC72.ThesedatasuggestthatPt/Nafion-XC72asacatalystcanenhancethedurabilityofPEMFCscomparedwithPt/C.

简介：Renalcellcarcinoma(RCC)accountsforapproximately3%ofallcancercases.RCCsusuallymetastasizetothelungs,bones,liver,orbrain.Only<1%ofpatientswithbonemetastasesmanifestedclavicularRCCmetastases.Thus,clavicularmetastasisastheinitialpresentationofRCCisextremelyrare.WereportapatientwithRCCmetastasistotheleftclavicle,whichwasfirstpresentedwithpaincausedbyapathologicalfracture.Magneticresonanceimagerevealedarenaltumor,andtechnetium-99m–methylenediphosphonatebonescintigraphyshowedmultipleosseousmetastases.Thepatienteventuallyunderwentsurgerytoremovethelateralendoftheleftclavicleandrightkidney.Histopathologyrevealedrenaltumorandclearcellcarcinomaintheclavicle.Finally,wereview17casesofclavicularmetastasesoriginatingfromdifferentmalignancies.

简介：Recently,RNAprocessinghasemergedasanovelpathwaythatmaycontributetothemaintenanceofgenomestability[1].Alternativesplicingisakeymolecularmechanismforincreasingthefunctionaldiversityoftheeukaryoticproteomes,butitalsooftenalteredincancer.Mountingevidenceindicatesthatalternativesplicing,theprocessthatallowsproductionofmultiplemRNAvariantsfromeachgene,contributestotheheterogeneityofthedisease[2].Althoughthemechanismofalternativesplicingvariantincancerisunclear,thecancer-specificalternativesplicingvariantshavebeenobservedinavarietyofhumancancersandcancercelllinesandhavebeenconnectedtotumorgenesis.

简介：自发的术语劳动在包括cytokine生产和白血球渗入的myometrium与放大煽动性的事件被联系；然而，调整如此的事件的潜在的机制充分没被理解。我们由成长胎儿假设了子宫的墙的那机械段由子宫的myocytes通过各种各样的cytokines的版本便于外部白血球溢出进术语myometrium。人的myometrial房间(hTERT-HM)受到静态的机械段；调节段的媒介被收集并且分析了使用48-plexLuminex试金和ELISA。人的子宫的microvascularendothelial房间(UtMVEC-Myo)的房间粘附分子表示上的调节段的媒介的效果被量的聚合酶链反应(qPCR)和流动cytometry检测；测试leukocyte-endothelial相互作用的功能的试金：到endothelial房间和象THP-1monocytic房间的移植一样的标记calcein的主要人的neutrophils的transendothelial移植的白血球的粘附被萤光计估计。在vitro学习的水流证明机械段(i)直接由hTERT-HM细胞导致多重cytokines和chemokines的分泌物(IL-6，CXCL8，CXCL1，移植禁止的因素(MIF)，VEGF，G-CSF，IL-12p70，bFGF和导出血小板的生长因素子单元B(PDGF-bb)，P<；0.05)；导致段的cytokines(ii)提高白血球粘附到包围的内皮细胞层子宫的微脉管系统由(iii)导致endothelial房间粘附分子的表示并且(iv)指导外部白血球的transendothelial迁居。(vi)抵销Chemokine抗体和用途广泛的chemokine禁止者堵住白血球移植。我们的数据从子宫的血容器为白血球招募提供机械规定的一个证明给myometrium，为白血球建议通常认为的机制在劳动和产后的复杂物期间渗透到子宫。